Abstract
Background:
The plant under investigation (Tetracera sarmentosa) is a dicotyledonous flowering plant and belongs to the family Dilleniaceae. The goal of our investigation was to determine whether the leaf extracts of this plant held any significant medicinal properties.
Methods:
Leaves of T. sarmentosa were extracted with pure ethanol (EETS) and methanol (METS), and then methanol extract fractioned with n-hexane (NHFMETS) and chloroform (CHFMETS). The extracts and fractions were tested for antioxidant activity, which was measured by using qualitative and quantitative procedures. Thrombolytic activity was evaluated by the clot lysis test. Analgesic activity was evaluated employing the acidic acid-induced writhing test, the formalin-induced paw licking test and tail immersion on Swiss albino mice. The anti-inflammatory activity test was studied using the paw edema test. Forced swimming, tail suspension, elevated plus maze and hole board model tests were used to evaluate neuropharmacological activity.
Results:
All the extracts and fractions possessed antioxidant effects. All the extracts, fractions and streptokinase exhibited significant (p<0.0001) clot lysis. The extracts and fractions produced significant analgesic effects as evaluated by the acetic acid writhing test, the formalin-induced paw licking test and the tail immersion method. Similarly, carrageenan-induced inflammation was significantly antagonized by the treatments. The extracts and fractions also significantly showed neuropharmacological (antidepressant and anxiolytic) effects.
Conclusions:
The overall results suggested that this plant deserves further investigation to isolate the active compounds which are responsible for these activities and to establish the mechanism of action.
Acknowledgments
The authors are greatly thankful to the managing committee of the Department of Pharmacy, University of Chittagong, Bangladesh, for providing all the laboratory amenities and for the support through a research grant to accomplish the study (grant no. 180-Pharm-P&D-7-32/36). The authors are also thankful to all the members of GUSTO (a research group) for their help in the experiment.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. This work was carried out in collaboration between all the authors. M.T.H. and M.S.A. collected the plant leaves and prepared the extracts and fractions. M.M.N.U., M.S.H.K., M.H. and M.R.H. carried out the study design and performed the experiments, the data collection, the data interpretation, the manuscript preparation and the statistical analysis. Z.A.M. designed and planned the studies and supervised the experiments. N.M.M.A.B. and F.A. participated in the experiments, the data collection and the literature search. Z.A.M. supervised the whole study, the design and the data interpretation. Z.A.M. acted as the corresponding author. All the authors read and approved the final version of the manuscript.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; the collection, analysis and interpretation of the data; the writing of the report or the decision to submit the report for publication.
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