Abstract
Background:Murraya koenigii (Rutaceae) (curry patta: Hindi) of the family Rutaceae is used in the traditional Indian system of medicine for its immunomodulatory properties. The essential oil of the leaves of M. koenigii possesses antimicrobial, antifungal, and pesticidal activities and is used for the treatment of amebiasis, diabetes, and hepatitis. The present study was performed to evaluate the effect of M. koenigii on humoral and cell-mediated immune responses in rats.
Methods: Aqueous extract of M. koenigii leaves was administered orally in a dose of 350 mg/kg. Cell-mediated immunity was assessed by measuring foot pad thickness following sensitization by injection of keyhole limpet hemocyanin and subsequent challenge by the same. Humoral immunity was assessed by measurement of hemagglutination titer to sheep red blood cells (SRBCs).
Results: In the humoral immune response, the administration of M. koenigii [350 mg/kg per os (p.o.)] from day 1 to day 7 after sensitization with SRBC on day 0 caused a significant increase in the primary anti-SRBC titer. However, the secondary immune response was decreased significantly (p<0.05) as shown by a decrease in secondary anti-SRBC titer measured on day 11 following a booster dose of antigen on day 8. In the delayed-type hypersensitivity test, M. koenigii (350 mg/kg, p.o.), when administered for 14 days, produced a significant (p<0.05) decrease in foot pad thickness when compared with the control group.
Conclusions: Thus, these results suggest that oral administration of M. koenigii augments primary humoral immune response and decreases cell-mediated immunity.
References
1. Gupta S, George M, Singhal M, Sharma GN, Garg V. Leaves extract of Murraya koenigii Linn. for anti-inflammatory and analgesic activity in animal models. J Adv Pharm Technol Res 2010;1:68–77.Search in Google Scholar
2. Tembhurne SV, Sakarkar DM. Effects of Murraya koenigii leaf extract on impaired gastrointestinal motility in streptozotocin-induced diabetic rats. Zhong Xi Yi Jie He Xue Bao 2011;9:913–9.10.3736/jcim20110814Search in Google Scholar
3. Tembhurne SV, Sakarkar DM. Influence of Murraya koenigii on experimental model of diabetes and progression of neuropathic pain. Res Pharm Sci 2010;5:41–7.Search in Google Scholar
4. Arulselvan P, Senthilkumar GP, Sathish Kumar D, Subramanian S. Antidiabetic effect of Murraya koenigii leaves on streptozotocin-induced diabetic rats. Pharmazie 2006;61:874–7.Search in Google Scholar
5. Arulselvan P, Subramanian SP. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultrastructural changes of pancreatic β-cells in experimental diabetes in rats. Chem Biol Interact 2007;165:155–64.10.1016/j.cbi.2006.10.014Search in Google Scholar
6. Math MV, Balasubramaniam P. The hypoglycaemic effect of curry leaves (Murraya koenigii spreng). Indian J Physiol Pharmacol 2005;49:241–2.Search in Google Scholar
7. Sathaye S, Bagul Y, Gupta S, Kaur H, Redkar R. Hepatoprotective effects of aqueous leaf extract and crude isolates of Murraya koenigii against in vitro ethanol-induced hepatotoxicity model. Exp Toxic Pathol 2011;63:587–91.10.1016/j.etp.2010.04.012Search in Google Scholar
8. Mitra E, Ghosh AK, Ghosh D, Mukherjee D, Chattopadhyay A, Dutta S, et al. Protective effect of aqueous Curry leaf (Murraya koenigii) extract against cadmium-induced oxidative stress in rat heart. Food Chem Toxicol 2012;50:1340–53.10.1016/j.fct.2012.01.048Search in Google Scholar
9. Desai SN, Patel DK, Devkar RV, Patel PV, Ramachandran AV. Hepatoprotective potential of polyphenol rich extract of Murraya koenigii L.: an in vivo study. Food Chem Toxicol 2012;50:310–4.10.1016/j.fct.2011.10.063Search in Google Scholar
10. Trease GE, Evans WC. Pharmacognosy, 14th ed. London: Harcourt Brace and Company, 1996:293.Search in Google Scholar
11. Halder S, Mehta AK, Mediratta PK. Augmented humoral immune response and decreased cell-mediated immunity by Aloe vera in rats. Inflammopharmacology 2012;20:343–6.10.1007/s10787-012-0134-8Search in Google Scholar
12. Halder S, Mehta AK, Mediratta PK, Sharma KK. Essential oil of Clove (Eugenia caryophyllata) augments the humoral immune response but decreases cell mediated immunity. Phytother Res 2011;25:1254–6.10.1002/ptr.3412Search in Google Scholar
13. Mediratta PK, Sharma KK, Singh S. Evaluation of immunomodulatory potential of Ocimum sanctum seed oil and its possible mechanism of action. J Ethnopharmacol 2002;80:15–20.10.1016/S0378-8741(01)00373-7Search in Google Scholar
14. Insistoris L, Siroki O, Underger U, Basaran N, Desi I. Immunotoxicological investigations on rats treated subacutely with dimethoate. As3+ and Mg2+ in combination. Hum Exp Toxicol 2001;20:329–36.Search in Google Scholar
15. Wagner H, Proksch A. Immunostimulatory drugs of fungi and higher plants. In: Wagner H, Hikino H, Farnsworth NR, editors. Economic and medicinal plant research. London: Academic Press, Vol. I, 1985:113–53.Search in Google Scholar
16. Miller LE. Introduction to immunology. In: Ludke HR, Peacock JE, Tomar RH, editors. Manual of laboratory immunology. London: Lea and Febiger, 1991:1–18.Search in Google Scholar
17. Nagappan T, Ramasamy P, Wahid ME, Segaran TC, Vairappan CS. Biological activity of carbazole alkaloids and essential oil of Murraya koenigii against antibiotic resistant microbes and cancer cell lines. Molecules 2011;16:9651–64.10.3390/molecules16119651Search in Google Scholar PubMed PubMed Central
18. Tachibana Y, Kikuzaki H, Lajis NH, Nakatani N. Antioxidative activity of carbazoles from Murraya koenigii leaves. J Agric Food Chem 2001;49:5589–94.10.1021/jf010621rSearch in Google Scholar PubMed
19. Kumar NS, Mukherjee PK, Bhadra S, Saha BP, Pal BC. Acetylcholinesterase inhibitory potential of a carbazole alkaloid, mahanimbine, from Murraya koenigii. Phytother Res 2010;24:629–31.10.1002/ptr.3023Search in Google Scholar PubMed
20. Singh AP, Wilson T, Luthria D, Freeman MR, Scott RM, Bilenker D, et al. LC-MS-MS characterisation of curry leaf flavonols and antioxidant activity. Food Chem 2011;127:80–5.10.1016/j.foodchem.2010.12.091Search in Google Scholar
21. Zaveri M, Gohil P, Jain S. Immunostimulant activity of n-butanol fraction of root bark of Oroxylum indicum, vent. J Immunotoxicol 2006;3:83–99.10.1080/15476910600725942Search in Google Scholar PubMed
22. Agrawal PK, Reynolds DL. Reynolds evaluation of the cell mediated immune response of chickens vaccinated with Newcastle disease virus as determined by the under agarose leukocyte-migration-inhibition technique. Avian Dis 1991;35:360–4.10.2307/1591190Search in Google Scholar
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