Abstract
A synergetic method integrating both pulsed electric field (PEF) and ozone treatment was developed as a novel approach to investigate the degradation of high molecular weight chitosan (Mw=4.5×105 Da). A device integrating both components was designed and assembled for the experiments. Results showed that the highest degradation percentage of chitosan was achieved with PEF/ozone co-treatment generated at experimental conditions of 1.2 L/min of ozone flow rate, 100 mL/min of 0.6% (w/v) chitosan solution flow rate, and 26.7 kV/cm of PEF intensity. The degradation percentage after 60 min PEF treatment was 24.89%, whereas it was improved to 94.89% by ozone treatment for 60 min. Combining the two treatments resulted in enhanced degradation percentage of 99.56%, with low molecular weights sample (Mw<2,500 Da) obtained. FTIR analysis demonstrated that the amide structure of the degradation products was minimally affected by the co-treatment. XRD pattern indicated that the crystallinity of the degradation products decreased. In addition, it could complete dissolve in water after 60 min PEF/ozone co-treatment. These results demonstrated the synergetic PEF/ozone co-treatment as an effective method for degradation of high molecular weight chitosan.
Acknowledgments
This research was supported by the Chinese National Science Fund (61001057, 21376094, and 31301559), the Chinese National “863” project (2011AA100801), the Science and Technology Program of Guangdong, China (2008A024200002 and 2009B090200031), and the Fundamental Research Funds for the Central Universities, China (2013ZM0025).
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