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Licensed Unlicensed Requires Authentication Published by De Gruyter April 5, 2019

Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin

  • Amr Abouelezz , David Micinski , Aino Lipponen and Pirta Hotulainen EMAIL logo
From the journal Biological Chemistry

Abstract

The axon initial segment (AIS) comprises a sub-membranous lattice containing periodic actin rings. The overall AIS structure is insensitive to actin-disrupting drugs, but the effects of actin-disrupting drugs on actin rings lack consensus. We examined the effect of latrunculin A and B on the actin cytoskeleton of neurons in culture and actin rings in the AIS. Both latrunculin A and B markedly reduced the overall amount of F-actin in treated neurons in a dose-dependent manner, but the periodicity of actin rings remained unaffected. The insensitivity of AIS actin rings to latrunculin suggests they are relatively stable.

Acknowledgments

We thank Rimante Minkeviciene and Iryna Hlushchenko for their help with neuronal culture preparation. All imaging was performed using microscopes in the Biomedicum Imaging Unit. This work was supported by the Academy of Finland (Funder Id: http://dx.doi.org/10.13039/501100005876, P.H., SA 266351), Doctoral Programme Brain and Mind (A.A.), and Minerva Foundation (Funder Id: http://dx.doi.org/10.13039/501100005876, Grant Number: 307735, P.H., D.M.).

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2019-0111).


Received: 2019-01-15
Accepted: 2019-03-06
Published Online: 2019-04-05
Published in Print: 2019-08-27

©2019 Walter de Gruyter GmbH, Berlin/Boston

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