Abstract
Circular RNA (circRNA) play a crucial role in many biological processes and have been proved as potential biomarkers and therapeutic targets in many diseases. Manipulation of their expression is a critical task. In this study, we developed a new strategy for circRNA suppression with DNAzyme. Data showed single-digestion DNAzymes cleaved circRNA efficiently in vitro but not in cell culture. However, tandem DNAzymes for double digestion showed higher cleavage efficacy both in vitro and in cell culture. Functional study demonstrated that double-digestion DNAzymes suppressed the miRNA sponge function of circRNA and changed the proliferation and migration rates of HCC cells.
Acknowledgments
We thank Xiangya Experiment Center (Changsha, China) for providing the cell lines. We also thank Geneseed Biotech Co., Ltd. (Guangzhou, China) for constructing the circRNA OE plasmid. This research is supported by National Natural Science Foundation of China for ‘Functional studies of miR-183/-96/-182 cluster in breast cancer diagnosis and treatment’ (Grant No. C0709-31201056), Hunan Provincial Natural Science Foundation of China (Grant No. 2018JJ2493) and the Innovation Driven Project of Central South University (Grant No. 20170030010004).
Conflict of interest statement:
The authors declare no conflict of interest.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2018-0232).
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