Abstract
Bleached birch kraft pulp (BKP, Södra Cell AB, Sweden) and unmodified bacterial cellulose (BC) pellicles, biosynthesized by the bacterium Komagataeibacter rhaeticus, were converted to cellulose nanofibers via ammonium persulfate (APS) oxidation. Fiber dimensions were investigated in an atomic force microscope, and the crystallite size was calculated by Rietveld analysis. Saos-2 osteosarcoma cell line served to assess the in vitro cytocompatibility of the biocomposite films. Results showed that individual cellulose nanofibers with an average width of 80±15 nm and a length between 600 and 1200 nm are formed by APS oxidation. The obtained BC nanofibers can be promising constituents in nanocellulose films and in chitosan-matrix films with improved physical-mechanical and biological properties. Good cellular biocompatibility was found for chitosan/oxidized cellulose films; the viability of Saos-2 osteosarcoma cells was higher on chitosan/oxidized BC films compared to chitosan/oxidized BKP films.
Acknowledgments
Financial support for this research from 6.VPP “ResProd” project Nr.3. “Biomaterials and products from forest resources with versatile applicability” is greatly appreciated. The authors are grateful to Madara Saka, Institute of Microbiology and Biotechnology, University of Latvia, for production of bacterial cellulose, and Laura Vikele, State Education Development Agency, Latvia, for unselfish voluntary assistance in this study. Linda Vecbiskena would like to express her appreciation to COST Action FP1205 Grant Holder for financial support to realize a Short-Term Scientific Mission at the Department of Chemistry, Materials and Chemical Engineering “G.Natta”, Milan, Italy (COST-STSM-FP1205-26877). Linda Vecbiskena is thankful to Lina Altomare, Luigi de Nardo and Gabriele Candiani, Department of Chemistry, Materials and Chemical Engineering “G.Natta”, Milan, Italy, for the cooperation to perform the cell culture work.
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