Skip to main content
Log in

Far infrared imaging, an effective way to screen maize seedling mutants for drought stress response

  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Water shortage is a global challenge and affects crop growth and development seriously. Stoma is the main channel of plant water transpiration. Water transpiration through stomata is an endothermic process and affects temperature of plants, especially leaf surface. In this study, we established a method for screening maize seedling mutant with abnormal leaf temperature by far infrared imaging. We found that seedling mutants with abnormal leaf temperature manifested different drought tolerance. Mutants with lower leaf temperature demonstrated faster moisture loss rate, poorer drought tolerance, higher osmotic potential, lower leaf relative water content, more accumulation of hydrogen peroxide, more serious cell membrane damage and more robust root systems and biomass under drought treatment, which are opposite in maize seedling mutants with higher leaf surface temperature compared with the corresponding control. Taken together, the method we established is an effective way to screen maize mutants with abnormal drought response.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Belin C., de Franco P.O., Bourbousse C., Chaignepain S., Schmitter J.M., Vavasseur A., Giraudat J., Barbier-Brygoo H. & Thomine S. 2006. Identification of features regulating OST1 kinase activity and OST1 function in guard cells. PlantPhysiol. 141: 1316–1327.

    CAS  Google Scholar 

  • Brennan T. & Frenkel C. 1977. Involvement of hydrogen peroxide in the regulation of senescence in pear. PlantPhysiol. 59: 411–416.

    CAS  Google Scholar 

  • Chen Z.Z., Zhang H.R., Jablonowski D., Zhou X.F., Ren X.Z., Hong X., Schaffrath R., Zhu J.K. & Gong Z. 2006. Mutations in ABO1/ELO2, a subunit of holo-elongator, increase abscisic acid sensitivity and drought tolerance in Arabidopsis thaliana. Mol. CellBiol. 26: 6902–6912.

    CAS  Google Scholar 

  • Chugh V., Kaur N. & Gupta A.K. 2011. Evaluation of oxidative stress tolerance in maize (Zea mays L.) seedlings in response to drought. Ind. J. Biochem. Biophys. 48: 47–53.

    CAS  Google Scholar 

  • Cominelli E., Conti L., Tonelli C. & Galbiati M. 2013. Challenges and perspectives to improve crop drought and salinity tolerance. N. Biotechnol. 30(4): 355–361.

    Article  Google Scholar 

  • Gaxiola R.A., Li J., Undurraga S., Dang L.M., Allen G.J., Alper S.L. & Fink G.R. 2001. Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Proc. Natl. Acad. Sci. USA 98: 11444–11449.

    Article  CAS  Google Scholar 

  • Gogorcena Y., Iturbe-Ormaetxe I., Escuredo P.R. & Becana M. 1995. Antioxidant defenses against activated oxygen in pea nodules subjected to water stress. PlantPhysiol. 108: 753–759.

    CAS  Google Scholar 

  • Janiak M. Kissel E., Van Dijck P. & Carpentier S. 2015. The unknown shadows of trehalase. Comm. Agric. Appl. Biol. Sci. 80: 71–76.

    CAS  Google Scholar 

  • Liu Y.K., Liu Y.B., Zhang M.Y. & Li DQ. 2010. Stomatal development and movement: the roles of MAPK signaling. PlantSignal Behav. 5: 1176–1180.

    Article  CAS  Google Scholar 

  • Merlot S., Mustilli A.C., Genty B., North H., Lefebvre V., Sotta B., Vavasseur A. & Giraudat J. 2002. Use of infrared thermal imaging to isolate Arabidopsis mutants defective in stomatal regulation. Plant J. 30: 601–609.

    Article  CAS  Google Scholar 

  • Miao Y., Lv D., Wang P., Wang X.C., Chen J., Miao C. & Song C.P. 2006. An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses. PlantCell 18: 2749–2766.

    CAS  Google Scholar 

  • Mustilli A.C., Merlot S., Vavasseur A., Fenzi F. & Giraudat J. 2002. Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. PlantCell 14: 3089–3099.

    CAS  Google Scholar 

  • Pallas J.E., Michel B.E. & Harris D.G. 1967. Photosynthesis, transpiration, leaf temperature, and stomatal activity of cotton plants under varying water potentials. PlantPhysiol. 42: 76–88.

    CAS  Google Scholar 

  • Price A.H., Cairns J.E., Horton P., Jones H.G. & Griffiths H. 2002. Linking drought-resistance mechanisms to drought avoidance in upland rice using a QTL approach: progress and new opportunities to integrate stomatal and mesophyll responses. J. Exp. Bot. 53: 989–1004.

    Article  CAS  Google Scholar 

  • Quan R.D., Shang M., Zhang H., Zhao Y.X. & Zhang J.R. 2004. Improved chilling tolerance by transformation with betA gene for the enhancement of glycinebetaine synthesis in maize. PlantSci. 166: 141–149.

    CAS  Google Scholar 

  • Rai G.K., Rai N.P., Rathaur S., Kumar S. & Singh M. 2013. Expression of rd29A::AtDREB1A/CBF3 in tomato alleviates drought-induced oxidative stress by regulating keyenzymatic and non-enzymatic antioxidants. PlantPhysiol. Biochem. 69: 90–100.

    CAS  Google Scholar 

  • Shou H., Bordallo P., Fan J.B., Yeakley J.M., Bibikova M., Sheen J. & Wang K. 2004. Expression of an active tobacco mitogen- activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize. Proc. Natl. Acad. Sci. USA 101: 3298–3303.

    Article  CAS  Google Scholar 

  • Sirichandra C., Davanture M., Turk B.E., Zivy M., Valot B., Leung J. & Merlot S. 2010. The Arabidopsis ABA-activated kinase OST1 phosphorylates the bZIP transcription factor ABF3 and creates a 14-3-3 binding site involved in its turnover. PLoSOne 5(11): e13935.

    Article  Google Scholar 

  • Song Y.W., Kang Y.L., Liu H., Zhao X.L., Wang P.T., An G.Y., Zhou Y., Miao C. & Song C.P. 2006. Identification and primary genetic analysis of Arabidopsis stomatal mutants in response to multiple stresses. Chin. Sci. Bull. 51: 2586–2594.

    Article  CAS  Google Scholar 

  • Spollen W.G., Lenoble M.E., Samuels T.D., Bemstein N. & Sharp R.E. 2000. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. PlantPhysiol. 122: 967–976.

    CAS  Google Scholar 

  • Vilela B., Moreno-Cortés A., Rabissi A., Leung J., Pagčs M. & Lumbreras V. 2013. The maize OST1 kinase homolog phos-phorylates and regulates the maize SNAC1-type transcription factor. PLoSOne 8(2): e58105.

    Article  Google Scholar 

  • Walton D.C., Harrison M.A. & Cote P. 1976. The effects of water stress on abscisic acid levels and metabolism in roots of Phaseolus vulgaris L. andother paints. Planta 131: 141–144.

    CAS  Google Scholar 

  • Wang W., Vinocur B., Shoseyov O. & Altman A. 2004. Role of plant heat shock proteins and molecular chaperones in the abiotic stress response. TrendsPlant Sci. 9: 244–252.

    CAS  Google Scholar 

  • Wang Y., Holroyd G., Hetherington A.M. & Ng C.K. 2004. Seeing “cool” and “hot”-infrared thermography as a tool for non- invasive, high-throughput screening of Arabidopsis guard cell signalling mutants. J. Exp. Bot. 55: 1187–1193.

    Article  CAS  Google Scholar 

  • Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Taka-hashi F. & Shinozaki K. 2006. The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis. J. Biol. Chem. 281: 5310–5318.

    Article  CAS  Google Scholar 

  • Zhang J. 1994. Accumulation of ABA in maize roots in response to root severing. NewPhytol. 127: 309–314.

    CAS  Google Scholar 

  • Zhang T., Chen S. & Harmon A.C. 2014. Protein phosphorylation in stomatal movement. PlantSignal. Behav. 9: e972845.

    Article  Google Scholar 

  • Zhu J.K. 2002. Salt and drought stress signal transduction in plants. Annu. Rev. Plant. Biol. 53: 247–273.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Baozhu Li or Chunpeng Song.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, B., Fan, R., Huang, S. et al. Far infrared imaging, an effective way to screen maize seedling mutants for drought stress response. Biologia 72, 1010–1016 (2017). https://doi.org/10.1515/biolog-2017-0111

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1515/biolog-2017-0111

Key words

Navigation