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Genomic and outer membrane protein diversity fingerprints of siderophore producing fluorescent Pseudomonas spp. using RAPD, Rep-PCR and SDS-PAGE profiling

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Abstract

Fluorescent Pseudomonas (FP) is an intrinsic group of plant growth promoting rhizobacteria (PGPR) which also has the potential to control phytopathogens. In the present study sixteen FP isolates were subjected to siderophore production and siderotyping. Four DNA finger printing analysis markers (RAPD, ERIC, BOX A1R, REP and GTG5) were used to assess their genetic diversity through DNA finger printing analysis. Outer membrane protein (OMP) homology of FP was carried out by SDS-PAGE analysis. Chemical characterization showed that the siderophores produced were hydroxamate in nature. Production of siderophores ranged from 21.73 ± 2.18 to 182.03 ± 1.66 μg mL−1 after 72 hr incubation. Maximum production was shown by the isolate M139. Dendrogram obtained from the combined binary data of 10 RAPD primers classified FPs into two distinct clusters. Except for M140, all the isolates from maize rhizosphere were grouped into the same cluster. Among three Rep-PCR analyses, REP primer showed maximum diversity in banding pattern. Combined binary data of three Rep primers also classified FP into two clusters as in RAPD and protein electrophenogram of the OMP. To the best of our knowledge this is the first report on genomic and OMP disparity among hydroxamate producing FPs.

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Acknowledgements

The authors are thankful to Jain University for the financial and infrastructure support. We are grateful to Dr. Vani R, Assistant Professor, Centre for Post Graduate Studies, Jain University Bangalore for her technical guidance.

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Authors and Affiliations

  1. Department of Biotechnology, Center for Post Graduate Studies, Jain University, Bangalore, India

    Nellickal Subramanian Jayamohan & Belur Satyan Kumudini

  2. Biocon Research Limited, Bangalore, India

    Shiragambi Hanamanthagouda Manohar

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  1. Nellickal Subramanian Jayamohan
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  2. Shiragambi Hanamanthagouda Manohar
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  3. Belur Satyan Kumudini
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Correspondence to Belur Satyan Kumudini.

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Jayamohan, N.S., Manohar, S.H. & Kumudini, B.S. Genomic and outer membrane protein diversity fingerprints of siderophore producing fluorescent Pseudomonas spp. using RAPD, Rep-PCR and SDS-PAGE profiling. Biologia 70, 1150–1158 (2015). https://doi.org/10.1515/biolog-2015-0133

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