Abstract
Background: Hei Yi (which means black worship and black dressing) Zhuang is a specific subgroup of the Zhuang nationality in China. Little is known about the relationship between genetic factors and lipid profiles in this population. Therefore, the present study was undertaken to compare the effects of lipoprotein lipase gene polymorphism at the PvuII locus on lipid levels in the Guangxi Hei Yi Zhuang and Han populations.
Methods: A total of 325 Hei Yi Zhuang subjects aged from 20 to 80years were surveyed using stratified randomized cluster sampling. Serum levels of lipids and apolipoproteins were measured. Gene polymorphism was determined using polymerase chain reaction and restriction fragment length polymorphism. The results were compared with those for 331 matched Han subjects living in the same district.
Results: Serum levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol and apolipoprotein B were significantly lower in Hei Yi Zhuang than in Han subjects (p<0.05–0.01), whereas the levels of high-density lipoprotein cholesterol and the ratio of apolipoprotein A1 to apolipoprotein B were significantly higher in Hei Yi Zhuang than in Han subjects (both p<0.01). The allelic frequencies for P+ and P– were 52.92% and 47.08% in Hei Yi Zhuang, and 58.46% and 41.54% in Han subjects (p<0.05), respectively. The frequencies of P+P+, P+P– and P–P– genotypes were 23.08%, 59.69% and 17.23% in Hei Yi Zhuang, and 29.31%, 58.31% and 12.38% in Han subjects (p>0.05), respectively. There were no significant differences or no significant correlation between serum lipid parameters and genotypes in Hei Yi Zhuang or Han subjects, or for the combined population of Hei Yi Zhuang and Han (all p>0.05).
Conclusions: The allelic frequencies of the lipoprotein lipase gene at the PvuII locus in Hei Yi Zhuang were different from those in Han subjects, but the genotypic frequencies in Hei Yi Zhuang subjects were not different from those in Han subjects. There was no significant correlation between polymorphism of the lipoprotein lipase gene at the PvuII site and serum lipid levels in the two ethnic groups.
Clin Chem Lab Med 2006;44:1416–21.
References
1. Kim HK, Chang SA, Choi EK, Kim YJ, Kim HS, Sohn DW, et al. Association between plasma lipids, and apolipoproteins and coronary artery disease: a cross-sectional study in a low-risk Korean population. Int J Cardiol 2005; 101:435–40.10.1016/j.ijcard.2004.03.057Search in Google Scholar
2. Hokanson JE, Austin MA. Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of population-based prospective studies. J Cardiovasc Risk 1996; 3:213–9.Search in Google Scholar
3. Marz W, Scharnagl H, Winkler K, Tiran A, Nauck M, Boehm BO, et al. Low-density lipoprotein triglycerides associated with low-grade systemic inflammation, adhesion molecules, and angiographic coronary artery disease: the Ludwigshafen Risk and Cardiovascular Health study. Circulation 2004; 110:3068–74.10.1161/01.CIR.0000146898.06923.80Search in Google Scholar
4. Kwiterovich PO Jr, Coresh J, Smith HH, Bachorik PS, Derby CA, Pearson TA. Comparison of the plasma levels of apolipoproteins B and A-1, and other risk factors in men and women with premature coronary artery disease. Am J Cardiol 1992; 69:1015–21.10.1016/0002-9149(92)90856-TSearch in Google Scholar
5. Assmann G, Schulte H. Relation of high-density lipoprotein cholesterol and triglycerides to incidence of atherosclerotic coronary artery disease (the PROCAM experience). Am J Cardiol 1992; 70:733–7.10.1016/0002-9149(92)90550-ISearch in Google Scholar
6. Bermudez OI, Velez-Carrasco W, Schaefer EJ, Tucker KL. Dietary and plasma lipid, lipoprotein, and apolipoprotein profiles among elderly Hispanics and non-Hispanics and their association with diabetes. Am J Clin Nutr 2002; 76:1214–21.10.1093/ajcn/76.6.1214Search in Google Scholar PubMed
7. Wu DM, Pai L, Sun PK, Hsu LL, Sun CA. Joint effects of alcohol consumption and cigarette smoking on atherogenic lipid and lipoprotein profiles: results from a study of Chinese male population in Taiwan. Eur J Epidemiol 2001; 17:629–35.10.1023/A:1015599129080Search in Google Scholar
8. Mohan V, Gokulakrishnan K, Deepa R, Shanthirani CS, Datta M. Association of physical inactivity with components of metabolic syndrome and coronary artery disease – the Chennai Urban Population Study (CUPS no. 15). Diabet Med 2005; 22:1206–11.10.1111/j.1464-5491.2005.01616.xSearch in Google Scholar PubMed
9. Austin MA, King MC, Bawol RD, Hulley SB, Friedman GD. Risk factors for coronary heart disease in adultfemale twins. Genetic heritability and shared environmental influences. Am J Epidemiol 1987; 125:308–18.10.1093/oxfordjournals.aje.a114531Search in Google Scholar PubMed
10. Srinivasan SR, Freedman DS, Webber LS, Berenson GS. Black-white differences in cholesterol levels of serum high-density lipoprotein subclasses among children: the Bogalusa Heart Study. Circulation 1987; 76:272–9.10.1161/01.CIR.76.2.272Search in Google Scholar
11. Auwerx J, Leroy P, Schoonjans K. Lipoprotein lipase: recent contributions from molecular biology. Crit Rev Clin Lab Sci 1992; 29:243–68.10.3109/10408369209114602Search in Google Scholar PubMed
12. Chamberlain JC, Thorn JA, Oka K, Galton DJ, Stocks J. DNA polymorphisms at the lipoprotein lipase gene: associations in normal and hypertriglyceridaemic subjects. Atherosclerosis 1989; 79:85–91.10.1016/0021-9150(89)90037-3Search in Google Scholar
13. Wang XL, McCredie RM, Wilcken DE. Common DNA polymorphisms at the lipoprotein lipase gene. Association with severity of coronary artery disease and diabetes. Circulation 1996; 93:1339–45.10.1161/01.CIR.93.7.1339Search in Google Scholar
14. Ye P, Pei L, Wang S. Polymorphisms of the human lipoprotein lipase gene: possible association with lipid levels in patients with coronary heart disease in Beijing area. Chin Med Sci J 1996; 11:157–61.Search in Google Scholar
15. Pasalic D, Sertic J, Kunovic B, Milicevic Z, Pasic A, Zrinski-Topic R, et al. Lipoprotein lipase gene polymorphism and lipid profile in patients with hypertriglyceridemia. Croat Med J 2001; 42:517–22.Search in Google Scholar
16. Peacock RE, Hamsten A, Nilsson-Ehle P, Humphries SE. Associations between lipoprotein lipase gene polymorphisms and plasma correlations of lipids, lipoproteins and lipase activities in young myocardial infarction survivors and age-matched healthy individuals from Sweden. Atherosclerosis 1992; 97:171–85.10.1016/0021-9150(92)90130-9Search in Google Scholar
17. Mitchell RJ, Earl L, Bray P, Fripp YJ, Williams J. DNA polymorphisms at the lipoprotein lipase gene and their association with quantitative variation in plasma high-density lipoproteins and triacylglycerides. Hum Biol 1994; 66:383–97.Search in Google Scholar
18. Thorn JA, Chamberlain JC, Alcolado JC, Oka K, Chan L, Stocks J, et al. Lipoprotein and hepatic lipase gene variants in coronary atherosclerosis. Atherosclerosis 1990; 85:55–60.10.1016/0021-9150(90)90182-ISearch in Google Scholar
19. Shimo-Nakanishi Y, Urabe T, Hattori N, Watanabe Y, Nagao T, Yokochi M, et al. Polymorphism of the lipoprotein lipase gene and risk of atherothrombotic cerebral infarction in the Japanese. Stroke 2001; 32:1481–6.10.1161/01.STR.32.7.1481Search in Google Scholar
20. Heinzmann C, Kirchgessner T, Kwiterovich PO, Ladias JA, Derby C, Antonarakis SE, et al. DNA polymorphism haplotypes of the human lipoprotein lipase gene: possible associations with high-density lipoprotein levels. Hum Genet 1991; 86:578–84.Search in Google Scholar
21. Yin R, Chen Y, Pan S, He F, Liu T, Yang D, et al. Comparison of lipid levels, hyperlipidemia prevalence and its risk factors between Guangxi Hei Yi Zhuang and Han populations. Arch Med Res 2006; 37:787–93.10.1016/j.arcmed.2005.12.005Search in Google Scholar
22. Yin R, Li R, Lin W, Yang D, Pan S. Effect of the MTP –493 G/T polymorphism on the lipid profiles of the Guangxi Hei Yi Zhuang and Han populations. Eur J Lipid Sci Technol 2006; 108:561–8.10.1002/ejlt.200500281Search in Google Scholar
23. Gotoda T, Yamada N, Murase T, Shimano H, Shimada M, Harada K, et al. Detection of three separate DNA polymorphisms in the human lipoprotein lipase gene by gene amplification and restriction endonuclease digestion. J Lipid Res 1992; 33:1067–72.10.1016/S0022-2275(20)41422-1Search in Google Scholar
24. Gotoda T, Senda M, Murase T, Yamada N, Takaku F, Furuichi Y. Gene polymorphism identified by Pvu II in familial lipoprotein lipase deficiency. Biochem Biophys Res Commun 1989; 164:1391–6.10.1016/0006-291X(89)91824-XSearch in Google Scholar
25. Ahn YI, Kamboh MI, Hamman RF, Cole SA, Ferrell RE. Two DNA polymorphisms in the lipoprotein lipase gene and their associations with factors related to cardiovascular disease. J Lipid Res 1993; 34:421–8.10.1016/S0022-2275(20)40733-3Search in Google Scholar
26. Dong W, Ma X, Zhang D, Yu S. Effect of maize embryo on delaying aging. Food Sci 2002; 23:95–7.Search in Google Scholar
27. Lairon D. Dietary fibres: effects on lipid metabolism and mechanisms of action. Eur J Clin Nutr 1996; 50:125–33.Search in Google Scholar
28. Liu Y, Zhang L, Wu Y. The dietary therapy for hyperlipidemia complicated with NIDDM. Chin J Clin Nutr 1995; 3:174–6.Search in Google Scholar
29. Zhang Y, Zhou Y, Wu F, Zhang M. The comparative effects of maize oil and lard on blood lipids serum glucose and brain lipofuscin in rats. Acta Nutrimenta Sinica 1996; 18:274–9.Search in Google Scholar
30. Grundy SM, Denke MA. Dietary influences on serum lipids and lipoproteins. J Lipid Res 1990; 31:1149–72.10.1016/S0022-2275(20)42625-2Search in Google Scholar
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