Clerodendrum viscosum is a traditionally used medicinal plant. The present study aimed to analyze a detailed cytotoxic effect of the non-polar petroleum ether fraction (AQPEF) of leaf aqueous extract of C. viscosum Vent. (LAECV) in Allium cepa root tip cells. The LAECV was fractionated with petroleum ether and tested for A. cepa toxicity. Micronuclei, polyploidy, and mitotic abnormalities were analyzed after AQPEF treatment. The AQPEF induced concentration-dependent increased mitotic abnormalities, micronuclei, and polyploid cell frequency in A. cepa root tip cells and that were comparable with the colchicine effects. Thus, the present study explores that petroleum ether is a suitable solvent for extraction of the active phytochemicals of LAECV; AQPEF having colchicine like micronuclei, polyploidy and mitotic abnormality inducing potentials in A. cepa root tip cells, indicating its perspective use in karyotyping, plant breeding programs, and cytotoxicity studies.