Chronic hyperadiponectinemia induced by transgenic overexpression increases plasma exosomes without significantly improving glucose and lipid metabolism

Keitaro Kawada; Shunbun Kita; Shiro Fukuda; Keita Fukuoka; Tomonori Okita; Emi Kawada-Horitani; Masahito Iioka; Taka-aki Sakaue; Yusuke Kawachi; Kohei Fujii; Yu Kimura; Shuichi Otabe; Yuya Fujishima; Hitoshi Nishizawa; Norikazu Maeda; Iichiro Shimomura

doi:10.1507/endocrj.EJ22-0665

ORIGINAL

Chronic hyperadiponectinemia induced by transgenic overexpression increases plasma exosomes without significantly improving glucose and lipid metabolism

Keitaro Kawada, Shunbun Kita , Shiro Fukuda , Keita Fukuoka, Tomonori Okita, Emi Kawada-Horitani, Masahito Iioka, Taka-aki Sakaue, Yusuke Kawachi, Kohei Fujii, Yu Kimura, Shuichi Otabe, Yuya Fujishima, Hitoshi Nishizawa, Norikazu Maeda, Iichiro Shimomura

Author information

Keitaro Kawada
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Shunbun Kita Corresponding author
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Department of Adipose Management, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Shiro Fukuda Corresponding author
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Keita Fukuoka
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Tomonori Okita
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Emi Kawada-Horitani
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Masahito Iioka
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Taka-aki Sakaue
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Yusuke Kawachi
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Kohei Fujii
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Yu Kimura
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Shuichi Otabe
Otabe Clinic, Fukuoka 811-1346, Japan
Yuya Fujishima
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Hitoshi Nishizawa
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Norikazu Maeda
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Department of Metabolism and Atherosclerosis, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
Iichiro Shimomura
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan

Keywords: Extracellular vesicles, Exosomes, Adiponectin, Glucose metabolism, Lipid metabolism

JOURNAL FREE ACCESS FULL-TEXT HTML

2023 Volume 70 Issue 6 Pages 635-645

DOI https://doi.org/10.1507/endocrj.EJ22-0665

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Abstract

The fat-derived factor, adiponectin, is considered a salutary circulating factor. We recently demonstrated that native adiponectin binds T-cadherin and promotes intracellular biogenesis and secretion of the exosome. Exosomes play important roles in various aspects of homeostasis, including glucose and energy metabolism. However, it remains unclear whether and how the promotion of exosome production by adiponectin in vivo is beneficial for glucose and lipid metabolism. In the present study, overexpression of human adiponectin in mice resulted in an increased number of circulating exosomes, but it did not significantly improve glucose metabolism, change body weights, or change triglyceride clearance under a high-fat diet. Multiple small doses of streptozotocin increased blood glucose and decreased triglyceride clearance similarly in both wild-type and transgenic mice. Thus, these results indicated that human adiponectin overexpression in mice increases plasma exosomes but does not significantly influence glucose and lipid metabolism.

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