Araştırma Makalesi
BibTex RIS Kaynak Göster

Türk SpliNPV-B İzolatından elde edilen lef-8 Geninin diğer Bakulovirüslerle Kısmi Sekans Karşılaştırması

Yıl 2005, Cilt: 11 Sayı: 04, 384 - 391, 01.11.2005

Umut Toprak İ. Alper Susurluk M. Oktay Gürkan

https://doi.org/10.1501/Tarimbil_0000000553

Öz

Spodoptera littoralis Boisd. Lepidoptera:Noctuidae nukleopolihedrovirus SpliNPV Baculoviridae’nin bir üyesi olup grup II NPV’leri arasında yer almaktadır. Lef-8 iyi korunmuş bir gen olup pek çok lepidopter NPV’sinde tanımlanmıştır. Kodlanan proteinlerin nükleotid ve aminoasit dizinleri ve gen içerikleri filogenetik olarak aydınlatıcı olabilmektedir. Bu çalışmada, Türk SpliNPV-B SpliNPV-TR1 genomundaki lef-8 geni PCR’la kısmi olarak çoğaltılmış ve aminoasit dizinlerine göre bazı bakulovirüslerle analiz edilmiştir. SpliNPV-TR1 kısmi lef-8 geninin aminoasit dizinleri SpltNPV ile % 84 benzelik gösterirken AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV ve BufuNPV ile sırasıyla sadece % 57, % 55 , % 51, % 50, % 50, % 50 ve % 49 aminoasit benzerliği göstermiştir. SpliNPV-TR1 ve granulovirüsler arasındaki sekanslar da düşük dizin benzerlikleri üretmiştir en fazla % 46 . Grup II NPV’leri esas olarak noctuid konukçulardan izole edilse de, bu noctuid NPV’leri arasında saptanan düşük dereceli benzerlikler SpliNPV-TR1 ve SpltNPV arasındaki yüksek benzerlik oranı hariç , bu izolatların monofiletik bir orijine sahip olamayacağını göstermektedir

Anahtar Kelimeler

Bakulovirüs Spodoptera littoralis Noctuidae SpliNPV-TR1 lef-8 sekans karşılaştırma

Kaynakça

  • Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI- BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25: 3389-3402.
  • Bulach D.M., C.A. Kumar, A. Zaia, B. Liang and D.E. Tribe. 1999. Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J. Invertebr. Pathol. 73: 59-73.
  • Chen X., W.F.J. IJkel, R. Tarchini, X. Sun, H. Sandbrink, H. Wang and S. Peters. 2001. The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J. Gen. Virol. 82: 241-257.
  • Cherry, C.L. and M.D. Summers. 1985. Genotypic variation among wild isolates of two nuclear polyhedrosis viruses from Spodoptera littoralis. J. Invertebr. Pathol. 46: 289-295.
  • Christen A.A. and B. Montalbano. 1990. Estimation of quantity of polymerase chain reaction products by ethidium bromide agarose plate assay. Biotechniques 9(3): 310.
  • Croizier G., K. Boukhoudmi-Amiri and L. Croizier. 1989. A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome. Arch. Virol. 104: 145-151.
  • El-Salamouny S., M. Lange, M. Jutzi, J. Huber and J.A. Jehle. 2003. Comparative study on the susceptibility of cutworms (Lepidoptera:Noctuidae) to Agrotis segetum nucleopolyhedrovirus and A. ipsilon nucleopolyhedrovirus. J. Invertebr. Pathol. 84: 75-82.
  • Faktor O. and B. Kamensky. 1997. Genomic localization and nucleotide sequence of a lef-8 gene of the Spodoptera littoralis nucleopolyhedrovirus. Virus genes 15 (1): 9-15.
  • Faktor O., M. Toister-Achituv and O. Nahum. 1997. Enhancer element, repetitive sequences and gene organization in an 8-kbp region containing the polyhedrin gene of the Spodoptera littoralis nucleopolyhedrovirus. Arch. Virol. 142: 1-15.
  • Friesen, P.D. and L.K. Miller. 1986. The regulation of baculovirus gene expression. Eds: pp. 31-50, W. Doerfler. and P. Boehm (eds). The molecular biology of baculoviruses. Springer-Verlag, Berlin,.
  • Guarino, L., J. Jin and W. Dong. 1998. Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase. J. Virol. 72: 10003-10010.
  • Herniou E.A., T. Luque, X. Chen, J.M. Vlak, D. Winstanley, J.S. Cory and D. O’Reilly. 2001. Use of whole genome sequence data to infer Baculovirus pylogeni. J. Virol. 17: 8117-8126.
  • Herniou E.A., J.A. Olezewski, J.S. Cory and D.R. O’Reilly. 2003. The genome sequence and evolution of baculoviruses. Annu. Rev. Entomol. 48: 211-234.
  • Herniou, E.A., J.A. Olezewski, D.R. O’Reilly and J.S. Cory. 2004. Ancient coevolution of baculoviruses and their insect hosts. J. Virol. 78: 3244-3251.
  • Hu Z., R. Broer, J. Westerlaken, J.W.M. Martens, F. Jin, L.M. Wang and J.M. Vlak. 1997. Identification and nucleotide sequence of the ecdysteroid UDP-glucosyltransferase gene of the Buzura suppressaria nucleopolyhedrovirus. Virus Res. 47: 91-97.
  • Huang J. and Levin D.B. 2001. Identification, transcription and sequence analysis of the Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) DNA polymerase gene Arch. Virol. 146: 303-326.
  • Huang, J., X. Liu and Levin D.B. 1999. Characterization of Spodoptera littoralis type B nucleopolyhedrovirus infection in selected insect cell lines. Arch. Virol. 144: 935-955.
  • Ilkel, W. F. J., E. A. Van Strien, J. G. M. Heldens, R. Broer, D. Zuidema, R. W. Goldbach and J. M. Vlak. 1999. Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J. Gen. Virol., 80: 3289-3304.
  • Jehle, J.A. 2004. The mosaic structure of the polyhedrin gene of the Autographa californica nucleopolyhedrovirus (AcMNPV). Virus Genes 29: 5-8.
  • Kislev, N. and M. Edelman. 1982. DNA restriction-pattern differences from geographic isolates of Spodoptera littoralis nuclear polyhedrosis virus. Virology 119: 219-222.
  • Lange, M. and J.A. Jehle. 2003. The genome of the Crptophlebia leucotreta granulovirus. Virology, 317: 220-236.
  • Lange, M., H. Wang, H. Zhihong and J.A. Jehle. 2004. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology 325: 36-47.
  • Levin D.B., M.A. Laitinen, C. Clarke, C.J. Lucarotti, B. Morin and I.S. Otvos. 1997. Characterization of nuclear polyhedrosis viruses from the three subspecies of Lambdina fiscellaria. J. Invertebr. Pathol. 69: 125-134.
  • Levin D.B. and B. Whittome. 2000. Codon usage in nucleopolyviruses. J. Gen. Virol. 81: 2313-2325.
  • Maeda, S., Y. Mukohara, and A. Kondo. 1990. Characteristically distinct isolates of the nuclear polyhedrosis virus from Spodoptera litura. J. Gen. Virol. 71: 2631-2639.
  • Martigoni, M.E. and P.J. Iwai. 1981. A catalogue of viral diseases of insects, mites, and ticks. Eds: pp. 897-911. H. D. Burges, Microbial control of pests and plant diseases 1970-1980. Academic Pres, London
  • Murphy, F.A., C.M. Fauquet, D.H.L. Biship, S.A. Ghabrial, A.W. Jarvis, G.P. Martelli, M.A. Mayo, and M.D. Summers. 1995. Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of viruses. Springer-Verlag Wien, New York.
  • O’Reilly D.R., L.K. Miller and V.A. Luckow. 1992. Baculovirus expression vectors- A laboratory manual. W. H. Freeman and Company, New York.
  • Pang, Y., J. Yu, L. Wang, X. Hu, W. Bao, G. Li, C. Chen, H. Han, S. Hu, and H.Yang. 2001. Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology 287: 391-404.
  • Passarelli L.A., J.W. Todd and L.K. Miller. 1994. A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases. J. Virol., 68: 4673-4678.
  • Rokas, A. and P.W.H. Holland. 2000. Rare genomic changes as a tool for phylogenetics. Tree 15: 454-459.
  • Snel, B., P. Bork, and M.A. Huynen. 1999. Genome phylogeny based on gene content. Nat. Genet. 21: 108-110.
  • Thiem, S.M. 1997. Prospects for altering host range for baculovirus bionsecticides. Environ. Biotech. 8: 317-322.
  • Toprak, U., Ş. Bayram and M.O. Gürkan. 2005a. Baculoviruses in biological control, do they have a future in Turkey? Çukurova University Agricultural Faculty Journal. In Press.
  • Toprak, U., Ş. Bayram and M.O. Gürkan. 2005b. Comparative Biological Activities of a Plaque Purified and a Wild Strain of SpliNPV-B variant against Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae). Pest Management Science. In Press.
  • Toprak, U., Ş. Bayram and M.O. Gürkan. 2005c. Gross pathology of SpliNPVs and alterations in Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) morphology due to baculoviral infection. Journal of Agricultural Sciences 11(1): 65-71.
  • Toprak, U. and M.O. Gürkan. 2004. First Record of a NPV isolated from Spodoptera littoralis (Boisd.) (Lepidoptera:Noctuidae) in Turkey and Its Molecular Identification according to the partial lef-8 gene. Turkish J. Biol. 28 (2-4): 71-77.
  • Wang L.H., J.X. Yu, X.H. Hu and Y. Pang. 2001. Sequence analysis of the BamHI-J fragment of the Spodoptera litura Multicapsid Nucleopolyhedrovirus. Acta. Bioch. Biop. Sin., 33 (6): 615-620.
  • Wolff J.L.C., L.M. Herzog, L. Sun and D.B. Levin. 1998. Identification and characterization of the Spodoptera littoralis nucleopolyhedrovirus type B lef-3 gene. Arch. Virol. 143: 743-767.
  • Zanotto P.M. de A, B.D. Kessing and J.E. Maruniak. 1993. Phylogenetic Interrelationships among Baculoviruses: Evolutionary Rates and Host Associations. J. Invertebr. Pathol. 62(2): 147-164.

The Partial Sequence Comparison of lef-8 gene Obtained from a Turkish SpliNPV–B with Several Baculoviruses

Yıl 2005, Cilt: 11 Sayı: 04, 384 - 391, 01.11.2005

Umut Toprak İ. Alper Susurluk M. Oktay Gürkan

https://doi.org/10.1501/Tarimbil_0000000553

Öz

Spodoptera littoralis Boisd. Lepidoptera:Noctuidae nucleopolyhedrovirus SpliNPV is a member of Baculoviridae and belongs to the group II NPVs. Lef-8 is a well-conserved gene that is characterized in most of the lepidopteran NPVs. Nucleotide and aminoacid sequences of the encoded proteins and gene content may be phylogenetically informative. In this study, lef-8 gene in the Turkish SpliNPV-B SpliNPV-TR1 genome was partially amplified by PCR and analyzed by amino-acid sequences with several baculoviruses. Amino-acid sequences of partial SpliNPV-TR1 lef-8 gene was 84 % identical to the lef-8 of SpltNPV while revealed it to have about only 57 %, 55 %, 51 %, 50 %, 50 %, 50 % and 49 % of its amino-acid sequence identity with AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV and BufuNPV, respectively. The sequences between SpliNPV-TR1 and granuloviruses also produced low levels of sequence identities maximum 46 % . Although group II NPVs were rather isolated from noctuid hosts, the low level identity values detected between these noctuid NPVs except the high idendity value between SpliNPV-TR1 and SpltNPV revealed that these isolates can not have a monophyletic origin

Anahtar Kelimeler

Baculovirus spodoptera littoralis noctuidae spliNPV-TR1 lef-8 sequence comparison

Kaynakça

  • Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI- BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25: 3389-3402.
  • Bulach D.M., C.A. Kumar, A. Zaia, B. Liang and D.E. Tribe. 1999. Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J. Invertebr. Pathol. 73: 59-73.
  • Chen X., W.F.J. IJkel, R. Tarchini, X. Sun, H. Sandbrink, H. Wang and S. Peters. 2001. The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J. Gen. Virol. 82: 241-257.
  • Cherry, C.L. and M.D. Summers. 1985. Genotypic variation among wild isolates of two nuclear polyhedrosis viruses from Spodoptera littoralis. J. Invertebr. Pathol. 46: 289-295.
  • Christen A.A. and B. Montalbano. 1990. Estimation of quantity of polymerase chain reaction products by ethidium bromide agarose plate assay. Biotechniques 9(3): 310.
  • Croizier G., K. Boukhoudmi-Amiri and L. Croizier. 1989. A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome. Arch. Virol. 104: 145-151.
  • El-Salamouny S., M. Lange, M. Jutzi, J. Huber and J.A. Jehle. 2003. Comparative study on the susceptibility of cutworms (Lepidoptera:Noctuidae) to Agrotis segetum nucleopolyhedrovirus and A. ipsilon nucleopolyhedrovirus. J. Invertebr. Pathol. 84: 75-82.
  • Faktor O. and B. Kamensky. 1997. Genomic localization and nucleotide sequence of a lef-8 gene of the Spodoptera littoralis nucleopolyhedrovirus. Virus genes 15 (1): 9-15.
  • Faktor O., M. Toister-Achituv and O. Nahum. 1997. Enhancer element, repetitive sequences and gene organization in an 8-kbp region containing the polyhedrin gene of the Spodoptera littoralis nucleopolyhedrovirus. Arch. Virol. 142: 1-15.
  • Friesen, P.D. and L.K. Miller. 1986. The regulation of baculovirus gene expression. Eds: pp. 31-50, W. Doerfler. and P. Boehm (eds). The molecular biology of baculoviruses. Springer-Verlag, Berlin,.
  • Guarino, L., J. Jin and W. Dong. 1998. Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase. J. Virol. 72: 10003-10010.
  • Herniou E.A., T. Luque, X. Chen, J.M. Vlak, D. Winstanley, J.S. Cory and D. O’Reilly. 2001. Use of whole genome sequence data to infer Baculovirus pylogeni. J. Virol. 17: 8117-8126.
  • Herniou E.A., J.A. Olezewski, J.S. Cory and D.R. O’Reilly. 2003. The genome sequence and evolution of baculoviruses. Annu. Rev. Entomol. 48: 211-234.
  • Herniou, E.A., J.A. Olezewski, D.R. O’Reilly and J.S. Cory. 2004. Ancient coevolution of baculoviruses and their insect hosts. J. Virol. 78: 3244-3251.
  • Hu Z., R. Broer, J. Westerlaken, J.W.M. Martens, F. Jin, L.M. Wang and J.M. Vlak. 1997. Identification and nucleotide sequence of the ecdysteroid UDP-glucosyltransferase gene of the Buzura suppressaria nucleopolyhedrovirus. Virus Res. 47: 91-97.
  • Huang J. and Levin D.B. 2001. Identification, transcription and sequence analysis of the Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) DNA polymerase gene Arch. Virol. 146: 303-326.
  • Huang, J., X. Liu and Levin D.B. 1999. Characterization of Spodoptera littoralis type B nucleopolyhedrovirus infection in selected insect cell lines. Arch. Virol. 144: 935-955.
  • Ilkel, W. F. J., E. A. Van Strien, J. G. M. Heldens, R. Broer, D. Zuidema, R. W. Goldbach and J. M. Vlak. 1999. Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J. Gen. Virol., 80: 3289-3304.
  • Jehle, J.A. 2004. The mosaic structure of the polyhedrin gene of the Autographa californica nucleopolyhedrovirus (AcMNPV). Virus Genes 29: 5-8.
  • Kislev, N. and M. Edelman. 1982. DNA restriction-pattern differences from geographic isolates of Spodoptera littoralis nuclear polyhedrosis virus. Virology 119: 219-222.
  • Lange, M. and J.A. Jehle. 2003. The genome of the Crptophlebia leucotreta granulovirus. Virology, 317: 220-236.
  • Lange, M., H. Wang, H. Zhihong and J.A. Jehle. 2004. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology 325: 36-47.
  • Levin D.B., M.A. Laitinen, C. Clarke, C.J. Lucarotti, B. Morin and I.S. Otvos. 1997. Characterization of nuclear polyhedrosis viruses from the three subspecies of Lambdina fiscellaria. J. Invertebr. Pathol. 69: 125-134.
  • Levin D.B. and B. Whittome. 2000. Codon usage in nucleopolyviruses. J. Gen. Virol. 81: 2313-2325.
  • Maeda, S., Y. Mukohara, and A. Kondo. 1990. Characteristically distinct isolates of the nuclear polyhedrosis virus from Spodoptera litura. J. Gen. Virol. 71: 2631-2639.
  • Martigoni, M.E. and P.J. Iwai. 1981. A catalogue of viral diseases of insects, mites, and ticks. Eds: pp. 897-911. H. D. Burges, Microbial control of pests and plant diseases 1970-1980. Academic Pres, London
  • Murphy, F.A., C.M. Fauquet, D.H.L. Biship, S.A. Ghabrial, A.W. Jarvis, G.P. Martelli, M.A. Mayo, and M.D. Summers. 1995. Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of viruses. Springer-Verlag Wien, New York.
  • O’Reilly D.R., L.K. Miller and V.A. Luckow. 1992. Baculovirus expression vectors- A laboratory manual. W. H. Freeman and Company, New York.
  • Pang, Y., J. Yu, L. Wang, X. Hu, W. Bao, G. Li, C. Chen, H. Han, S. Hu, and H.Yang. 2001. Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology 287: 391-404.
  • Passarelli L.A., J.W. Todd and L.K. Miller. 1994. A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases. J. Virol., 68: 4673-4678.
  • Rokas, A. and P.W.H. Holland. 2000. Rare genomic changes as a tool for phylogenetics. Tree 15: 454-459.
  • Snel, B., P. Bork, and M.A. Huynen. 1999. Genome phylogeny based on gene content. Nat. Genet. 21: 108-110.
  • Thiem, S.M. 1997. Prospects for altering host range for baculovirus bionsecticides. Environ. Biotech. 8: 317-322.
  • Toprak, U., Ş. Bayram and M.O. Gürkan. 2005a. Baculoviruses in biological control, do they have a future in Turkey? Çukurova University Agricultural Faculty Journal. In Press.
  • Toprak, U., Ş. Bayram and M.O. Gürkan. 2005b. Comparative Biological Activities of a Plaque Purified and a Wild Strain of SpliNPV-B variant against Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae). Pest Management Science. In Press.
  • Toprak, U., Ş. Bayram and M.O. Gürkan. 2005c. Gross pathology of SpliNPVs and alterations in Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) morphology due to baculoviral infection. Journal of Agricultural Sciences 11(1): 65-71.
  • Toprak, U. and M.O. Gürkan. 2004. First Record of a NPV isolated from Spodoptera littoralis (Boisd.) (Lepidoptera:Noctuidae) in Turkey and Its Molecular Identification according to the partial lef-8 gene. Turkish J. Biol. 28 (2-4): 71-77.
  • Wang L.H., J.X. Yu, X.H. Hu and Y. Pang. 2001. Sequence analysis of the BamHI-J fragment of the Spodoptera litura Multicapsid Nucleopolyhedrovirus. Acta. Bioch. Biop. Sin., 33 (6): 615-620.
  • Wolff J.L.C., L.M. Herzog, L. Sun and D.B. Levin. 1998. Identification and characterization of the Spodoptera littoralis nucleopolyhedrovirus type B lef-3 gene. Arch. Virol. 143: 743-767.
  • Zanotto P.M. de A, B.D. Kessing and J.E. Maruniak. 1993. Phylogenetic Interrelationships among Baculoviruses: Evolutionary Rates and Host Associations. J. Invertebr. Pathol. 62(2): 147-164.
Toplam 40 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Research Article
Yazarlar

Umut Toprak Bu kişi benim

İ. Alper Susurluk Bu kişi benim

M. Oktay Gürkan Bu kişi benim

Yayımlanma Tarihi 1 Kasım 2005
Gönderilme Tarihi 1 Kasım 2005
Yayımlandığı Sayı Yıl 2005 Cilt: 11 Sayı: 04

Kaynak Göster

APA Toprak, U., Susurluk, İ. A., & Gürkan, M. O. (2005). The Partial Sequence Comparison of lef-8 gene Obtained from a Turkish SpliNPV–B with Several Baculoviruses. Journal of Agricultural Sciences, 11(04), 384-391. https://doi.org/10.1501/Tarimbil_0000000553

Journal of Agricultural Sciences is published open access journal. All articles are published under the terms of the Creative Commons Attribution License (CC BY).