Alarmins and Related Molecules in Elective Surgery

Strohalmová, Sabina; Levová, Kateřina; Kuběna, Aleš Antonín; Hoskovec, David; Krška, Zdeněk; Zima, Tomáš; Kalousová, Marta

doi:10.14712/fb2023069020050

Folia Biologica > Archive > 2023, Vol. 69 > Issue 2 > Alarmins and Related Molecules in…

Fol. Biol. 2023, 69, 50-58

https://doi.org/10.14712/fb2023069020050

Alarmins and Related Molecules in Elective Surgery

Sabina Strohalmová^1,2, Kateřina Levová², Aleš Antonín Kuběna², David Hoskovec¹, Zdeněk Krška¹, Tomáš Zima², Marta Kalousová²

¹1st Department of Surgery – Department of Abdominal, Thoracic Surgery and Traumatology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
²Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic

Received June 2023
Accepted September 2023

Surgery is associated with alterations of alarmins’ and related molecules’ levels. The aim of this study was to investigate which biomarkers are most involved in surgery. The studied group consisted of 58 patients with inguinal or umbilical hernia or cholecystolithiasis and 21 healthy controls for comparison. We also added seven acute patients with appendicitis, cholecystitis and incarcerated hernia. Serum concentrations of soluble receptor of advanced glycation end-products (sRAGE), extracellular newly identified receptor for advanced glycation end-products binding protein (EN-RAGE), calprotectin, high mobility group box 1 (HMGB1) and interleukin 6 (IL-6) were analysed by ELISA before and after surgery. Preoperative concentrations of calprotectin were significantly decreased while concentrations of sRAGE were significantly increased in patients compared to controls; the concentrations of EN-RAGE and HMGB1 did not differ significantly. IL-6 levels were undetectable in elective patients preoperatively and in controls. Postoperatively, there was a significant increase of EN-RAGE, calprotectin, HMGB1, and IL-6 and a significant decrease of sRAGE compared to preoperative levels. In acute patients, all tested molecules except for sRAGE were significantly increased preoperatively, and sRAGE was significantly decreased. In contrast, after surgery, we could observe a further increase in IL-6; the other biomarkers did not differ significantly. We can conclude that the concentrations of all tested biomarkers are significantly influenced by elective surgery. The postoperative levels of all tested molecules increase except for sRAGE, whose level is significantly decreased after surgery. In acute states, these molecules are already increased, and the influence of surgery is, apart from IL-6, insignificant.

Keywords

sRAGE, EN-RAGE, calprotectin, HMGB1, IL-6, elective surgery.

Funding

The study was supported by research projects funded by the Czech Ministry of Health (MH CZ DRO VFN 64165) and Charles University (Cooperatio Laboratory Diagnostics and Surgical Disciplines – Abdominal Surgery and SVV260630).