Folia Parasitologica 64:018 (2017) | DOI: 10.14411/fp.2017.018

Cryptosporidium meleagridis and C. baileyi (Apicomplexa) in domestic and wild birds in Algeria

Abd Elkarim Laatamna1, Nikola Holubová2,3, Bohumil Sak2, Martin Kváč2,3
1 Faculty of Natural Sciences and Life, University of Djelfa, Moudjbara, Djelfa, Algeria;
2 Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic;
3 Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic

A total of 345 faecal samples were collected from domestic, captive and wild birds in rural areas, urban areas and a Zoo in Algeria. Samples were screened for the presence of parasites belonging to the genus Cryptosporidium Tyzzer, 1910 by microscopy and PCR analysis of the small-subunit rRNA (SSU), actin and 60-kDa glycoprotein (gp60) genes. Cryptosporidium spp. were detected in 31 samples. Sequence analysis of SSU and actin genes revealed the presence of C. baileyi Current, Upton et Haynes, 1986 in domestic chicken broilers (n = 12), captive ostriches (n = 4) and a wild mallard (n = 1), and C. meleagridis Slavin, 1955 in a graylag goose (n = 1), chickens (n = 11) and turkeys (n = 2). Twenty-three chicken and two turkey broilers from five farms were positive for cryptosporidia, with an overall prevalence of 2% and 6%, respectively. Both C. meleagridis and C. baileyi were detected in farmed chicken broilers, with a prevalence ranging from 9% to 69%. Farmed turkeys broilers were positive only for C. meleagridis, with a 13% prevalence at the animal level. Subtyping of C. meleagridis isolates at the gp60 locus showed the presence of subtype IIIgA22G3R1 in graylag goose and chicken broilers and IIIgA23G2R1 in chicken and turkey broilers. Infection with cryptosporidia was not associated with any clinical diseases. The results of the present study, which provides the first data on the prevalence of Cryptosporidium spp. in wild birds in Africa, demonstrate the presence of human pathogenic C. meleagridis in both domestic and wild birds in Algeria.

Keywords: avian cryptosporidia, PCR, epidemiology, Northern Africa

Received: February 23, 2017; Accepted: June 6, 2017; Published online: June 13, 2017  Show citation

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Elkarim Laatamna, A., Holubová, N., Sak, B., & Kváč, M. (2017). Cryptosporidium meleagridis and C. baileyi (Apicomplexa) in domestic and wild birds in Algeria. Folia Parasitologica, 64, Article 2017.018. https://doi.org/10.14411/fp.2017.018
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