Folia Parasitologica 49[3] 221-226 (2002) | DOI: 10.14411/fp.2002.040

Some specific and non-specific phosphatases of the sporocyst of Fasciola hepatica. II. Enzymes associated with the membrane transport

Mirosława Humiczewska
Department of Human Ecology, University of Szczecin, al. Piastów 40 B, 71-065 Szczecin, Poland

Using histochemical and cytophotometric methods, enzymes responsible for the membrane transport (alkaline phosphatase, adenosine triphosphatase, and 5-nucleotidase) in the developing sporocyst of Fasciola hepatica (L., 1758) were studied. The most active metabolism occurred in the germ balls of sporocysts on the 8th and 15th days of development, which is associated with intensive proliferation and subsequently differentiation of embryos within the germ balls.

Keywords: Fasciola hepatica, sporocyst, phosphatases, histochemical and cytophotometric methods

Received: April 17, 1998; Accepted: February 8, 2002; Published: September 1, 2002  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Humiczewska, M. (2002). Some specific and non-specific phosphatases of the sporocyst of Fasciola hepatica. II. Enzymes associated with the membrane transport. Folia Parasitologica49(3), 221-226. doi: 10.14411/fp.2002.040
Download citation

References

  1. ALTMANN F.P. 1971: The use of the recording microdensitometer for the quantitative measurement of enzyme activities inside tissue sections. Histochemistry 27: 125136 Go to original source... Go to PubMed...
  2. BRYANT C. 1994: Ancient biochemistries and the evolution of parasites. Int. J. Parasitol. 24: 1089-1097 Go to original source... Go to PubMed...
  3. CESARI I.M., PUJOL F.H., RODRIGUEZ M., de NOYA B.A. 1991: Antigenic enzymes of Schistosoma mansoni: possible use for immunodiagnosis. Mem. Inst. Oswaldo Cruz 82: 172-177 Go to original source...
  4. CHAYEN J., BITENSKY L., BUTCHER R.G., POULTER R.W. 1969: A Guide to Practical Histochemistry. Oliver and Boyd, Edinburg, 234 pp
  5. CZAPSKI Z. 1977: Biologiczne Aspekty Epidemiologii Fasciolozy. Monografie Akademii Wychowania Fizycznego w Poznaniu. Poznan (Poland), 95 pp
  6. DUM T.S., YOSHINO T.P. 1988: Schistosoma mansoni: the origin and expression of a tegumental surface antigen on the miracidium and primary sporocysts. Exp. Parasitol. 67: 167-181 Go to original source... Go to PubMed...
  7. ERASMUS D.A. 1972: The Biology of Trematodes. Universities Press Belfast, 423 pp
  8. GIBODA M., ZDARSKA Z. 1994: Alkaline phosphatase as marker of Schistosoma mansoni egg viability. Folia Parasitol. 41: 55-58 Go to PubMed...
  9. HUMICZEWSKA M. 1975: Oxidative enzymes in the development of Fasciola hepatica L. III. The activities of oxidases and dehydrogenases in the sporocyst. Folia Histochem. Cytochem. 13: 51-60
  10. HUMICZEWSKA M. 1976: Specific and non-specific phosphatases in the miracidium of Fasciola hepatica L. Folia Histochem. Cytochem. 14: 231-236
  11. HUMICZEWSKA M. 1988: Morphology of the Fasciola hepatica L. sporocyst in intermediate hosts Lymnaea tomentosa and Galba truncatula. Folia Biol. 37: 61-72
  12. HUMICZEWSKA M. 1996: Some specific and non-specific phosphatases in the Fasciola hepatica sporocysts. I. Intracellular digestion enzymes. Zool. Pol. 41: 51-63
  13. KIEREK-JASZCZUK D. 1981: Heterogennosc fosfatazy alkalicznej. Postepy Biochemii 27: 217-221 Go to PubMed...
  14. KOJE M., CHRISTENSEN N.O., NANSEN P. 1976: Stereoscan studies of eggs, free-swimming and penetrating miracidia and early sporocysts of Fasciola hepatica. Z. Parasitenkd. 51: 70-90 Go to original source...
  15. LEWIS S.A., STRAND M. 1991: Characterization of proteins and immunogens released by adult Schistosoma mansoni. J. Parasitol. 77: 263-270 Go to original source... Go to PubMed...
  16. MEULEMAN E.A., HOLZMANN P.J., PEET R.C. 1980: The development of daughter sporocysts inside the mother sporocyst of Schistosoma mansoni with special reference to the ultrastructure of the body wall. Z. Parasitenkd. 61: 201-212 Go to original source... Go to PubMed...
  17. PEARSE A.G.E. 1968: Histochemistry. Churchill Livingstone. Edinburg and London, 684 pp
  18. PUJOL F.H., CESARI I.M. 1990: Antigenicity of adult Schistosoma mansoni alkaline phosphatase. Parasit. Immunol. 12: 189-198 Go to original source... Go to PubMed...
  19. SAWICKA T. 1980: Aktywnosc nukleolityczna plazmolemmy komorek ssakow. Postepy Biologii Komorki 7: 1-7
  20. SMYTH J.D., HALTON D.W. 1983: The Physiology of Trematodes. Edinburg Cambr. Univ. Press. London and New York, 322 pp
  21. SOUTHGATE V.R. 1970: Observations on the tegument of the miracidium and on the formation of the tegument of the sporocyst of Fasciola hepatica. Parasitology 61: 177190 Go to original source... Go to PubMed...
  22. THREADGOLD L.T. 1984: Parasitic Platyhelminthes. In: Biology of the Integument, Vol. 1, Invertebrates. Springer Verlag, Berlin, 297 pp Go to original source...
  23. THREADGOLD L.T., GALLAGHER S.E. 1968: Electron microscope studies of Fasciola hepatica. I. The ultrastructure and interrelationship of the parenchymal cells. Parasitology 56: 299-304 Go to original source... Go to PubMed...
  24. ZDARSKA Z., SOBOLEVA T.N. 1984: Histochemistry of the cercaria and sporocyst of Postharmostomum gallinum (Brachylaimidae) Folia Parasitol. 31: 333-338 Go to PubMed...
  25. ZDARSKA Z., SOBOLEVA T.N., OSIPOVSKAYA L.L. 1984: Histochemical and morphological studies on the sporocyst and metacercaria of Leucochloridium perturbatum. Folia Parasitol. 31: 29-36