Eur. J. Entomol. 115: 192-197, 2018 | DOI: 10.14411/eje.2018.017

Assessing genetic and morphological variation in populations of Eastern European Lucilia sericata (Diptera: Calliphoridae)Original article

Anna V. DIAKOVA1, Dmitry M. SCHEPETOV2, Nadezhda Y. OYUN1,3,4, Anatole I. SHATALKIN5, Tatiana V. GALINSKAYA1,6
1 Entomology Department, Biological Faculty, Lomonosov Moscow State University, Leninskie gory 1-12, Moscow 119234, Russia; e-mails: anndiakova@yandex.ru, nad_oyun@mail.ru, nuha_1313@list.ru
2 National Research University Higher School of Economics, Moscow, Russia; e-mail: schep@mail.ru
3 Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina St. 3, Moscow 119991, Russia
4 Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases, Sechenov University, Malaya Pirogovskaya St. 20-1, Moscow 119435, Russia
5 Zoological Museum, Lomonosov Moscow State University, Bol'shaya Nikitskaya St. 6, Moscow 125009, Russia; e-mail: shatalkin@mail.ru
6 Scientific-Methodological Department of Entomology, All-Russian Plant Quarantine Center, Pogranichnaya 32, Bykovo 140150, Russia

The population structures of different species of Calliphoridae flies are highly diverse at different locations. We investigated populations of the Eastern European L. sericata using chaetotaxy and eight microsatellite loci. Our results strongly indicate that a panmictic population of L. sericata exists in the area studied, possibly with a high rate of intra-population gene flow. Analysis of chaetotaxy also supports the panmictic population hypothesis.

Keywords: Diptera, Calliphoridae, Lucilia sericata, forensic entomology, microsatellite, population genetic structure, blowfly

Received: October 5, 2017; Revised: March 12, 2018; Accepted: March 12, 2018; Published online: May 2, 2018  Show citation

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DIAKOVA, A.V., SCHEPETOV, D.M., OYUN, N.Y., SHATALKIN, A.I., & GALINSKAYA, T.V. (2018). Assessing genetic and morphological variation in populations of Eastern European Lucilia sericata (Diptera: Calliphoridae). EJE115, Article 192-197. https://doi.org/10.14411/eje.2018.017
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References

  1. Anstead C.A., Korhonen P.K., Young N.D., Hall R.S., Jex A.R., Murali S.C., Hughes D.S.T., Lee S.F., Perry T., Stroehlein A.J. et al. 2015: Lucilia cuprina genome unlocks parasitic fly biology to underpin future interventions. - Nature Commun. 6: 7344, 6 pp. Go to original source...
  2. Chakrabarti S., Kambhampati S. & Zurek L. 2010: Assessment of house fly dispersal between rural and urban habitats in Kansas, USA. - J. Kans. Entomol. Soc. 83: 172-188. Go to original source...
  3. Clarke G.M. & McKenzie J.A. 1987: Genetic architecture and adaptation: Quantitative analysis of sheep and refuse tip populations of the Australian sheep blowfly, Lucilia cuprina. - Austral. J. Biol. Sci. 40: 47-56. Go to original source...
  4. Do C., Waples R.S., Peel D., Macbeth G.M., Tillett B.J. & Ovenden J.R. 2014: NeEstimator v2: re-implementation of software for the estimation of contemporary effective population size (Ne) from genetic data. - Mol. Ecol. Resour. 14: 209-214. Go to original source...
  5. Evanno G., Regnaut S. & Goudet J. 2005: Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. - Mol. Ecol. 14: 2611-2620. Go to original source...
  6. Florin A.-B. 2001: Bottlenecks and blowflies speciation, reproduction and morphological variation in Lucilia. In: Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 660. University of Uppsala, 40 pp.
  7. Florin A.-B. & Gyllenstrand N. 2002: Isolation and characterization of polymorphic microsatellite markers in the blowflies Lucilia illustris and Lucilia sericata. - Mol. Ecol. Notes 2: 113-116. Go to original source...
  8. French N., Wall R., Cripps P.J. & Morgan K.L. 1994: Blowfly strike in England and Wales: the relationship between prevalence and farm and management factors. - Med. Vet. Entomol. 8: 51-56. Go to original source...
  9. Galinskaya T.V., Oyun N.Y., Teterina A.A. & Shatalkin A.I. 2016: DNA barcoding of Nothybidae (Diptera). - Orient. Insects 50: 69-83. Go to original source...
  10. Hall M. & Wall R. 1995: Myiasis of humans and domestic animals. - Adv. Parasitol. 35: 257-334. Go to original source...
  11. Hardy O.J., Charbonnel N., Fréville H. & Heuertz M. 2003: Microsatellite allele sizes: A simple test to assess their significance on genetic differentiation. - Genetics 163: 1467-1482. Go to original source...
  12. Hulce D., Li X., Snyder-Leiby T. & Liu C.S.J. 2011: GeneMarker® genotyping software: Tools to increase the statistical power of DNA fragment analysis. - J. Biomol. Techniq. (Suppl.) 22: S35.
  13. Jarne P. & Lagoda P.J.L. 1996: Microsatellites, from molecules to populations and back. - Trends Ecol. Evol. 11: 424-429. Go to original source...
  14. Jordaens K., Sonet G., Braet Y., De Meyer M., Backeljau T., Goovaerts F., Bourguignon L. & Desmyter S. 2013: DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). - ZooKeys 365: 149-174. Go to original source...
  15. Karabey T. & Sert O. 2014: The analysis of pupal development period in Lucilia sericata (Diptera: Calliphoridae) forensically important insect. - Int. J. Legal Med. 40: 47-56. Go to original source...
  16. Li Y.-C., Korol A.B., Fahima T. & Nevo E. 2004: Microsatellites within genes: structure, function, and evolution. - Mol. Biol. Evol. 21: 991-1007. Go to original source...
  17. Lyra M.L., Fresia P., Gama S., Christina J., Klaczko L.B. & Azeredo-Espin A.M.L. 2005: Analysis of mitochondrial DNA variability and genetic structure in populations of New World screwworm flies (Diptera: Calliphoridae) from Uruguay. - J. Med. Entomol. 42: 589-595. Go to original source...
  18. Lyra M.L., Klazsko L.B. & Azeredo-Espin A.M.L. 2009: Complex patterns of genetic variability in populations of the New World screwworm fly revealed by mitochondrial DNA markers. - Med. Vet. Entomol. 23: 32-42. Go to original source...
  19. Martins W.S., Lucas D.C.S., Neves K.F. de S. & Bertioli D.J. 2009: WebSat - a web software for microsatellite marker development. - Bioinformation 3: 282-283. Go to original source...
  20. Nelson W.A. & Rice D.A. 1956: Human myiasis caused by the greenbottle fly, Phaenicia sericata (Meigen). - Can. Med. Assoc. J. 75: 839.
  21. Picard C.J. & Wells J.D. 2009: Survey of the genetic diversity of Phormia regina Meigen (Diptera: Calliphoridae) using amplified fragment length polymorphisms (AFLP). - J. Med. Entomol. 46: 664-670. Go to original source...
  22. Picard C.J. & Wells J.D. 2010: The population genetic structure of North American Lucilia sericata (Diptera: Calliphoridae), and the utility of genetic assignment methods for reconstruction of postmortem corpse relocation. - Forens. Sci. Int. 195: 63-67. Go to original source...
  23. Pritchard J.K., Stephens M. & Donnelly P. 2000: Inference of population structure using multilocus genotype data. - Genetics 155: 945-959. Go to original source...
  24. Rasic G. & Keyghobadi N. 2012: The pitcher plant flesh fly exhibits a mixture of patchy and metapopulation attributes. - J. Hered. 103: 703-710. Go to original source...
  25. Rodrigues R.A., Azeredo-Espin A.M.L. & Torres T.T. 2009: Microsatellite markers for population genetic studies of the blowfly Chrysomya putoria (Diptera: Calliphoridae). - Mem. Inst. Oswaldo Cruz 104: 1047-1050. Go to original source...
  26. Stevens J. & Wall R. 1997: Genetic variation in populations of the blowflies Lucilia cuprina and Lucilia sericata (Diptera: Calliphoridae). Random amplified polymorphic DNA analysis and mitochondrial DNA sequences. - Biochem. Syst. Ecol. 25: 81-97. Go to original source...
  27. Torres T.T. & Azeredo-Espin A.M.L. 2005: Development of new polymorphic microsatellite markers for the New World screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae). - Mol. Ecol. Notes 5: 815-817. Go to original source...
  28. Torres T.T. & Azeredo-Espin A.M.L. 2009: Population genetics of New World screwworm from the Caribbean: Insights from microsatellite data. - Med. Vet. Entomol. (Suppl. 1) 23: 23-31. Go to original source...
  29. Torres T.T., Brondani R.P.V, Garcia J.E. & Azeredo-Espin A.M.L. 2004: Isolation and characterization of microsatellite markers in the new world screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae). - Mol. Ecol. Notes 4: 182-184. Go to original source...
  30. Torres T.T., Lyra M.L., Fresia P. & Azeredo-Espin A.M.L. 2007: Assessing genetic variation in New World screwworm Cochliomyia hominivorax populations from Uruguay. In Vreysen M.J.B., Robinson A.S. & Hendrichs J. (eds): Area-Wide Control of Insect Pests: From Research to Field Implementation. Springer, Dordrecht, pp. 183-191. Go to original source...
  31. Waples R.S., Antao T. & Luikart G. 2014: Effects of overlapping generations on linkage disequilibrium estimates of effective population size. - Genetics 197: 769-780. Go to original source...
  32. Williams K.A. & Villet M.H. 2014: Morphological identification of Lucilia sericata, Lucilia cuprina and their hybrids (Diptera, Calliphoridae). - ZooKeys 420: 69-85. Go to original source...

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