Eur. J. Entomol. 113: 446-455, 2016 | DOI: 10.14411/eje.2016.058

Genetic identification of a non-native species introgression into wild population of the field cricket Gryllus campestris (Orthoptera: Gryllidae) in Central EuropeOriginal article

Hanna PANAGIOTOPOULOU1,2, Mateusz BACA3, Katarzyna BACA4, Paweł SIENKIEWICZ5, Piotr ŚLIPIŃSKI1, Michał ŻMIHORSKI6,7,*
1 Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679 Warsaw, Poland; e-mails: hpana@miiz.waw.pl, piotrs@miiz.waw.pl
2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
3 Center for Precolumbian Studies, University of Warsaw, Krakowskie Przedmieście 26/28, 00-927 Warsaw, Poland; e-mail: bacamat@gmail.com
4 Institute of Genetics and Biotechnology, Department of Biology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland; e-mail: katarzyna.ewa.pajak@gmail.com
5 Department of Entomology and Environmental Protection, Poznań University of Life Sciences, Dąbrowskiego 159, 60-594 Poznań, Poland; e-mail: ophonus@gmail.com
6 Institute of Nature Conservation, Polish Academy of Sciences, Mickiewicza 33, 31-120 Kraków, Poland; e-mail: michal.zmihorski@gmail.com
7 Department of Ecology, Swedish University of Agricultural Sciences, Box 7044, SE 750 07 Uppsala, Sweden

Two species of the genus Gryllus occur in Europe: G. campestris and G. bimaculatus. The first is widely distributed in the north-western Palaearctic, while the second, G. bimaculatus, occurs predominantly in the Mediterranean area. There is a visible pattern in the distribution of G. campestris, the insect being rare and threatened in the western part of its range, whereas it is still abundant in the east. Despite the fact that this species is commonly used in laboratory experiments, its natural populations are poorly characterised. In the present study, we analysed cricket populations from the lower Oder and Vistula River valleys in Poland. Based on the phylogeny of the mtDNA cytochrome b fragment, we found that 17% of the individuals studied had a G. bimaculatus-like mtDNA haplotype. Analyses of 11 autosomal microsatellite loci failed to reveal any clear genetic differentiation between individuals assigned to these two clades. This suggests, along with the spatial distribution of G. bimaculatus-like haplotypes, successful interbreeding of G. bimaculatus with native populations of G. campestris. However, both the nuclear data and additional analyses of two X-chromosome-linked microsatellite loci revealed incomplete introgression. Human-mediated introgression seems to be the most plausible explanation of the observed genotypic pattern such that caution needs to be taken in conservation efforts carried out in the western part of the species' range.

Keywords: Orthoptera, Gryllidae, Gryllus campestris, Gryllus bimaculatus, conservation, mtDNA, microsatellite loci, X-chromosome-linked markers

Received: November 9, 2015; Revised: May 25, 2016; Accepted: May 25, 2016; Published online: August 29, 2016  Show citation

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PANAGIOTOPOULOU, H., BACA, M., BACA, K., SIENKIEWICZ, P., ŚLIPIŃSKI, P., & ŻMIHORSKI, M. (2016). Genetic identification of a non-native species introgression into wild population of the field cricket Gryllus campestris (Orthoptera: Gryllidae) in Central Europe. EJE, 113, Article 446-455. https://doi.org/10.14411/eje.2016.058
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