Abstract
An enzyme KfoG with unknown function is coded by the gene kfoG. Gene kfoG belongs to genes from region 2, which are responsible for structure of capsular polysaccharide. Only two enzymes, KfoG and KfoC, coded by genes from region 2, have a glycosyltransferase motif. KfoC is the bifunctional enzyme, which is able to add both GalNAc and GlcUA on nascent polysaccharide, termed chondroitin polymerase. KfoG was predicted to be a fructosyltransferase. The gene that codes the KfoG enzyme was disrupted using homological recombination and absence of this gene was confirmed on both DNA and RNA levels. After disruption no structural changes have been observed, what indicates that fructose branching of the chondroitin backbone is not caused by enzymes, which are coded by genes from region 2 of the K4 capsular gene cluster.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Roberts, I. S. (1996) The biochemistry and genetics of capsular polysaccharide production in bacteria. Annu. Rev. Microbiol. 50, 285–315.
DeAngelis, L. P. and Padgett-McCue, A. J. (2000) Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F. J. Biol. Chem. 275, 24124–24129.
Ninomiya, T., Sugiura, N., Tawada, A., Sugimoto, K., Watanabe, H., and Kimata, K. (2002) Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4. J. Biol. Chem. 277, 21567–21575.
Griffiths, G., Cook, N. J., Gottfridson, E., Lind, T., and Roberts, I. S. (1998) Characterization of the glycosyltransferase enzyme from the Escherichia coli K5 capsule gene cluster and identification and characterization of the glucuronyl active site. J. Biol. Chem. 273, 11752–11757.
Hodson, N., Griffiths, G., Cook, N. J., et al. (2000) Identification that KfiA, a protein essential for the biosynthesis of the Escherichia coli K5 capsular polysaccharide, is an alpha -UDP-GlcNAc glycosyltransferase. The formation of a membrane-associated K5 biosynthetic complex requires KfiA, KfiB, and KfiC. J. Biol. Chem. 275, 27311–27315.
Ubben, D. and Schmitt, R. (1987) A transposable promoter and transposable promoter probes derived from Tn1721. Gene 53, 127–134.
Ausubel, F. M., Brent, R., Kingston, R. E., et al. (eds) (1987) Current Protocols in Molecular Biology. John Wiley & Sons, New York.
Lidholt, K. and Fjelstad, M. (1997) Biosynthesis of the Escherichia coli K4 capsule polysaccharide. A parallel system for studies of glycosyltransferases in chondroitin formation. J. Biol. Chem. 272, 2682–2687.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Krahulec, J., Krahulcová, J., Medová, M. et al. Influence of KfoG on capsular polysaccharide structure in Escherichia coli K4 strain. Mol Biotechnol 30, 129–134 (2005). https://doi.org/10.1385/MB:30:2:129
Issue Date:
DOI: https://doi.org/10.1385/MB:30:2:129