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An antibody-lectin sandwich assay for quantifying protein glycoforms

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Abstract

We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein’s glycoforms by simply substituting the antibody and lectin with specific alternatives,

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Authors and Affiliations

  1. School of Biology and Biochemistry, The Queen’ University, Medical Biology Centre, BT9 BL, Belfast, UK-Northern Ireland

    G. Brian Wisdom

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  1. Fionnuala T. Lundy
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  2. G. Brian Wisdom
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Correspondence to G. Brian Wisdom.

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Lundy, F.T., Wisdom, G.B. An antibody-lectin sandwich assay for quantifying protein glycoforms. Mol Biotechnol 12, 203–206 (1999). https://doi.org/10.1385/MB:12:2:203

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