Skip to main content
SpringerLink
Log in

Analyses for susceptibility of rat anterior pituitary cells to prolactin-releasing peptide

  • Published:
Endocrine Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

We validated the effect of prolactin-releasing peptide (PrRP) on prolactin (PRL) secretion from rat anterior pituitary cells in in vitro culture. We found that culture conditions considerably influenced the response of the anterior pituitary cells to PrRP. Longer culture term (4 d) was required to obtain better responses of the anterior pituitary cells to PrRP in comparison to thyrotropin-releasing hormone (TRH). Under the culture conditions employed here, PrRP was comparable to TRH in the potency promoting PRL secretion, and the action of PrRP was very specific for PRL secretion. The susceptibility of the anterior pituitary cells to PrRP varied in female rats depending on the process of reproduction: the cells prepared from lactating rats were the most sensitive to PrRP compared with those from random-cycle and pregnant rats. Because the expression levels of PrRP receptor mRNA in the pituitary varied during the reproductive process, we speculated that the susceptibility of the anterior pituitary cells would reflect cellular changes including the expression level of PrRP receptors. In addition, treatment with estrogen in vivo enhanced the susceptibility of the cultured anterior pituitary cells in male rats. Our results indicate that the susceptibility of the rat anterior pituitary cells to PrRP is regulated by physiological mechanisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lamberts, S. W. J. and MacLeod, R. M. (1990). Physiol. Rev. 70, 279–318.

    PubMed  CAS  Google Scholar 

  2. Samson, W. K., Martin, L., Mogg, R. J., and Fulton, R. J. (1990). Endocrinology 126, 1610–1617.

    PubMed  CAS  Google Scholar 

  3. Allen, D. L., Low, M. J., Allen, R. G., and Ben-Jonathan, N. (1995). Endocrinology 136, 3093–3099.

    Article  PubMed  CAS  Google Scholar 

  4. Riskind, P. N., Millard, W. J., and Martin, J. B. (1984). Endocrinology 115, 312–316.

    PubMed  CAS  Google Scholar 

  5. Laudon, M., Grossman, D. A., and Ben-Jonathan, N. (1990). Endocrinology 126, 3185–3192.

    PubMed  CAS  Google Scholar 

  6. Hinuma, S., Onda, H., and Fujino, M. (1999). J. Mol. Med. 77, 495–504.

    Article  PubMed  CAS  Google Scholar 

  7. Hinuma, S., Habata, Y., Fujii, R., Kawamata, Y., Hosoya, M., Fukusumi, S., Kitada, C., Masuo, Y., Asano, T., Matsumoto, H., Sekiguchi, M., Kurokawa, T., Nishimura, O., Onda, H., and Fujino, M. (1998). Nature 393, 272–276.

    Article  PubMed  CAS  Google Scholar 

  8. Welch, S. K., O'Hara, B. F., Kilduff, T. S., and Heller, H. C. (1995). Biochem. Biophys. Res. Commun. 209, 606–613.

    Article  PubMed  CAS  Google Scholar 

  9. Fujii, R., Fukusumi, S., Hosoya, M., Kawamata, Y., Habata, Y., Hinuma, S., Sekiguchi, M., Kitada, C., Kurokawa, T., Nishimura, O., Onda, H., Sumino, Y., and Fujino, M. (1999). Regul. Pept. 83, 1–10.

    Article  PubMed  CAS  Google Scholar 

  10. Samson, W. K., Resch, Z. T., Murphy, T. C., and Chang, J.-K. (1998). Endocrine 9, 289–291.

    Article  PubMed  CAS  Google Scholar 

  11. Vale, W., Grant, G., Amoss, M., Blackwell, R., and Guillemin, R. (1972). Endocrinology 91, 562–572.

    PubMed  CAS  Google Scholar 

  12. Shiota, K., Yoshida, K., Noguchi, C., and Nakayama, R. (1984). Acta Endocrinol. (Copenh) 106, 71–78.

    CAS  Google Scholar 

  13. Shiota, K., Yoshida, K., Masaki, T., Kawase, M., Nakayama, R., and Sudo, K. (1984). Endocrinol. Japan 31, 165–175.

    CAS  Google Scholar 

  14. Willoughby, J. O., Pederick, H. J., Jervois, P. M., and Menadue, M. F. (1983). J. Endocr. 99, 477–483.

    Article  PubMed  CAS  Google Scholar 

  15. Shull, J. D., Walent, J. H., and Gorski, J. (1987). J. Steroid Biochem. 26, 451–456.

    Article  PubMed  CAS  Google Scholar 

  16. Gershengorn, M. C., Marcus-Samuels, B. E., and Geras, E. (1979). Endocrinology 105, 171–176.

    Article  PubMed  CAS  Google Scholar 

  17. Baker, B. L., Reel, J. R., Van Dewark, S. D., and Yu Y.-Y. (1974). Anat. Rec. 179, 93–106.

    Article  PubMed  CAS  Google Scholar 

  18. Hymer, W. C., Evans, W. H., Kraicer, J., Mastro, A., Davis, J., and Griswold, E. (1973). Endocrinology 92, 275–287.

    PubMed  CAS  Google Scholar 

  19. Nakano, H., Fawcett, C. P., and McCann, S. M. (1976). Endocrinology 98, 278–288.

    Article  PubMed  CAS  Google Scholar 

  20. Nagy, G. M. and Frawley, L. S. (1990). Endocrinology 127, 2079–2084.

    Article  PubMed  CAS  Google Scholar 

  21. Hill, J. B., Ramsdell, J. S., and Frawley, L. S. (1992). Endocr. Regul. 26, 59–65.

    PubMed  CAS  Google Scholar 

  22. Matsumoto, H., Noguchi, J., Horikoshi, Y., Kawamata, Y., Kitada, C., Hinuma, S., Onda, H., Nishimura, O., and Fujino, M. (1999). Biochem. Biophys. Res. Commun. 259, 321–324.

    Article  PubMed  CAS  Google Scholar 

  23. Tokita, R., Minami, S., Nakata, T., Onodera, H., Imajoh, J., Ono, M., and Miki, N. (1999). Folia Endocrinologica Japonica 75, 125.

    Google Scholar 

  24. Matsumoto, H., Murakami, Y., Horikoshi, Y., Noguchi, J., Habata, Y., Kitada, C., Hinuma, S., Onda, H., and Fujino, M. (1999). Biochem. Biophys. Res. Commun. 257, 264–268.

    Article  PubMed  CAS  Google Scholar 

  25. Chen, C.-T., Dun, S. L., Dun, N. J., and Chang, J.-K. (1999). Brain Res. 822, 276–279.

    Article  PubMed  CAS  Google Scholar 

  26. Iijima, N., Kataoka, Y., Kakihara, K., Bamba, H., Tamada, Y., Hayashi, S., Matsuda, T., Tanaka, M., Honjyo, H., Hosoya, M., Hinuma, S., and Ibata, Y. (1999). NeuroReport 10, 1713–1716.

    Article  PubMed  CAS  Google Scholar 

  27. Maruyama, M., Matsumoto, H., Fujiwara, K., Kitada, C., Hinuma, S., Onda, H., Fujino, M., and Inoue, K. (1999). Endocrinology 140, 2326–2333.

    Article  PubMed  CAS  Google Scholar 

  28. Nishimura, O., Moriya, T., Suenaga, M., Tanaka, Y., Itoh, T., Koyama, N., Fujii, R., Hinuma, S., Kitada, C., and Fujino, M. (1998). Chem. Pharm. Bull. 46, 1490–1492.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Discovery Research Laboratories 1, Pharmaceutical Discovery Research Division, Takeda Chemical Industries Ltd, 10 Wadai, 300-4293, Tsukuba, Ibaraki, Japan

    Yuji Kawamata, Ryo Fujii, Shoji Fukusumi, Yugo Habata, Masaki Hosoya,  Shuji Hinuma, Chieko Kitada, Haruo Onda, Osamu Nishimura & Masahiko Fujino

Authors
  1. Yuji Kawamata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ryo Fujii
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shoji Fukusumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yugo Habata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masaki Hosoya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shuji Hinuma
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chieko Kitada
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Haruo Onda
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Osamu Nishimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Masahiko Fujino
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shuji Hinuma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawamata, Y., Fujii, R., Fukusumi, S. et al. Analyses for susceptibility of rat anterior pituitary cells to prolactin-releasing peptide. Endocr 12, 215–221 (2000). https://doi.org/10.1385/ENDO:12:3:215

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:12:3:215

Key Words

Search

Navigation