Abstract
In adult male rats, serum luteinizing hormone (LH) rises within a few hours of castration. By contrast, in adult female rats, serum LH does not increase reliably until 4–6 d after ovariectomy. The release of gonadotropin-releasing hormone (GnRH) declines in female rats postovariectomy, suggesting an increase in inhibition of the release of GnRH. We investigated whether differences in γ-aminobutyric acid (GABA)-ergic transmission, which inhibits GnRH release, accounts for the sex difference in the response of serum LH to gonadectomy. We examined the effects of GABA-A receptor antagonist bicuculline methiodide (BMI), GABA-B receptor antagonist phaclofen, and transaminase inhibitor aminooxyacetic acid (AOAA), injected subcutaneously, on the postgonadectomy rise in LH. AOAA prevented the postcastration rise in male rats (p<0.05). Female rats treated with BMI, phaclofen, or both BMI and phaclofen (p<0.05) showed a significant increase in LH postovariectomy. BMI had no effect in male rats. GnRH antagonist blocked the BMI-induced increase in serum LH. We conclude that the delay in the rise of serum LH in female rats postovariectomy is at least partly owing to GABAergic inhibition of the release of GnRH.
Similar content being viewed by others
References
Gay, V. and Midgley, A. (1969). Endocrinology 84, 1359–1364.
Luderer, U. and Schwartz, N. B. (1991). Biol. Reprod. 45, 918–926.
Leipheimer, R. E. and Gallo, R. V. (1983). Neuroendocrinology 37, 421–426.
Luderer, U. and Schwartz, N. B. (1994). J. Reprod. Fertil. 100, 613–621.
Clayton, R. N. and Catt, K. J. (1981). Endocrinology 108, 887–891.
Kaiser, U. B., Jakubowiak, A., Stenberger, A., and Chin, W. W. (1993). Endocrinology 133, 931–934.
Fallest, P. C., Hiatt, E. S., and Schwartz, N. B. (1989). Endocrinology 124, 1370–1379.
Elskus, A. A., Phelps, A. F., and Schwartz, N. B. (1995). Neuroendocrinology 61, 301–309.
Hood, S. C. H. and Schwartz, N. B. (1998). Role of GABA in the sex difference in serum LJ post-gonadectomy. Soc. Neurosci. Abstr. 24, 620.
Levine, J. E. and Ramirez, V. D. (1982). Endocrinology 111, 1439–1448.
Dluzen, D. E. and Ramirez, V. D. (1986). Neuroendocrinology 43, 459–465.
Karahalios, D. G. and Levine, J. E. (1988). Neuroendocrinology 47, 504–510.
Levine, J. E. and Duffy, M. T. (1988). Endocrinology 122, 2211–2221.
Phelps, C. P., Kalra, S. P., and Kalra, P. S. (1992). Brain Res. 569, 159–163.
Grattan, D. R., Park, S. K., and Selmanoff, M. (1995). Am. J. Physiol. 268, E685-E692.
Grattan, D. R. and Selmanoff, M. (1994). Neuroendocrinology 59, 141–149.
Grattan, D. R. and Selmanoff, M. (1993). J. Neurochem. 60, 2254–2264.
Grattan, D. R., Rocca, M. S., Sagrillo, C. A., McCarthy, M. M., and Selmanoff, M. (1996). Endocrinology 137, 1–7.
Grattan, D. R. and Selmanoff, M. (1994). J. Endocrinol. 143, 165–174.
Donoso, A. O., Seltzer, A. M., Navorro, C. E., Cabrera, R. J., Lopez, F. J., and Negro-Vilar, A. (1994). Brazil. J. Med. Biol. Res. 27, 921–932.
Seltzer, A. M. and Donoso, A. O. (1992). Neuroendocrinology 55, 28–34.
Ondo, J., Mansky, T., and Wuttke, W. (1982). Exp. Brain Res. 46, 69–72.
Demling, J., Fuchs, E., Baumert, M., and Wuttke, W. (1985). Neuroendocrinology 41, 212–218.
Pong, S. F. and Graham, L. T. (1972). Brain Res. 42, 486–490.
Etchegoyen, G. S. and Zotto, H. D. (1996). Arch. Physiol. Biochem. 104, 287–292.
McCarthy, M. M. (1995). Horm. Behav. 29, 131–140.
Hartman, R. D., He, J.-R., and Barraclough, C. B. (1990). Endocrinology 127, 1336–1345.
Summerville, J. and Schwartz, N. B. (1981). Endocrinology 109, 1442–1447.
Schwartz, N. B. (1995). Can. J. Physiol. Pharmacol. 73, 675–684.
Grady, R. R., Shin, L., Charlesworth, M. C., Cohen-Becker, I. R., Smith, M., Rivier, C., Rivier, J., Vale, W., and Schwartz, N. B. (1985). Neuroendocrinology 40, 246–252.
Froestl, W., Mickel, S. J., Sprecher, G. V., et al. (1995). J. Med. Chem. 38, 3313–3331.
Leranth, C., MacLusky, N. J., Sakamoto, H., Shanabrough, M., and Naftolin, F. (1985). Neuroendocrinology 40, 536–539.
Petersen, S. L., McCrone, S., Coy, D., Adelman, J. P., and Mahan, L. C. (1993). Endocr. J. 1, 29–34.
Leonhardt, S., Seong, J. Y., Kim, K., Thorun, Y., Wuttke, W., and Jarry, H. (1995). Neuroendocrinology 61, 655–662.
Li, S. and Pelletier, G. (1993). Neuroendocrinology 58, 136–139.
Fuchs, E., Mansky, T., Stock, K.-W., Vijayan, E., and Wuttke, W. (1984). Neuroendocrinology 38, 484–489.
Herbison, A. E. (1998). Endocr. Rev. 19, 302–330.
Funabashi, T., Jinnai, K., and Kimura, F. (1997). Neuroreport 8, 771–774.
Boue-Grabot, E., Dufy, B., and Garret, M. (1995). Brain Res. 704, 125–129.
Virmani, M. A., Stojilkovic, S. S., and Catt, K. J. (1990). Endocrinology 126, 2499–2505.
Lux-Lantos, V., Rey, E., and Libertun, C. (1992). Neuroendocrinology 56, 687–693.
Masotto, C. and Negro-Vilar, A. (1987). Endocrinology 121, 2251–2255.
Donoso, A. O. and Banzan, A. M. (1984). Acta Endocrinol. 106, 298–304.
Cruz-Morales, S. E., Quirarte, G. L., Diaz del Guante, M. A., and Prado-Alcala, R. A. (1993). Life Sci. 53, 1325–1330.
Yonkov, D. I. and Georgiev, V. P. (1985). Acta Physiol. Pharm. Bulgarica 11, 44–49.
Herbison, A. E., Chapman, C., and Dyer, R. G. (1991). Exp. Brain Res. 87, 345–352.
Flugge, G., Oertel, W. H., and Wuttke, W. (1986). Neuroendocrinology 43, 1–5.
Grattan, D. R., Rocca, M. S., Straus, K. I., Sagrillo, C. A., Selmanoff, M., and McCarthy, M. M. (1996). Brain Res. 733, 46–55.
Adler, B. A. and Crowley, W. R. (1986). Endocrinology 118, 91–97.
Kang, S. H., Seong, J. Y., Cho, S., Cho, H., and Kim, K. (1995). Neuroendocrinology 61, 486–492.
McCarthy, M. M., Kaufman, L. C., Brooks, P. J., Pfaff, D. W., and Schwartz-Giblin, S. (1995). J. Comp. Neurol. 360, 685–697.
Moguilevsky, J. A., Carbone, S., Szwarcfarb, B., and Rondina, D. (1991). Brain Res. 563, 12–16.
Pericic, D. and Bujas, M. (1997). Exp. Brain Res. 115, 187–190.
Shafizadeh, M., Semnaninan, S., Zarrindast, M. R., and Hashemi, B. (1997). Gen. Pharm. 28, 611–615.
Carmona, E., Gomes, C., and Trelin, G. (1980). Arch. Pharmacol. 312, 51–55.
McAndrews, J. M., Ringstrom, S. J., Dahl, K. D., and Schwartz, N. B. (1994). Endocrinology 134, 158–163.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hood, S.C.H., Schwartz, N.B. Sex difference in serum luteinizing hormone postgonadectomy in the rat. Endocr 12, 35–40 (2000). https://doi.org/10.1385/ENDO:12:1:35
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ENDO:12:1:35