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Priming with magnesium-deficient media inhibits preadipocyte differentiation via potential upregulation of tumor necrosis factor-α

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Abstract

The effect of priming stromal-vascular cells in primary cultures with magnesium-deficient (MgD) media on preadipocyte differentiation was studied. Cultures were derived from dorsal subcutaneous fat tissue of young pigs and maintained 3 d in serum-free control or MgD Dulbecco’s modified Eagle’s medium, 3 d in 10% fetal bovine serum and dexamethasone, and 6 d in insulin. At d 12 of culture, immunocytochemical and glycerophosphate dehydrogenase assays indicated depressed adipocyte differentiation in the MgD groups. Cultures were enriched for preadipocytes up to 50% of total cells. On the third day of treatment with control and MgD medium, total cell lysates were isolated and 50 µg of them were run on two-dimensional gel electrophoresis. The separated proteins from both treatment groups showed similar patterns. However, spots of proteins with predicted molecular weight in the range of 25.8–37.4 kDa and pI of 5.39–5.85 were sixfold denser from the MgD 10 groups than from the controls. These proteins migrate similarly to tumor necrosis factor-α (TNF-α). The amount of TNF-α in cell lysates from the MgD group was about 2.35 times greater than controls determined by TNF-α-ELISA. It is likely that proteins upregulated by MgD medium are TNF-α isoforms.

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Authors and Affiliations

  1. Department of Foods and Nutrition, Faculty of Consumer Sciences, Dawson Hall, University of Georgia, 30602, Athens, GA

    Yourka D. Tchoukalova, Arthur Grider, Michael F. Mouat & Gary J. Hausman

  2. Department of Animal Physiology, Russell Research Center, USDA-ARS, P.O. Box 5677, 30613, Athens, GA

    Gary J. Hausman

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  1. Yourka D. Tchoukalova
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Tchoukalova, Y.D., Grider, A., Mouat, M.F. et al. Priming with magnesium-deficient media inhibits preadipocyte differentiation via potential upregulation of tumor necrosis factor-α. Biol Trace Elem Res 74, 11–21 (2000). https://doi.org/10.1385/BTER:74:1:11

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  • DOI: https://doi.org/10.1385/BTER:74:1:11

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