Abstract
A thermostable d-hydantoinase was isolated from thermophilic Bacillus thermocatenulatus GH-2 and purified to homogeneity by using immunoaffinity chromatography. The molecular mass of the enzyme was determined to be about 230 kDa, and a value of 56 kDa was obtained as a molecular mass of the subunit on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, implying that oligomeric structure of the enzyme is tetrameric. Isoelectric pH of the enzyme was found to be approx 4.3. The enzyme required Mn2+ for the activity and exhibited its highest activity with phenylhydantoin as a substrate. The optimal pH and temperature for catalytic activity were about 7.5 and 65°C, respectively. The half-life of the enzyme was estimated to be about 45 min at 80°C.
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Park, JH., Kim, GJ., Lee, SG. et al. Purification and characterization of thermostable d-hydantoinase from Bacillus thermocatenulatus GH-2. Appl Biochem Biotechnol 81, 53–65 (1999). https://doi.org/10.1385/ABAB:81:1:53
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DOI: https://doi.org/10.1385/ABAB:81:1:53