Abstract
Candida rugosa lipase was entrapped in silica sol-gel particles prepared by hydrolysis of methyltrimethoxysilane and assayed by p-nitrophenyl palmitate hydrolysis, as a function of pH and temperature, giving pH optima of 7.8 (free enzyme) and 5.0–8.0 (immobilized enzyme). The optimum temperature for the immobilized enzyme (50–55°C) was 19°C higher than for the free enzyme. Thermal, operational, and storage stability were determined with n-butanol and bytyric acid, giving at 45°C a half-life 2.7 times greater for the immobilized enzyme; storage time was 21 d at room temperature. For ester synthesis, the optimum temperature was 47°C, and high esterification conversions were obtained under repeated batch cycles (half-life of 138 h).
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Soares, C.M.F., de Castro, H.F., Itako, J.E. et al. Characterization of Sol-gel bioencapsulates for ester hydrolysis and synthesis. Appl Biochem Biotechnol 123, 845–859 (2005). https://doi.org/10.1385/ABAB:123:1-3:0845
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DOI: https://doi.org/10.1385/ABAB:123:1-3:0845