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Protective effects of ascorbic acid, Dl-α-tocopherol acetate, and sodium selenate on ethanol-induced gastric mucosal injury of rats

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Abstract

In this study, the effect of ascorbic acid (vitamin C), Dl-α-tocopherol acetate (vitamin E), and sodium selenate (selenium) on ethanol-induced gastric mucosal injury in rats was investigated morphologically and biochemically. The gastric mucosal injury was produced by administration of 1 mL of absolute ethanol to each rat. Animals received vitamin C (250 mg/kg), vitamin E (250 mg/kg), and selenium (0.5 mg/kg) for 3 d 1 h prior to the administration of absolute ethanol. In gastric mucosa of rats given ethanol according to control groups, neuronal nitric oxide expression decreased. This immunoreactivity was much lower in the group given ethanol+vitamin C+vitamin E+selenium than the control group and the ethanol-induced group. Scanning electron microscopic evaluation of the ethanol-induced group, when compared to control groups, revealed degenerative changes in gastric mucosa, whereas a good arrangement in surface topography of gastric mucosa in the group given ethanol + vitamin C+vitamin E + selenium was observed. In the group administered ethanol, a reduction of the stomach glutathione (GSH) and serum total protein levels and increases in serum sialic acid, triglycerides, and stomach lipid peroxidation (LPO) levels were observed. Vitamin C+vitamin E+Se administration to alcohol-treated rats significantly increased the serum total protein, triglyceride levels, and stomach GSH levels and significantly lowered the levels of serum sialic acid and stomach LPO compared to untreated alcohol-supplemented rats. As a result of these findings, we can say that the combination of vitamin C, vitamin E, and selenium has a protective effect on ethanol-induced gastric mucosal injury of rats.

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Authors and Affiliations

  1. Department of Internal Medicine, Istanbul Medical Faculty, Istanbul University, 34390, Capa, Turkey

    Sadakat Ozdil

  2. Department of Chemistry, Faculty of Engineering, Istanbul University, 34850, Avcılar, Turkey

    Refiye Yanardag

  3. Department of Histology and Embryology, Faculty of Medicine, Kadir Has University, 80810, Gayrettepe, Turkey

    Meral Koyuturk

  4. Department of Biology, Faculty of Science, Istanbul University, 34459, Vezneciler, Turkey

    Sehnaz Bolkent

  5. Department of Histology and Embryology, Marmara Medical Faculty, Marmara University, 81326, Haydarpasa, Istanbul, Turkey

    Serap Arbak

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  1. Sadakat Ozdil
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Ozdil, S., Yanardag, R., Koyuturk, M. et al. Protective effects of ascorbic acid, Dl-α-tocopherol acetate, and sodium selenate on ethanol-induced gastric mucosal injury of rats. Biol Trace Elem Res 99, 173–189 (2004). https://doi.org/10.1385/BTER:99:1-3:173

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