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Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chickens

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Abstract

This experiment was conducted to evaluate the effects of zinc (ZnSO4H2O) and vitamin A (retinol) supplementation on performance, carcass characteristics, and serum concentrations of glucose, cholesterol, total protein, and malondialdehyde (MDA) as an indicator of lipid peroxidation in broiler chickens (Ross) reared at a high temperature (34°C). One hundred twenty 10-d-old male broilers were randomly assigned to 4 treatment groups, 3 replicates of 10 birds each. The birds were fed either a basal diet or the basal diet supplemented with either 30 mg Zn/kg diet, 4.5 mg (15,000 IU) retinol/kg diet, or 30 mg Zn+4.5 mg retinol/kg diet. Supplemental zinc and vitamin A significantly increased live weight gain and improved feed efficiency (p<0.05). However, a combination of zinc and vitamin A, rather than each separately, provided a greater performance. Hot and chilled carcass weights and yields and the weights of internal organs with the exception of abdominal fat were greater for each supplement (p<0.05) compared to the control group. Abdominal fat decreased (p<0.05) upon dietary zinc and vitamin A supplementation. Supplemental treatments resulted in an increased total serum protein but decreased glucose, cholesterol, and MDA concentrations. The results of the study show that, separtely or as a combination, zinc and vitamin A supplementation resulted in an improved live weight gain, feed efficiency, and carcass traits, as well as a decrease in serum MDA concentrations. The results of the present study also suggest that zinc and vitamin A have similar effects and that a combination of zinc and vitamin A may offer a potential protective management practice in preventing heat-stress-related depression in performance of broiler chickens.

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References

  1. A. Donkoh, Ambient temperature: a factor affecting performance and physiological response of broiler chickens, Int. J. Biometeorol. 33, 259–265 (1989).

    Article  PubMed  CAS  Google Scholar 

  2. H. S. Siegel, Stress, strains and resistance, Br. Poult. Sci. 36, 3–20 (1995).

    PubMed  CAS  Google Scholar 

  3. S. E. Evans and D. L. Ingram, The effect of ambient temperature upon the secretion of thyroxine in the young pig, J. Physiol. 264, 511–520 (1977).

    PubMed  CAS  Google Scholar 

  4. S. J. Bowen and K. W. Washburn, Thyroid and adrenal response to heat stress in chickens and quail differing in heat tolerance, Poult. Sci. 64, 149–154 (1985).

    PubMed  CAS  Google Scholar 

  5. F.M.A. McNabb and D. B. King, Thyroid hormones effect on growth development and metabolism, in The Endocrinology of Growth Development and Metabolism in Vertebrates, A. Schreibman, ed., Zoological Science, Academic Press, New York Vol. 10, pp. 873–885 (1993).

    Google Scholar 

  6. F. W. Edens and H. S. Siegel, Adrenal respnses in high and low ACTH response lines of chickens during acute heat stress, Gen. Comp. Endocrinol. 25, 64–73 (1975).

    Article  PubMed  CAS  Google Scholar 

  7. B. A. Freeman and J. D. Crapo, Biology of disease: free radicals and tissue injury, Lab. Invest. 47, 412–426 (1982).

    PubMed  CAS  Google Scholar 

  8. K. Sahin, N. Sahin, M. Onderci, S. Yaralioglu, and O. Kucuk, Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress, Vet. Med. Czech. 46, 140–144 (2001).

    CAS  Google Scholar 

  9. M. O. Smith and R. G. Teeter, Potassium balance of the 5 to 8-week old broiler exposed to constant heat or cycling high temperature stress and the effects of supplemental potassium chloride on body weight gain and feed efficiency, Poult. Sci. 66, 487–492 (1987).

    PubMed  CAS  Google Scholar 

  10. O. El Husseiny and C. R. Creger, Effect of ambient temperature on mineral retention and balance of the broiler chicks, Poult. Sci. 60(Suppl. 1), 1651 (1981) (abstract).

    Google Scholar 

  11. N. Sahin, K. Sahin, and O. Kucuk, Effects of vitamin E and vitamin A supplementation on performance, thyroid status, and serum concentrations of some metabolites and minerals in broilers reared under heat stress (32C), Vet. Med. Czech. 46, 286–292 (2001).

    CAS  Google Scholar 

  12. N. Sahin, M. Onderci, and K. Sahin, Effects of dietary chromium and zinc on egg production, egg quality and some blood metabolites of laying hens reared under low ambient temperature, Biol. Trace Element Res. 85, 47–58 (2002b).

    Article  CAS  Google Scholar 

  13. K. Sahin, N. Sahin, M. Sari, and M. F. Gursu, Effects of vitamins E and A supplementation on lipid peroxidation and concentration of some mineral in broilers reared under heat stress (32°C), Nutr. Res. 22, 723–731 (2002e).

    Article  CAS  Google Scholar 

  14. H. Lin, H. Wang, L. F. Song, J. L. Xie, and Y. M. Yang, Effect of dietary supplemental levels of vitamin A on the egg production and immune responses of heat-stressed laying hens, Poult. Sci. 81, 458–465 (2002).

    PubMed  CAS  Google Scholar 

  15. K. Sahin, O. Kucuk, N. Sahin, and M. Sari, Effects of vitamin C and vitamin E on lipid peroxidation status, some serum hormone, metabolite, and mineral concentrations of Japanese quails reared under heat stress (34°C), Int. J. Vitam. Nutr. Res. 72, 91–100 (2002c).

    Article  PubMed  CAS  Google Scholar 

  16. L. R. McDowell, Vitamins in animal nutrition, compatative aspects to human nutrition, Vitamin E, Academic, London, pp. 93–131 (1989).

    Google Scholar 

  17. L. D. McBean, Zinc in Human Nutrition, National Livestock and Meat Board, Chicago, IL (1991).

    Google Scholar 

  18. W. C. Pond, D. C. Church, and K. R. Pond, Zinc in Basic Animal Nutrition and Feeding, 4th ed., Wiley, New York, pp. 190–193 (1995).

    Google Scholar 

  19. M. C. Linder, Nutrition and metabolism of the trace elements, in Nutritional Biochemistry and Metabolism with Clinical Applications, M. C. Linder, ed., Elsevier, New York, pp. 215–276 (1991).

    Google Scholar 

  20. J. C. Smith, Jr., E. G. McDaniel, F. F. Fan, and J. A. Halsted, Zinc: a trace element essential in vitamin A metabolism, Science 181, 954–955 (1973).

    Article  PubMed  CAS  Google Scholar 

  21. P. Christian and K. P. West, Jr., Interactions between zinc and vitamin A, Am. J. Clin. Nutr. 68,(2 Suppl.), 435–441 (1998).

    Google Scholar 

  22. N. I. Berzin and V. K. Bauman, Vitamin-A-dependent zinc-binding protein and intestinal absorption of Zn in chicks, Br. J. Nutr. 57, 255–268 (1987).

    Article  PubMed  CAS  Google Scholar 

  23. NRC, Nutrient Requirements of Poultry, 9th rev. ed., National Academy Press, Washington, DC (1994).

    Google Scholar 

  24. AOAC, Official Methods of Analysis, 15th ed., Association of Official Analytical Chemists, Arlington, VA (1990).

    Google Scholar 

  25. Z. A. Placer, L. L. Cushmann, and B. C. Johnson, Estimation of products of lipid peroxidation in biochemical systems, Anal. Biochem. 16, 359–364 (1966).

    Article  PubMed  CAS  Google Scholar 

  26. B. Matkovics, I. Szabo, and I. S. Varga, Determination of enzyme activities in lipid peroxidation and glutathione pathways, Lab. Diagn. 15, 248–249 (1989).

    Google Scholar 

  27. SAS Institute, SAS User’s Guide: Version 6.12, SAS Institute Inc., Cary, NC (1996).

    Google Scholar 

  28. M. E. Ensminger, J. E. Oldfield, and W. W. Heinemann, Feeds and Nutrition, Ensminger, pp. 8–110 (1990).

  29. S. Hurwitz, M. Weiselberg, U. Eisner, et al., The energy requirements and performance of growing chickens and turkeys, as affected by environmental temperature, Poult. Sci. 59, 2290–2299 (1980).

    CAS  Google Scholar 

  30. E. Demir, O. Öztrikcan, M. Görglü H. R. Kutlu, and F. Okan, Sicak kosullarda yumurta tavugu rasyonlarina eklenen vitamin A ve C’nin yumurta oelliklerine etkileri, J. Agric. Fac. Ç.Ü. 10, 123–132 (1995).

    Google Scholar 

  31. K. I. Brown and K. E. Nestor, Some physiological responses of turkeys selected for high and low adrenal response to cold stress, Poult. Sci. 52, 1948–1952 (1973).

    PubMed  CAS  Google Scholar 

  32. K. D. Roberson and H. M. Edwards, Jr., Effects of 1.25 dihydroxycholecalciferol and phytase on zinc utilization in broiler chicks, Poult. Sci. 73, 1312–1316 (1994).

    PubMed  CAS  Google Scholar 

  33. R. E. Moreng, D. Balnave, and D. Zhang, Dietary zinc methionine effect on eggshell quality of hens drinking saline water, Poult. Sci. 71, 1163–1167 (1992).

    PubMed  CAS  Google Scholar 

  34. R. J. Rothbaum, P. R. Maur, and M. K. Farrel, Serum alkaline phosphatase and zinc under nutrition in infants with chronic diarrhea, Am. J. Clin. Nutr. 35, 595–598 (1982).

    PubMed  CAS  Google Scholar 

  35. J. V. Brandae-Neto, B. B. Stefan, W. Mendonca, A. Bloise, and W. B. Castro, The essential role of zinc in growth. Nutr. Res. 15, 335–358 (1995).

    Article  Google Scholar 

  36. S. Kourtou, T. E. Patiroglu, and S. E. Karakas, Effect of growth hormone on epiphysial growth plates in zinc deficiency, Tokai J. Exp. Clin. Med. 12, 325–329 (1995).

    Google Scholar 

  37. M. J. Salgueiro, N. Krebs, M. B. Zubillaga, et al., Zinc and diabetes mellitus: is there a need of zinc supplementation in diabetes mellitus patients? Biol. Trace. Element Res. 81, 215–228 (2001).

    Article  CAS  Google Scholar 

  38. D. C. Luadicina and L. J. Marnett, Enhancement of hydroperoxide-dependent lipid peroxidation in rat liver microsomes by ascorbic acid, Arch. Biochem. Biophys. 278, 73–80 (1990).

    Article  Google Scholar 

  39. B. Halliwell and J.M.C. Gutteridge, Free Radicals in Biology and Medicine, 2nd ed., Oxford University Press, New York (1989).

    Google Scholar 

  40. R. Feenster, High temperatures decrease vitamin utilization, Misset Poult. 38, 38–40 (1985).

    Google Scholar 

  41. K. C. Klasing, Comparative Avian Nutrition, Cambridge University Press, Cambridge, pp. 277–299 (1993).

    Google Scholar 

  42. M. Zago and P. I. Oteiza, The antioxidant properties of zinc: interactions with iron and antioxidants, Free Radical Biol. Med. 31, 266–274 (2001).

    Article  CAS  Google Scholar 

  43. A. S. Prasad, The role of zinc in brain and nerve functions, in Metals and Oxidative Damage in Neurological Disorders, A. Connor, ed., Plenum, New York, pp. 95–111 (1997).

    Google Scholar 

  44. P.N.B. Gibbs, M. G. Gore, and P. M. Jordan, Investigation of the effect of metal ions on the reactivity of thiol groups in human 5-aminolaevulinate dehydratase. Biochem. J. 225, 573–580 (1985).

    PubMed  CAS  Google Scholar 

  45. P. I. Oteiza, K. L. Olin, C. G. Fraga, and C. L. Keen, Zn deficiency causes oxidative damage to proteins, lipids and DNA in rat testes, J. Nutr. 125, 823–829 (1995).

    PubMed  CAS  Google Scholar 

  46. P. I. Oteiza, K. L. Olin, C. G. Fraga, and C. L. Keen, Oxidant defense systems in testes from Zn deficient rats, Proc. Soc. Exp. Biol. Med. 213, 85–91 (1996).

    PubMed  CAS  Google Scholar 

  47. J. P. Burke and M. R. Fenton, Effect of a Zn-deficient diet on lipid peroxidation in liver and tumor cellular membranes, Proc. Soc Exp. Biol. Med. 179, 187–191 (1985).

    PubMed  CAS  Google Scholar 

  48. P. I. Oteiza, M. S. Clegg, M. P. Zago, and C. L. Keen, Zinc deficiency induces oxidative stress and AP-1 activation in 3T3 cells, Free Radical Biol. Med. 28, 1091–1099 (2000).

    Article  CAS  Google Scholar 

  49. D. J. Tate, M. V. Miceli, and D. A. Newsome, Zn protects against oxidative damage in cultured human retinal pigment epithelial cells, Free Radical Biol. Med. 26, 704–713 (1999).

    Article  CAS  Google Scholar 

  50. S. R. Powell, The antioxidant properties of zinc, J. Nutr. 130, 447S-454S (2000).

    Google Scholar 

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Authors and Affiliations

  1. Department of Animal Nutrition, Faculty of Veterinary, University of Firat, 23100, Elazig, Turkey

    Osman Kucuk & Kazim Sahin

  2. Veterinary Control and Research Institute, 23100, Elazig, Turkey

    Nurhan Sahin

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Kucuk, O., Sahin, N. & Sahin, K. Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chickens. Biol Trace Elem Res 94, 225–235 (2003). https://doi.org/10.1385/BTER:94:3:225

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  • DOI: https://doi.org/10.1385/BTER:94:3:225

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