Abstract
The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of managanese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.
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References
E. J. Underwood, Trace Elements in Human and Animal Nutrition, 4th. ed., Academic, New York (1977).
D. Katz, D. Mazor, A. Dvilansky, and N. Meyerstein, Effect of radiation on red cell membrane and intracellular oxidative defense systems, Free Radical Res. 24(3), 199–204 (1996).
L. H. Schwartz, T. Urban, and S. Herzberg, Antioxidant minerals and vitamins. Role in cancer prevention, Presse Med. 23(39), 1826–1830 (1994).
P. L. Leung and X. L. Lsi, Multielement analysis in serum of thyroid cancer patients before and after a surgical operation, Biol. Trace Element Res. 51(3), 259–266 (1996).
I. Fridovich, Superoxide radical and superoxide dismutases, Annu. Rev. Biochem. 64, 97–112 (1995).
L. LCostanzo, G. De-Guidi, S. Giuffrida, S. Sortino, and G. Condorelli, Antioxidant effect of inorganic ions on UVC and UVB induced lipid peroxidation, J. Inorg. Biochem. 59(1), 1–13 (1995).
A. Molteni, W. F. Ward, Y. T. Kim, et al., Serum copper concentration as an index of clinical lung injury, Adv. Exp. Med. Biol., 258, 273–285 (1989).
E. Aladzhov and A. Logofetov, Acute-phase changes in ceruloplasmin after gamma-ray irradiation, Eksp. Med. Morfol. 29(2), 8–12 (1990).
J. Chatterjee, B. B. Mukherjee, K. De, A. K. Das, and S. K. Basu, Trace metal levels of X-ray technicians’ blood and hair, Biol. Trace Element Res. 46(3), 211–227 (1994).
A. Petkau, W. S. Chelack, S. D. Pleskack, B. E. Meeker, and C. M. Brady, Radioprotection of mice by superoxide dismutase, Biochem. Biophys. Res. Commun. 65(3), 886–893 (1975).
W. E. Greenberg, R. R. Trusell, and L. S. Clescer, Standart Methods for the Examination of Water and Wastewater, 60th ed. American Public Health Association, Washington, DC, pp. 143–179 (1996).
J. E. Longbottom, T. D. Martin, K. W. Edgell, S. E. Long, M. R. Plantz, and B. E. Warden, Determination of trace elements in water by inductively coupled plasma-mass spectrometry: collaborative study, J. AOAC Int. 77(4), 1004–1023 (1994).
T. Nakano, K. Oka, and N. Taniguchi, Manganese superoxide dismutase expression correlates with P53 status and local recurrence of cervical carcinoma treated with radiation therapy, Cancer Res. 56(12), 2771–2775 (1996).
M. R. Zhou, J. F. Weng, Z. I. Wang, and K. M. Shao, Study on the effect of copper, zinc, manganese and selenium on growth performance and some blood physiological and biochemical parameters in broiler chicks, Nutr. Abstr. Rev. (Ser. B) 64(10), 4989 (1994).
J. F. Weiss and M. R. Landauer, Radioprotection by antioxidants, Ann. NY Acad. Sci. 899, 44–60 (2000).
C. K. Lii, Y. J. Ko, M. T. Chiang, W. C. Sung, and H. W. Chen, Effect of dietary vitamin E on antioxidant status and antioxidant enzyme activities in Sprague-Dawley rats. Nutr. Cancer 32(2), 95–100 (1998).
K. Umegaki, M. Sano, K. Suzuki, I. Tomita, and T Esashi, Increases in 4-hydroxynoneal and hexanal in bone marrow of rats subjected to total body x ray irradiation: association with antioxidant vitamins, Bone Marrow Transplant., 23, 173–178 (1999).
U. Mutlu-Türkoğlu, Y. Erbil, S. Öztezcan, V. Olgaç, G. Toker, and M. Uysal, The effect of selenium and/ or vitamin E treatments on radiation-induced intestinal injury in rats, Life Sci. 66(20), 1905–1913 (2000).
L. A. Fomenko, T. A. Bezlepkina, A. N. Anoshkin, and A. I. Gaziev, A vitamin-antioxidant diet decreases the level of chromosomal damages and the frequency of gene mutations in irradiated mice, Izv. Akad. Nauk Ser. Biol. 4, 419–424 (1994).
E. Russanov, P. Balevska, and S. Leutshev, X-irradiation effects on lipid peroxidation and superoxide dismutase and catalase activities in copper deficient and copper loaded rat liver, Acta Physiol. Pharmacol. Bulg. 5(2), 73–78 (1979).
H. Ogata and Y. Izumo, Mortality reduction in mice administered a single abundant dose of zinc, manganese or magnesium after irradiation by gamma-rays at sublethal doses. Radioisotopes 39(12), 573–576 (1990).
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Dede, S., Değer, Y., Mert, N. et al. Studies on the effects of X-ray on erythrocyte zinc and copper concentrations in rabbits after treatment with antioxidants. Biol Trace Elem Res 92, 55–60 (2003). https://doi.org/10.1385/BTER:92:1:55
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DOI: https://doi.org/10.1385/BTER:92:1:55