Skip to main content
SpringerLink
Log in

Optimization of Brewery's spent grain dilute-acid hydrolysis for the production of pentose-rich culture media

  • Session 6A Biomass Pretreatment and Hydrolysis
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Dilute-acid hydrolysis of brewery's spent grain to obtain a pentose-rich fermentable hydrolysate was investigated. The influence of operational conditions on polysaccharide hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.39–3.06. When the CS increased, the pentose sugars concentration increased to a maximum at a CS of 1.94, whereas the maximum glucose concentration was obtained for a CS of 2.65. The concentrations of furfural, hydroxymethylfurfural (HMF), as well as formic and levulinic acids and total phenolic compounds increased with severity. Optimum hydrolysis conditions were found at a CS of 1.94 with >95% of feedstock pentose sugars recovered in the monomeric form, together with a low content of furfural, HMF, acetic and formic acids, and total phenolic compounds. This hydrolysate containing glucose, xylose, and arabinose (ratio 10∶67∶32) was further supplemented with inorganic salts and vitamins and readily fermented by the yeast Debaryomyces hansenii CCMI 941 without any previous detoxification stage. The yeast was able to consume all sugars furfural, HMF, and acetic acid with high biomass yield, 0.68C-mol/C-mol, and productivity, 0.92 g/(L·h). Detoxification with activated charcoal resulted in a similar biomass yield and a slight increase in the volumetric productivity (11%).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gírio, F. M., Carvalheiro, F., Esteves, M. P., Amaral-Collaço, M. T., Parajó, J. C., Domínguez, H., et al. (2003) Portuguese patent no. 102563.

  2. Kabel, M. A., Schols, H. A., and Voragen, A. G. J. (2002), Carbohydr. Polym. 50, 191–200.

    Article  CAS  Google Scholar 

  3. Roberto, I. C., Felipe, M. G. A., Lacis, L. S., Silva, S. S., and Mancilha, I. M. (1991), Bioresour. Technol. 36, 271–275.

    Article  CAS  Google Scholar 

  4. Roberto, I. C., Felipe, M. G. A., Mancilha, I. M., Vitolo, M., Sato, S., and Silva, S. S. (1995), Bioresour. Technol. 51, 255–257.

    Article  CAS  Google Scholar 

  5. McMillan, J. D. (1994), in Enzymatic Conversion of Biomass for Fuels Production Himmel, M. E., Baker, J. O., and Overend, R. P., eds., ACS Symposium Series 566, American Chemical Society, Washington, DC, pp. 411–437.

    Google Scholar 

  6. Saha, B. C. and Bothast, R. J. (1996), Appl. Microbiol. Biotechnol. 45, 299–306.

    Article  CAS  Google Scholar 

  7. Palmqvist, E. and Hahn-Hägerdal, B. (2000), Bioresour. Technol. 74, 17–24.

    Article  CAS  Google Scholar 

  8. van Walsum, G. P., Allen, S. G., Spencer, M. J., Laser, M. S., Antal, M. J., and Lynd, L. R. (1996), Appl. Biochem. Biotechnol. 57/58, 157–170.

    Google Scholar 

  9. Allen, S. G., Schulman, D., Lichwa, J., Antal, M. J., Jennings, E., and Elander, R. (2001), Ind. Eng. Chem. Res. 40, 2352–2361.

    Article  CAS  Google Scholar 

  10. Allen, S. G., Schulman, D., Lichwa, J., Antal, M. J., Laser, M., and Lynd, L. R. (2001), Ind. Eng. Chem. Res. 40, 2934–2941.

    Article  CAS  Google Scholar 

  11. Torget, R., Hatzis, C., Hayward, T. K., Hsu, T. A., and Philippidis, G. P. (1996), Appl. Biochem. Biotechnol. 57/58, 85–101.

    Article  CAS  Google Scholar 

  12. Allen, S. G., Kam, L. C., Zemann, A. J., and Antal, M. J. (1996), Ind. Eng. Chem. Res. 35, 2709–2715.

    Article  CAS  Google Scholar 

  13. Mok, W. S. L. and Antal, M. J. (1992), Ind. Eng. Chem. Res. 31, 1157–1161.

    Article  CAS  Google Scholar 

  14. Saska, M. and Ozer, E. (1995), Biotechnol. Bioeng., 45, 517–523.

    Article  CAS  Google Scholar 

  15. Heitz, M., Capek-Ménard, E., Koeberle, P. G., Gagne, J., Chornet, E., Overend, R. P., Taylor, J. D., and Yu, E. (1991), Bioresour. Technol. 35, 23–32.

    Article  CAS  Google Scholar 

  16. Garrote, G., Domínguez, H., and Parajó, J. C. (2001), Appl. Biochem. Biotechnol. 95, 195–207.

    Article  PubMed  CAS  Google Scholar 

  17. Duarte, L. C., Carvalheiro, F., Lopes, S., Marques, S., Parajó, J. C., and Gírio F. M. (2004), Appl. Biochem. Biotechnol. 113–116, 1139–1056.

    Google Scholar 

  18. Vázquez, M. J., Alonso, J. L., Domínguez, H., and Parajó, J. C. (2001), World J. Microbiol. Biotechnol. 17, 817–822.

    Article  Google Scholar 

  19. Domínguez, J. M., Cao, N. J., Gong, C. S. and Tsao, G. T. (1997), Bioresour Technol. 61, 85–90.

    Article  Google Scholar 

  20. Chum, H. L., Johnson, D. K., Black, S. K., and Overend, R. P. (1990), Appl. Biochem. Biotechnol. 24/25, 1–14.

    Google Scholar 

  21. Overend, R. P. and Chornet, E. (1987), Phil. Trans. R. Soc. Lond. A321, 523–536.

    ADS  Google Scholar 

  22. Nguyen, Q. A., Tucker, M. P., Keller, F. A., and Eddy, F. P. (2000), Appl. Biochem. Biotechnol. 84–86, 561–576.

    Article  PubMed  Google Scholar 

  23. Tengborg, C., Stenberg, K., Galbe, M., Zacchi, G., Larsson, S., Palmqvist, E., and Hahn-Hägerdal, B. (1998), Appl. Biochem. Biotechnol. 70–72, 3–15.

    Google Scholar 

  24. Nobre, A., Duarte, L. C., Roseiro, J. C., and Gírio, F. M. (2002), Appl. Microbiol. Biotechnol. 59, 509–516.

    Article  PubMed  CAS  Google Scholar 

  25. Browning, B. L. (1967), in Methods in Wood Chemistry, Sarkanen, K. V., and Ludwig, C. H., eds. John Wiley & Sons, New York, NY.

    Google Scholar 

  26. AOAC. (1975), AOAC Official Methods of Analysis, AOAC International, Washington, DC.

    Google Scholar 

  27. Graham, H. D. (1992), J. Agric. Food Chem. 40, 801–805.

    Article  CAS  Google Scholar 

  28. Carrasco, J. E., Sáiz, M. C., Navarro, A., Soriano, P., Sáez, F., and Martínez, J. M. (1994), Appl. Biochem. Biotechnol. 45/46, 23–34.

    Google Scholar 

  29. Torget, R., Werdene, P., Himmel, M., and Grohmann, K. (1990), Appl. Biochem. Biotechnol. 24/25, 115–126.

    Google Scholar 

  30. Torget, R., Walter, P., Himmel, M., and Grohmann, K. (1991), Appl. Biochem. Biotechnol. 28/29, 75–86.

    Article  Google Scholar 

  31. Grohmann, K., Torget, R., and Himmel, M. (1985), Biotechnol. Bioeng. Symp. 15, 59–80.

    Google Scholar 

  32. Taherzadeh, M. J., Eklund, R., Gustafsson, L., Niklasson, C., and Liden, G. (1997), Ind. Eng. Chem. Res. 36, 4659–4665.

    Article  CAS  Google Scholar 

  33. Martin, C., Galbe, M., Nilverbrant, N. O., and Jönsson, L. J. (2002), Appl. Biochem. Biotechnol. 98–100, 699–716.

    Article  PubMed  Google Scholar 

  34. Neureiter, M., Danner, H., Thomasser, C., Saidi, B., and Braun, R. (2002), Appl. Biochem. Biotechnol. 98–100, 49–58.

    Article  PubMed  Google Scholar 

  35. Aoyama, M., Seki, K., and Saito, N. (1995), Holzforschung 49, 193–196.

    Article  CAS  Google Scholar 

  36. Montané, D., Salvadó, J., Farriol, X., Jollez, P., and Chornet, E. (1994), Wood Sci. Technol. 28, 387–402.

    Article  Google Scholar 

  37. Palmqvist, E., Grage, H., Meinander, N. Q., and Hahn-Hägerdal, B. (1999), Biotechnol. Bioeng. 63, 46–55.

    Article  PubMed  CAS  Google Scholar 

  38. Domínguez, J. M., Gong, C. S., and Tsao, G. T. (1996), Appl. Biochem. Biotechnol. 57/58, 49–56.

    Google Scholar 

  39. Parajó, J. C., Domínguez H., and Domínguez, J. M. (1996), Bioresour Technol. 57, 179–185.

    Article  Google Scholar 

  40. Miyafuji, H., Danner, H., Neureiter, M., Thomasser, C., Bvochora, J., Szolar, O., and Braun, R. (2003), Enzyme Microb. Technol. 32, 396–400.

    Article  CAS  Google Scholar 

  41. Larsson, S., Reimann, A., Nilverbrant, N. O., and Jönsson, L. J. (1999), Appl. Biochem. Biotechnol. 77–79, 91–103.

    Article  Google Scholar 

  42. Nilvebrant, N. O., Reimann, A., Larsson, S., and Jönsson L. J. (2001), Appl. Biochem. Biotechnol. 91–93, 35–49.

    Article  PubMed  Google Scholar 

  43. Carvalheiro, F., Duarte, L. C., Lopes, S., Parajó, J. C., Pereira, H., and Gírio, F. M. (2003), submitted.

  44. Roberto, I. C., Mancilha, I. M., Souza, C. A., Felipe, M. G. A., Sato, S., and Castro, H. F. (1994), Biotechnol. Lett. 16, 1211–1216.

    Article  CAS  Google Scholar 

  45. Pessoa, A., Mancilha, I. M., and Sato, S. (1996), J. Biotechnol. 51, 83–88.

    Article  CAS  Google Scholar 

  46. Roseiro, J. C., Peito, M. A., Gírio F. M., and Amaral-Collaço, M. T. (1991), Arch. Microbiol. 156, 484–490.

    CAS  Google Scholar 

  47. Tavares, J. M., Duarte, L. C., Amaral-Collaço, M. T., and Gírio, F. M. (2000), Enzyme Microbiol. Technol. 26, 743–747.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departmento de Biotecnologia, INETI, Estrada do Paço do Lumiar 22, 1649-038, Lisboa, Portugal

    Florbela Carvalheiro, Luís C. Duarte, Raquel Medeiros & Francisco M. Gírio

Authors
  1. Florbela Carvalheiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luís C. Duarte
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raquel Medeiros
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Francisco M. Gírio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Francisco M. Gírio.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carvalheiro, F., Duarte, L.C., Medeiros, R. et al. Optimization of Brewery's spent grain dilute-acid hydrolysis for the production of pentose-rich culture media. Appl Biochem Biotechnol 115, 1059–1072 (2004). https://doi.org/10.1385/ABAB:115:1-3:1059

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:115:1-3:1059

Index Entries

Search

Navigation