Summary
Legumes have long been recalcitrant to efficient Agrobacterium tumefaciens-mediated transformation. The choice and use of model legume plants (Medicago truncatula and Lotus japonicus) for molecular studies has triggered extensive studies devoted to the development of efficient Agrobacterium-mediated transformation protocols for these two plants. In M. truncatula, transformation protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on Agrobacterium-mediated transformation of M. truncatula followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for M. truncatula R108-1 (c3).
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This work is dedicated to Dr. Hanh Trinh who initiated this protocol.
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Cosson, V., Durand, P., d'Erfurth, I., Kondorosi, A., Ratet, P. (2006). Medicago truncatula Transformation Using Leaf Explants. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 343. Humana Press. https://doi.org/10.1385/1-59745-130-4:115
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DOI: https://doi.org/10.1385/1-59745-130-4:115
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