Abstract
Over the past 15 yr, the use of transgenic mice has led to significant advances in our understanding of immunological tolerance. In a normal repertoire the number of B cells with a single antigen receptor specificity is very small, making the study of their fate difficult. In contrast, animals that carry transgenes encoding rearranged immunoglobulin genes generate large numbers of B cells that, by the process of allelic exclusion, have an identical specificity. Exploitation of this effect has enabled the mechanisms involved in B-cell tolerance to be explored in some detail.
In this review we use the hen egg lysozyme (HEL) model system to illustrate the generation and preparation of a transgene. In our example, we describe the generation of mice expressing HEL as a systemic, intracellular, membrane-bound self-antigen. The same principles and methods apply to immunoglobulin transgenes. We briefly discuss the techniques that could be used to explore mechanisms of tolerance to systemic intracellular antigens in these mice.
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Ferry, H., Cornall, R.J. (2004). Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice. In: Gu, H., Rajewsky, K. (eds) B Cell Protocols. Methods in Molecular Biology, vol 271. Humana Press. https://doi.org/10.1385/1-59259-796-3:239
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DOI: https://doi.org/10.1385/1-59259-796-3:239
Publisher Name: Humana Press
Print ISBN: 978-1-58829-347-3
Online ISBN: 978-1-59259-796-3
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