Abstract
In cells, the poly(A) tail stimulates translation from messenger RNAs bearing a cap structure or viral IRES elements. This 3′ end-mediated stimulation of translation is not reflected in commonly used commercial cell-free translation systems prepared from rabbit reticulocytes or wheat germ. We describe a simple procedure to generate poly(A) tail-responsive translation extracts from HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines.
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© 2004 Humana Press Inc., Totowa, NJ
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Thoma, C., Ostareck-Lederer, A., Hentze, M.W. (2004). A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells. In: Schoenberg, D.R. (eds) mRNA Processing and Metabolism. Methods in Molecular Biology™, vol 257. Humana Press. https://doi.org/10.1385/1-59259-750-5:171
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DOI: https://doi.org/10.1385/1-59259-750-5:171
Publisher Name: Humana Press
Print ISBN: 978-1-58829-225-4
Online ISBN: 978-1-59259-750-5
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