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A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization

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Nitric Oxide Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 100))

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Abstract

In-situ hybridization (ISH) is a technique that allows the demonstration of nucleic-acid sequences of interest within tissues and their localization to subpopulations of cells within those tissues.

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References

  1. Cook, H. T., Bune, A. J., Jansen, A. S., Taylor, G. M., Loi, R. K., and Cattell, V. (1994) Cellular localization of inducible nitric oxide synthase in experimental endotoxic shock in the rat. Clin.Sci. 87, 179–186.

    PubMed  CAS  Google Scholar 

  2. Davis, L. G., Dibner, M. D., and Battey, J. F., eds. (1986) Hybridisation with synthetic end-labelled probes, in, Basic Methods in Molecular Biology, Elsevier, New York, pp 75–78.

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  3. Lyons, C. R., Orloff, G. J., and Cunningham, J. M. (1993) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J. Biol. Chem. 267, 6370–6374.

    Google Scholar 

  4. Nunokawa, Y., Ishida, N., and Tanaka, S. (1993) Cloning of inducible nitric oxide synthase in rat vascular smooth muscle cells. Biochem. Biophys. Res. Commun. 191, 89–94.

    Article  PubMed  CAS  Google Scholar 

  5. Geller, D. A., Lowenstein, C. J., Shapiro, R. A., Nussler, A. K., Disilvio, M., Wang, S. C., Nakayama, D. K., Simmons, R. L., Snyder, S. H., and Billiar, T. R. (1993) Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. Proc. Natl. Acad. Sci. USA 90, 3491–3495.

    Article  PubMed  CAS  Google Scholar 

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© 1998 Humana Press Inc.

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Taylor, G.M., Cook, H.T. (1998). A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization. In: Titheradge, M.A. (eds) Nitric Oxide Protocols. Methods in Molecular Biology™, vol 100. Humana Press. https://doi.org/10.1385/1-59259-749-1:191

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  • DOI: https://doi.org/10.1385/1-59259-749-1:191

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-470-9

  • Online ISBN: 978-1-59259-749-9

  • eBook Packages: Springer Protocols

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