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Affinity Measurement Using Surface Plasmon Resonance

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Antibody Engineering

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 248))

Abstract

The change in free energy for a reaction, ΔG, can be determined from the dissociation affinity constant, K D , since ΔG is equal to RTlnK D . The affinity constant provides no information on the rate of the reaction or the energy required to reach the transition state of the reaction (Fig. 1).

Energy vs reaction coordinates. A=analyte, L=ligand.

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Authors and Affiliations

  1. Biacore AB, Uppsala, Sweden

    Robert Karlsson & Anita Larsson

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  1. Robert Karlsson
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  2. Anita Larsson
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  1. MRC Laboratory of Molecular Biology, University of Cambridge, Cambridge, UK

    Benny K. C. Lo

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© 2004 Humana Press Inc., Totowa, NJ

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Karlsson, R., Larsson, A. (2004). Affinity Measurement Using Surface Plasmon Resonance. In: Lo, B.K.C. (eds) Antibody Engineering. Methods in Molecular Biology™, vol 248. Humana Press. https://doi.org/10.1385/1-59259-666-5:389

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  • DOI: https://doi.org/10.1385/1-59259-666-5:389

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-092-2

  • Online ISBN: 978-1-59259-666-9

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