Abstract
High-density polynucleotide probe arrays provide a massively parallel approach to genetic sequence analysis that is having a major impact on biomedical research and clinical diagnostics (1). These arrays are comprised of large sets of nucleic acid probe sequences immobilized in defined, addressable locations on the surface of a substrate, and are capable of acquiring unprecedented amounts of genetic information from biological samples in a single hybridization procedure. The advent of this technology has relied on developing methods of fabricating arrays with sufficiently high information content and density. Light-directed synthesis (2–5) has enabled the large-scale manufacture of arrays containing hundreds of thousands of oligonucleotide probe sequences on a glass “chip” about 1.6 cm2. This method is used to produce high-density GeneChip® probe arrays, which are now finding widespread use in the detection and analysis of mutations and polymorphisms (“genotyping”), and in a wide range of gene expression studies.
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McGall, G.H., Fidanza, J.A. (2001). Photolithographic Synthesis of High-Density Oligonucleotide Arrays. In: Rampal, J.B. (eds) DNA Arrays. Methods in Molecular Biology™, vol 170. Humana Press. https://doi.org/10.1385/1-59259-234-1:71
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DOI: https://doi.org/10.1385/1-59259-234-1:71
Publisher Name: Humana Press
Print ISBN: 978-0-89603-822-6
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