Abstract
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is fast becoming the marker of choice for protein localization and gene expression studies. The rapid rise in popularity stems from several advantages over traditional markers such as β-galactosidase and alkaline phosphatase, which typically require the addition of exogenous substrates for detection. The unique intrinsic spectral properties of GFP allow it to be directly visualized in fixed or live cells by fluorescence microscopy without the addition of cofactors or exogenous substrates (1–8).
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Zirngibl, R.A., Greer, P. (2000). Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins. In: Reith, A.D. (eds) Protein Kinase Protocols. Methods in Molecular Biology™, vol 124. Humana Press. https://doi.org/10.1385/1-59259-059-4:195
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DOI: https://doi.org/10.1385/1-59259-059-4:195
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