Abstract
The chemokine superfamily is a large group of small proteins, which share a limited amount of primary sequence identity, although a highly conserved three-dimensional subunit structure. The original members of the superfamily, Interleukin-8 and MCP-1 were purified over 10 years ago. Following on from these discoveries, many more of these proteins were identified because of their ability to selectively recruit and activate specific leukocyte populations. From these initial discoveries, the number of chemokines that have been discovered has increased almost exponentially in the last two years. Two main factors have been key to the discovery of so many new chemokines: first, the availability of large amounts of sequence information gained by random sequencing of cDNA libraries generating expressed sequence tag collections (ESTs); and second, the availability of this information on-line to research groups via the world wide web. This has meant the rapid increase in the number of chemokines that are currently well characterized. An additional spin-off of these novel chemokines is that a number of putative chemokine receptors have been paired up with their ligands over the last year.
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References
Tashiro K., Tada H., Heiker R., Shirozu M., Nak-ano T., and Honjo T. (1993) Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science,261, 600–603.
Imai T., Baba M., Nishimura M., Kakizaki M., Takagi S., and Yoshie O. (1997) The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J. Biol. Chem. 272, 15,036-15,042.
Wells T. N. C. and Peitsch M. C. (1997) The chemokine information source: identification and chracterization of novel chemokines using the WorldWide Web and expressed sequence tag databases. J. Leuk. Biol. 61, 545–550.
Yoshie O., Imai T., and Nomiyama H. (1997) Novel lymphocyte-specific CC chemokines and their receptors. J. Leuk. Biol. 62,634–644.
Proudfoot A. E., Power C. A., Hoogewerf A. J., Montjovent M. O., Borlat F., Offord R. E., and Wells T. N. C. (1996) Extension of recombinant human RANTES by the retention of the initiating methionine produces a potent antagonist. J. Biol. Chem. 271, 2599–2603.
Coulin F., Power C. A., Alouani S., Peitsch M. C., Schroeder J.-M., Moshizuki M., Clark-Lewis I., and Wells T. N. C. (1997) Characterisation of macrophage inflammatory protein-5/human CC cytokine-2, a member of the macrophage-inflammatory-protein family of chemokines. Eur. J. Biochem. 248, 507–515.
Kledal T. N., Rosenkilde M. M., Coulin F., Simmons G., Johnsen A. H., Alouani S., Power C. A., Luttichau H. R., Gerstoft J., Clapham P. R., Clark-Lewis I., Wells T. N. C., and Schwartz T. W. (1997) A broad-spectrum chemokine antagonist encoded by Kaposi’s sarcoma-associated herpesvirus. Science 277,1656–1659.
Power C. A., Church D. J., Meyer A., Alouani S., Proudfoot A. E. I., Clark-Lewis I., Sozzani S., Mantovani A., and Wells T. N. C. (1997) Cloning and characterization of a specific receptor for the novel CC chemokine MIP-3α from lung dendritic cells. J. Exp. Med. 186, 825–835.
Greaves D. R., Wang W., Dairaghi D. J., Dieu M. C., de Saint-Vis B., Franz K., Rossi D., Caux C., McClanahan T., Gordon S., Zlotnik A., and Schall T. J. (1997) CCR6, a CC chemokine receptor that interacts with macrophage inflammatory protein 3α and is highly expressed in human dendritic cells. J. Exp. Med. 186,837–844.
Yoshida R., Imai T, Hieshima K., Kusssuda J., Baba M., Kitura M., Nishimura M., Kakizaki M., Nomiyama H., and Yoshie O. (1997) Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7. J. Biol. Chem. 272,13,803-13,809.
Gao J.-L. and Murphy P. M. (1994) Human cytomegalovirus open reading frame US28 encodes a functional β chemokine receptor. J. Biol. Chem. 269, 28,539-28,542.
Wells T. N. C. and Schwartz T. W. (1997) Plagiarism of the host immune system: lessons about chemokine immunology from viruses. Curr. Opinion Biotech. 8, 741–748.
Martin W. J., Zeng L. C., Ahmed K., and Roy M. (1994) Cytomegalovirusrelated sequence in an atypical cytopathic virus repeatedly isolated from a patient with chronic fatigue syndrome. Am. J. Pathol. 145,440–451.
Cha T.-A., Tom E., Kemble G. W., Duke G. M., Mocarski E. S., and Spaete R. R. (1996) Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J. Virol. 70, 78–83.
Wells T. N. C., Graber P., Proudfoot A. E. I., Arod C. Y., Jordan S. R., Lambert M. H., Hassel A. M., and Milburn M. V. (1994) The three-dimensional structure of human interleukin-5 at 2.4 angstroms resolution: implication for the structures of other cytokines. Ann. NYAS 725, 118–127.
Peitsch M. C. (1995) Protein modeling by E-mail. From amino acid sequence to protein structure: a free one-hour service. Bio/Technol. 13, 658–660.
Peitsch M. C. (1996) ProMod and Swiss-model: internet-based tools for automated comparative protein modelling. Biochem. Soc. Trans. 24, 274–279.
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Wells, T.N.C., Peitsch, M.C. (2000). Identification of Novel Chemokines From Expressed Sequence Tag Databases. In: Proudfoot, A.E.I., Wells, T.N.C., Power, C.A. (eds) Chemokine Protocols. Methods in Molecular Biology, vol 138. Humana Press. https://doi.org/10.1385/1-59259-058-6:65
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DOI: https://doi.org/10.1385/1-59259-058-6:65
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