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DNA Sequencing by Capillary Array Electrophoresis

  • Protocol
Capillary Electrophoresis of Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 162))

Abstract

On May 9,1998, PE Biosystems announced that they would introduce a new instrument based on “breakthrough DNA analysis technology” (1). Over 700 orders for the new sequencer were received in the first 5 mo of production, which make this instrument one of the most successful new products in the history of analytical instrumentation. This new instrument is based on capillary array electrophoresis (CAE) with a sheath-flow cuvet detector. This detection technology provides nearly 100% duty cycle, where every capillary is monitored simultaneously without the need for a scanning detector system. By use of this instrument, the Drosophila melanogaster sequence will be completed in less than 5 mo (2), and the sequence of the human genome will be substantially complete by the time that this book is in print.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Washington, Seattle, WA

    Norman J. Dovichi

  2. Molecular Dynamics, Sunnyvale, CA

    JianZhong Zhang

Authors
  1. Norman J. Dovichi
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  2. JianZhong Zhang
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Editor information

Editors and Affiliations

  1. Australian Genome Research Facility, University of Queensland, Brisbane

    Keith R. Mitchelson

  2. Walter & Eliza Hall Institute, Parkville, Australia

    Keith R. Mitchelson

  3. Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

    Jing Cheng

  4. Aviva Biosciences Corporation, San Diego, CA

    Jing Cheng

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© 2001 Humana Press Inc.

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Cite this protocol

Dovichi, N.J., Zhang, J. (2001). DNA Sequencing by Capillary Array Electrophoresis. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology, vol 162. Humana Press. https://doi.org/10.1385/1-59259-055-1:85

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  • DOI: https://doi.org/10.1385/1-59259-055-1:85

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-779-3

  • Online ISBN: 978-1-59259-055-1

  • eBook Packages: Springer Protocols

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