Skip to main content
SpringerLink
Log in

Mycobacteriophages

  • Protocol
Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 101))

Abstract

Mycobacteriophages are viruses that infect the mycobacteria Historically, they have been used to overcome major road blocks in the genetic manipulation of mycobacteria. Mycobacteriophages have been used to demonstrate mycobacterlal transfection (1) and transduction (2). Additionally, difficulties in transformation were solved using shuttle phasmids (3,4), which are chimeras of mycobacteriophage and plasmid DNA that can replicate as plasmids in Escherichia coli and phages in mycobacteria.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Tokunaga, T. and Sellers, M I (1964) Infection of Mycobacterzum smegmatis and D29 phage DNA J Exp Med 119, 139–149.

    Article  PubMed  CAS  Google Scholar 

  2. Sundara J. C. V. and Ramakrishnan, T (1971) Transduction in Mycobacterium smegmatis Nature (London) 228, 280–281

    Google Scholar 

  3. Jacobs, W RJr,., Tuckman, M., and Bloom, B R (1987) Introduction of foreign DNA into mycobacteria using a shuttle phasmid Nature (London) 327, 532–536

    Article  CAS  Google Scholar 

  4. Snapper, S., Lugost, L., Jekkel, A., Melton, R E., Kelser, T, Bloom, B, and Jacobs, W RJr, (1988) Lysogeny and transformation in mycobacteria stable expression of foreign genes. Proc Natl Acad. Sci USA 85, 6987–6991.

    Article  PubMed  CAS  Google Scholar 

  5. Redmond, W. B (1963) Bactertophages of mycobacteria a review Adv Tuberc Rev 12, 191–229.

    Google Scholar 

  6. Barksdale, L. and Kim, K.-S (1977) Mycobact Bacteriol Rev 41, 217–372

    CAS  Google Scholar 

  7. Mtzugucht, Y. (1984) Mycobacteriophages, in The Mycobacteria a Sourcebook, part A (G. P. Kubica and L. G. Wayne eds), Marcel Dekker, Inc, New York, pp 641–662

    Google Scholar 

  8. Engel, H W B. (1978) Mycobacteriophage and phage typing Ann Mzcrobiol (Inst Pasteur) 129A, 75–90

    Google Scholar 

  9. Stover, C. K., de la Cruz, V. F., Feurst, T R, Burlem, J. E., Benson, L. A., Bennet, L T, Bansal, G P, Young, J F., Lee, M. H, Hatfull, G F., Snapper, S B., Barletta, R G, Jacobs, Jr, W R, and Bloom, B R. (1991) New use of BCG for recombinant vaccines Nature (London) 351, 456–460

    Article  CAS  Google Scholar 

  10. Haeseleer, F, Pollet, J. F, Haumont, M., Bollen, A, and Jacobs, P. (1993) Stable integration and expression of the Plasmodzum falczparum cncumsporozotte protein coding sequence in mycobacteria Mol Bzochem Parasitol 57, 117–126

    Article  CAS  Google Scholar 

  11. Donnelly-Wu, M. K., Jacobs, W. R. Jr, and Hatfull, G. F (1993) Supermfectton immumty of mycobactertophage L5: applications for genetic transformation of mycobacteria. Mol Microbiol 7, 407–417.

    Article  PubMed  CAS  Google Scholar 

  12. Barsom, E K. and Hatfull, G F (1996) Characterization of a Mycobacterzum smegmatzs gene that confers resistance to phages L5 and D29 when overexpressed Mol Microbiol 21, 159–170

    Article  PubMed  CAS  Google Scholar 

  13. Furuchl, A. and Tokunaga, T (1972) Nature of the receptor substance of Mycobacterzum smegmatzs for D4 bacteriophage adsorption J Bacteriol. 111, 404–411

    Google Scholar 

  14. Dhariwal, K. R, Liav, A, Vatter, A. E, Dhariwal, G., and Goren, M B (1986) Haptemc ohgosacchartdes in antlgemc variants of mycobacterial C-mycosldes antagonize lipid receptor activity for mycobacteriophage D4 by masking a methylated rhamnose J Bacterzol 168, 283–293

    CAS  Google Scholar 

  15. Basso, G., Castelnuovo, G, Nardelh, M-G, Orefici, G, Arancia, G, Laneelle, G., Asselineau, C, and Asselmeau, J (1976) A study of the receptor for a myco-bacteriophage: phage phlei Blochlmle 58, 87–97

    Google Scholar 

  16. Rado, T A, Bates, J H, and Fnzhugh, J K (1976) Evidence for host-dependent modificatton and restriction of bacteriophage DNA in Mycobacterlum tuberculosis J. Gen Virol 30, 91–97.

    Article  PubMed  CAS  Google Scholar 

  17. Jones, W. D. Jr. and Greensburg, J. (1977) Host modtfication and restriction with a mycobactertophage isolated from a psuedolysogemc Mycobacterzum chelonel J Gen Microbiol 99, 389–395

    PubMed  Google Scholar 

  18. Jones, W DJr, (1980) Typing Mycobacterzum tuberculosis with mycobacterio-phageBo4. J Gen Vzrol 49, 319–322.

    Article  CAS  Google Scholar 

  19. Hatfull, G F and Jacobs, W R. Jr, (1994) Mycobacteriophages cornerstones of mycobacterial research, in Tuberculoszs Pathogeneszs, Protection, and Control (Bloom, B R., ed.), Amencan Society for Microbiology, Washington, D C,pp 165–183

    Google Scholar 

  20. Hatfull, G. F and Sarkis, G J (1993) DNA sequence, structure, and gene expression of mycobactertophage L5* a phage system for mycobacterial genetics Mol Microblol. 7, 395–405.

    Article  CAS  Google Scholar 

  21. Popa, M. P., McKelvey T. A., Hempel, J., and Hendrix, R. W. (1991) Bacteriophage HK97 structure. wholesale covalent cross-lmkmg between the major head shell subunits. J Vwol 65, 3227–3237.

    CAS  Google Scholar 

  22. Crawford, J and Bates, J. H (1984) Phage typing of mycobacteria, in The Mycobacteria a Sourcebook, part A (Kubica, G P and Wayne, L G, eds.), Marcel Dekker, New York, pp 123–132

    Google Scholar 

  23. Grange, J. M. (1982) The genetics of mycobacteria and mycobacteriophages, in The Biology Of Mycobacterza, vol. 1 (Ratledge, C and Stanford, J, eds ), Academic Press, London, pp 309–351.

    Google Scholar 

  24. Froman, S. and Scammon, L (1964) Effect of temperature on the bacteriophage susceptibility of strains of M avutm isolated from fowl. Am Rev Resp Dls 89, 236–239

    CAS  Google Scholar 

  25. Grange, J. M. and Nordstrom, G. (1973) Bacteriophage typing of M. ranae Cforturtum). The correlation of lysis by mycobacteriophage BK4 and mositol utilization. Acta Path Mlcroblol Stand, Sect B 81, 408–412

    CAS  Google Scholar 

  26. Engel, H W B (1975) Phage typing of Mycobacterzum kansasn, an important aid in studying Its epidemiology Ann Sclavo 17, 578–593

    PubMed  CAS  Google Scholar 

  27. Engel, H. W B, Berwald, L G, Grange, J. M., and Kubm, M (1980) Phage types of Mycobacterzum kansasn Tubercle 61, 11–19.

    Article  PubMed  CAS  Google Scholar 

  28. Baess, I. (1966) A bacteriophage for subdividmg the species M tuberculosu. Am Rev Resp Du 93, 622–623

    CAS  Google Scholar 

  29. Gunnels, J J and Bates, J. H. (1972) Characterization and mycobacteriophage typing of Mycobacterlum xenopz. Am. Rev. Resp Dis 105, 388–392

    PubMed  CAS  Google Scholar 

  30. Rado, T A, Bates, J H, Engel, H W B., Mankiewicz, E, Murohasht, T, Mtzugucht, Y, and Sula, L. (1975) World Health Organization studies on bacteriophage typing of mycobacteria. Subdivision of the spectes Mycobacterzum tuberculosis. Am Rev Resp Dls 111, 459–468.

    CAS  Google Scholar 

  31. Sula, L, Redmond, W. B., Coster, J F., Baess, I., Bates, J. H, Caroh, G, Mankiewlcz, E, Murohashi, T., and Vandra, E (1973) WHO cooperative studies on the phage-typing of mycobacterla 1. Phage lysls of Czechoslovak and Italian strains of Mycobacterzum tuberculoszs Bull World Health Org 48, 57–63.

    PubMed  CAS  Google Scholar 

  32. Saroja, D. and Gopinathan, K. P. (1973) Transduction of lsomazld susceptibility-resistance and streptomycin resistance in mycobacteria Antzmzcro Agents Chemother 4, 643–645.

    CAS  Google Scholar 

  33. Jacobs, W RJr., Barletta, R, Udani, R., Chan, J., Kalkut, G., Sosne, G., Kleser, T., Sarkls, G. J, Hatfull, G. F., and Bloom, B. R. (1993) Rapid assessment of drug susceptibihtles of Mycobacterzum tuberculoszs by means of luclferase reporter phages Sczence 260, 819–822.

    Article  CAS  Google Scholar 

  34. Sarkls, G. J., Jacobs, W R, Jr., and Hatfull, G H (1995) L5 luciferase reporter mycobacterlophages A sensitive tool for the detection and assay of hve mycobacteria. Mol Microbiol 15, 1055–1067.

    Article  Google Scholar 

  35. Pearson, R. E., Jurgensen, S., Sarkis, G J, Hatfull, G. F., and Jacobs, W RJr, (1996) ConstructIon of D29 shuttle phasmids and luclferase reporter phages for detection of mycobactena. Gene 183, 129–136

    Article  PubMed  CAS  Google Scholar 

  36. Snapper, S, Melton, R., Kelser, T, and Jacobs, W. RJr. (1990) Isolation and characterization of efficient plasmid transformation mutants of Mycobacterzum smegmatzs Mol Microbiol 4, 1911–1919.

    Article  PubMed  CAS  Google Scholar 

  37. Tm-nne, T. L. and Brennan, P. J. (1984) Induction of bacteriophage from members of the Mycobacterzum avzum, Mycobacterzum zntracellulare, Mycobacterzum scrofilaceum serocomplex. J Gen Micro 130(8), 2059–2066.

    Google Scholar 

  38. Doke, S. (1960) Studies on mycobacteriophages and lysogemc mycobactena. J KumamotoMed Soc 34, 136–1373

    Google Scholar 

  39. Froman, S., Will, D W, and Bogen, E. (1954) Bacteriophage active against vuulent Mycobacterzum tuberculoszs. I Isolation and activity. Am. J. Pub Health 44, 1326–1334

    Article  PubMed  CAS  Google Scholar 

  40. David, H L, ClCment, F, and Meyer, L (1978) Adsorption of mycobacteriophage D29 on Mycobacterzum leprae. Ann Microbiol. (Inst. Pasteur) 129 A, 563–566.

    Google Scholar 

  41. Redmond, W. B. and Cater, J. C (1960) A bacteriophage specific for Mycobacterzum tuberculoszs, varieties hommus and bovis Am Rev Resp Dzs 82, 781–786

    CAS  Google Scholar 

  42. National Research Council (US) Committee on Hazardous Blologlcal Substances in the Laboratory (1989) Bzosafety zn the Laboratory Prudent Practzces for the Handling of Infectzous Materzals National Academy of Sciences, National Academy Press, Washington, DC

    Google Scholar 

  43. Jacobs, W. R. Jr., Kalpana, G V., Clrillo, J. D., Pascopella, L, Snapper, S B, Udani, R A, Jones, W., Barletta, R. G., and Bloom, B R. (1991) Genetic systems for mycobacterla Methods Enzymol 204, 537–555.

    Article  PubMed  CAS  Google Scholar 

  44. Welgle, J., Meselson, M., and Palgen, K. (1959) Density alterations associated with transducing ability in phage lambda. J Mol Bzol 1, 379–386

    Article  Google Scholar 

  45. Bertani, L.E. and Bertam, G. (1970) Preparation and characterization of temperate, non-inducible bacteriophage P2 (host: Escherzchza coli) J Gen Vzrol 6, 201–212.

    Article  CAS  Google Scholar 

  46. Yamamoto, K. R, Alberts, B M, Benzmger, R, Lawhorne, L., and Treiber, G (1970) Rapid bacteriophage sedimentatton in the presence of polyethylene glycol and its applicatton to large-scale virus purtfication Virology 40, 734–744

    Article  PubMed  CAS  Google Scholar 

  47. Baess, I (1971) Report on pseudolysogenic mycobacterta and a review of the temperature concerning pseudolysogeny Acta pathol et microbiol scandinav B79, 428–434

    Google Scholar 

  48. Grange, J M (1995) Pseudolysogeny in Mycobacterzum dzernhoferz ATCC19341 J Gen Mxrobiol 89, 387–391

    Google Scholar 

  49. Laemmh, U K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680–685

    Article  Google Scholar 

  50. Sambrook, J., Fritsch, E F, and Maniatis, T (1989) Molecular Cloning A Laboratory Manual, 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  51. Arber, W, Enquist, L, Hohn, B, Murray, N. E., and Murray, K. (1983) Experimental methods for use with lambda, in Lambda 11 (Hendrix, R W, Roberts, J. W., Stahl, F. W., and Wetsberg, R A., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 433–466.

    Google Scholar 

  52. Verma, M (1989) Use of ammomum sulfate prectpttation and guamdme tsothiocyanate lysts to isolate lambda DNA. BzoTechnzques 7, 230–232.

    CAS  Google Scholar 

  53. Grange, J M and Redmond, W B. (1978) Host-phage relationships in the genus Mycobacterzum and then clnucal significance Tubercle 59, 203–255

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA

    Gary J. Sarkis & Graham F. Hatfull

Authors
  1. Gary J. Sarkis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Graham F. Hatfull
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. London School of Hygiene and Tropical Medicine, London, UK

    Tanya Parish  & Neil G. Stoker  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Sarkis, G.J., Hatfull, G.F. (1998). Mycobacteriophages. In: Parish, T., Stoker, N.G. (eds) Mycobacteria Protocols. Methods in Molecular Biology™, vol 101. Humana Press. https://doi.org/10.1385/0-89603-471-2:145

Download citation

  • DOI: https://doi.org/10.1385/0-89603-471-2:145

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-471-6

  • Online ISBN: 978-1-59259-576-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation