Abstract
The liver is composed of hepatocytes, endothelial, Kupffer, fat-storing, and pit cells. This chapter describes a technique for isolating hepatocytes, which represent 60–65% of the cells of the liver and about 80% of the volume of the organ (since they are larger than the other cells) (1–3). Two years after Howard et. al. (4) discovered that collagenase was a very important tool in the separation of liver cells, Berry and Friend (5) introduced the technique of collagenase perfusion of the liver.
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References
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© 1997 Humana Press Inc., Totowa, NJ
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Neufeld, D.S. (1997). Isolation of Rat Liver Hepatocytes. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:145
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DOI: https://doi.org/10.1385/0-89603-441-0:145
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-441-9
Online ISBN: 978-1-59259-561-7
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