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Use of Pichia pastoris as a Model Eukaryotic System

Peroxisome Biogenesis

  • Protocol
Pichia Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 103))

Abstract

Many subcellular processes present in multicellular eukaryotes have been shown to reside in yeast as well. The ease with which yeasts are genetically manipulated and analyzed by biochemical and ultrastructural techniques has greatly contributed to the present knowledge of these processes. In particular, the biogenesis, maintenance, and functions of the different subcellular organelles have been intensively studied in yeast. Saccharomyces cerevisiae has been the yeast of choice when the organelles under study are constitutively present in the cell, such as mitochondria, nucleus, Golgi, and the endoplasmic reticulum. In contrast, peroxisome biogenesis is studied in a variety of yeast species, mainly because the abundance of this organelle is highly inducible by external factors. Several nonconventional yeast species show a much more pronounced peroxisome proliferation than does S. cerevisiae (1–6).

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Author information

Authors and Affiliations

  1. Department of Biology, University of California at San Diego, La Jolla, CA, USA

    Klaas Nico Faber, Ype Elgersma, John A. Heyman, Antonius Koller, Georg H. Lüers, William M. Nuttley, Stanley R. Terlecky, Thibaut J. Wenzel & Suresh Subramani

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  1. Klaas Nico Faber
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  2. Ype Elgersma
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  3. John A. Heyman
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  4. Antonius Koller
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  5. Georg H. Lüers
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  6. William M. Nuttley
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  7. Stanley R. Terlecky
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  8. Thibaut J. Wenzel
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  9. Suresh Subramani
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Editor information

Editors and Affiliations

  1. Chiron Technologies/Center for Gene Therapy, San Diego, CA, USA

    David R. Higgins

  2. Oregon Graduate Institute of Science and Technology, Portland, OR, USA

    James M. Cregg

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© 1998 Humana Press Inc., Totowa, NJ

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Faber, K.N. et al. (1998). Use of Pichia pastoris as a Model Eukaryotic System. In: Higgins, D.R., Cregg, J.M. (eds) Pichia Protocols. Methods in Molecular Biology, vol 103. Humana, Totowa, NJ. https://doi.org/10.1385/0-89603-421-6:121

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  • DOI: https://doi.org/10.1385/0-89603-421-6:121

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-421-1

  • Online ISBN: 978-1-59259-578-5

  • eBook Packages: Springer Protocols

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