Abstract
The underlying principle of DNA sequencing by either the Sanger (1) or Maxam and Gilbert method (2), is the ability to fractionate and resolve long, single-stranded DNA molecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on 1-m long gels (3).
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Bankier, A.T. (1996). Electrophoresis of Sequence Reaction Samples. In: Harwood, A.J. (eds) Basic DNA and RNA Protocols. Methods in Molecular Biology™, vol 58. Humana Press. https://doi.org/10.1385/0-89603-402-X:393
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DOI: https://doi.org/10.1385/0-89603-402-X:393
Publisher Name: Humana Press
Print ISBN: 978-0-89603-402-0
Online ISBN: 978-1-59259-251-7
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