Abstract
Successful ribozyme-mediated gene therapy for HIV infection has to take into account several factors:
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1.
The therapeutic gene must interfere with or shut down the expression of essential viral genes.
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2.
This requires a vector system for gene delivery and expression in HIV-sensitive cells.
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3.
To bypass the high genetic variability of the HIV-1 replication process, the target sequence should be chosen in highly conserved sequences of HIV-1. These fortunately are mostly present in the essential regulatory genes (tat, rev) or sequences (PBS, U5), which are required to produce infectious viral particles (1).
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© 1997 Humana Press Inc.
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Cagnon, L., Rossi, J. (1997). Retroviral Delivery and Anti-HIV Testing of Hammerhead Ribozymes. In: Turner, P.C. (eds) Ribozyme Protocols. Methods in Molecular Biology™, vol 74. Humana Press. https://doi.org/10.1385/0-89603-389-9:451
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DOI: https://doi.org/10.1385/0-89603-389-9:451
Publisher Name: Humana Press
Print ISBN: 978-0-89603-389-4
Online ISBN: 978-1-59259-560-0
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