Abstract
A major objective of this laboratory has been to improve the growth performance of swine with immunological manipulation. During the progress of the program, we demonstrated that a mouse monoclonal antibody (MAb) was able to enhance the somatogenic effect of porcine growth hormone (pGH) when administered in an experimental rat model (1,2). However, the concern of this type of approach was that the repeated injections of swine with mouse MAb would induce antimouse Ig responses, thus diminishing the effect of the future applications of the same mouse MAb. A similar problematic situation was clearly evidenced in cancer patients following immunotherapy with mouse MAb (3). Therefore, we attempted to develop MAb of swine origin as an alternative to overcome the potential difficulty in crossing the interspecies barrier.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wang, B. S., Lumanglas, A. L., and Szewczyk, E. (1992) Enhancement of hormonal activity with a monoclonal antibody specific to porcine growth hormone. Anim. Biotechnol. 3, 299–308.
Wang, B. S., Lumanglas, A. L., Szewczyk, E., McWilliams, W., Loullis, C., and Hart, I. C. (1992) A proposed mechanism of action of a growth hormone specific monoclonal antibody in the enhancement of hormonal activity. Mol. Immunol. 29, 313–317.
Shawler, D. L., Bartholomew, R. M., Smith, L. M., and Dillman, R. O. (1985) Human immune response to multiple injections of murine monoclonal IgG. J. Immunol. 135, 1530–1535.
Raybould, T. J. G., Wilson, P. J., McDougall, L. J., and Watts, T. C. (1985) A porcine-murine hybridoma that secretes porcine monoclonal antibody of defined specificity. Am. J. Vet. Res. 46, 1768–1769.
Buchegger, R., Fourier, K., Schreyer, M., Carrel, S., and Mach, J.-P. (1987) Swine monoclonal antibodies of high affinity and specificity to carcino-embryonic antigen. J. Natl. Cancer Inst. 79, 337–342.
Greenlee, A. R., Magnuson, N. S., Smith, C., Butt, B. M., McKiernan, A. J., and Davis, W. C. (1990) Characterization of heteromyeloma fusion partners which promote the outgrowth of porcine hybridomas. Vet. Immunol. Immunopathol. 26, 267–283.
Lumanglas A. L., Sadeghi, H., and Wang, B. S. (1994) Generation of heterohybridomas capable of releasing swine monoclonal antibody specific to porcine growth hormone. Hybridoma 13, 237–240.
Julius, M. H., Simpson, E., and Herzenberg, L. A. (1973) A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur. J. Immunol. 3, 645–649.
Lumanglas, A. and Wang, B. S. (1993) Effect of murine ascites on the ability of hybridoma cells to produce antibody and proliferate in vitro. Hybridoma 12, 127–133.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Humana Press Inc.
About this protocol
Cite this protocol
Lumanglas, A.L., Wang, B.S. (1995). Production of Monoclonal Antibodies in Swine. In: Davis, W.C. (eds) Monoclonal Antibody Protocols. Methods in Molecular Biology™, vol 45. Humana Press. https://doi.org/10.1385/0-89603-308-2:49
Download citation
DOI: https://doi.org/10.1385/0-89603-308-2:49
Publisher Name: Humana Press
Print ISBN: 978-0-89603-308-5
Online ISBN: 978-1-59259-532-7
eBook Packages: Springer Protocols