Abstract
Many of the anticancer chemotherapeutic agents in current clinical use are known to interact strongly with DNA (e.g., Adriamycin, cis-platinum, melphalan, mitomycin C, and mitoxantrone). Although the exact mechanism of action remains unresolved in most cases, the apparent role of DNA has prompted, over several decades, a wide range of studies of these drug-DNA interactions. The major objective of such studies has been to determine the structure of the drug-DNA complex, with the objective being to use this information in the design of new derivatives.
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© 1995 Humana Press Inc.
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Phillips, D.R., Crothers, D.M. (1995). An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNA. In: Tymms, M.J. (eds) In Vitro Transcription and Translation Protocols. Methods in Molecular Biology, vol 37. Humana Press. https://doi.org/10.1385/0-89603-288-4:89
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DOI: https://doi.org/10.1385/0-89603-288-4:89
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