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Principle of Digital Imaging Microscopy

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In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 33))

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Abstract

A challenging problem of in situ hybridization is to visualize then localize genes or specifie sequences within the interphase nuclei or on chromosomes, as we now have at our disposai a large panel of probes. In addition, methods for probe labeling are continuously being improved to allow increased efficiency of in situ hybridization. A considerable advance was recently achieved in chromosome and chromatin mapping by taking advantage of chromatin decondensation (1, 2) and multicolor fluorescence labeling (3–6). Sequences separated by less than 10 kb could be resolved in that way (1, 2).

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Author information

Authors and Affiliations

  1. Unité INSERM 350, Molecular Biophysics,Biology Department, Institut Curie, Paris, France

    Mäité Coppey-Moisan

  2. Laboratory of Radiopathology, Medical Department, Institut Curie, Paris, France

    Jozo Delie & Henri Magdelenat

  3. Department of Biology, Institut Curie, Paris, France

    Jacques Coppey

Authors
  1. Mäité Coppey-Moisan
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  2. Jozo Delie
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  3. Henri Magdelenat
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  4. Jacques Coppey
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Editor information

Editors and Affiliations

  1. Murdoch Institute, Melbourne, Australia

    K. H. Andy Choo

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© 1994 Humana Press Inc.

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Coppey-Moisan, M., Delie, J., Magdelenat, H., Coppey, J. (1994). Principle of Digital Imaging Microscopy. In: Choo, K.H.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 33. Humana Press. https://doi.org/10.1385/0-89603-280-9:359

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  • DOI: https://doi.org/10.1385/0-89603-280-9:359

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-280-4

  • Online ISBN: 978-1-59259-520-4

  • eBook Packages: Springer Protocols

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