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Open Access

Peer-reviewed

Research Article

Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes

Abstract

Streptococcus agalactiae (group B streptococcus or GBS) is a commensal bacterium that can frequently behave as a pathogen, particularly in the neonatal period and in the elderly. The gut is a primary site of GBS colonization and a potential port of entry during neonatal infections caused by hypervirulent clonal complex 17 (CC17) strains. Here we studied the interactions between the prototypical CC17 BM110 strain and polarized enterocytes using the Caco-2 cell line. GBS could adhere to and invade these cells through their apical or basolateral surfaces. Basolateral invasion was considerably more efficient than apical invasion and predominated under conditions resulting in weakening of cell-to-cell junctions. Bacterial internalization occurred by a mechanism involving caveolae- and lipid raft-dependent endocytosis and actin re-organization, but not clathrin-dependent endocytosis. In the first steps of Caco-2 invasion, GBS colocalized with the early endocytic marker EEA-1, to later reside in acidic vacuoles. Taken together, these data suggest that CC17 GBS selectively adheres to the lateral surface of enterocytes from which it enters through caveolar lipid rafts using a classical, actin-dependent endocytic pathway. These data may be useful to develop alternative preventive strategies aimed at blocking GBS invasion of the intestinal barrier.

Citation: De Gaetano GV, Lentini G, Galbo R, Coppolino F, Famà A, Teti G, et al. (2021) Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes. PLoS ONE 16(6): e0253242. https://doi.org/10.1371/journal.pone.0253242

Editor: Roman G. Gerlach, Universitatsklinikum Erlangen, GERMANY

Received: March 12, 2021; Accepted: June 1, 2021; Published: June 15, 2021

Copyright: © 2021 De Gaetano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: Work presented here was supported in part by the PRIN (Programma di Ricerca Scientifica di Rilevante Interesse Nazionale) 2017M8R7N9_002 grant from the Ministero dell’Università and Ricerca Scientifica (MIUR) of Italy. Charybdis Vaccines S.r.l. and Scylla Biotech S.r.l. did not provide funding for this study in any form and had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: Giuseppe Teti and Concetta Beninati act as Scientific Advisors for, respectively, Charybdis Vaccines S.r.l. and Scylla Biotech S.r.l., without receiving any salary or compensation for these activities. These commercial affiliations do not alter the authors’ adherence to PLOS ONE policies on sharing data and materials.

Introduction

Streptococcus agalactiae or group B streptococcus (GBS) is a Gram-positive bacterium that can cause invasive disease, including sepsis and meningitis [1]. Neonatal GBS disease occurring during the first week of life is defined as early-onset disease (EOD) and is often initiated by aspiration of secretions from the genital tract of colonized mothers during parturition [2]. Disease occurring from day 8 to 89 after birth is defined as late onset disease (LOD) [2, 3]. EOD can manifest itself as sepsis, pneumonia and, less commonly, meningitis. In contrast, meningitis is frequent in LOD and can be followed by permanent neurological disorders [4]. GBS infection is increasingly detected in nonpregnant adults and in the elderly and is often associated with serious pathological conditions such as diabetes mellitus, cirrhosis, cancer and endocarditis [5, 6]. GBS frequently colonizes the gut and the genital tract and its ability to adhere to mucosal epithelial cells is an essential factor in these activities [2, 3]. Moreover, invasion of epithelial barriers is an obligatory event in pathogenesis [7, 8].

It has been proposed that the gut is a port of entry of GBS in LOD, which is frequently caused by strains belonging to the CC17 "hypervirulent" clone complex [9, 10]. Indeed, these strains often colonize the infant gut in the first three months of life and can be isolated also from newborns with GBS negative mothers [9]. CC17 strains express a unique set of adhesins and have been described as being particularly capable of invading the endothelial cells of the blood-brain barrier [10]. Their ability to interact with the gut epithelium has received comparatively less attention. In the intact gut mucosa, only the apical membranes of epithelial cells are exposed to the external environment, but, when damaged by microbial toxins or inflammation, lateral surfaces also became available for interactions with bacteria. Interestingly, basolateral cell membranes are more susceptible than apical surfaces to invasion by some bacteria, such as Listeria monocytogenes [11], Shigella flexneri [12] and Campylobacter jejuni but not by other enteropathogens, such as Salmonella spp. [13]. In the present study, we aimed to gain insights into the mechanism of invasion of enterocytes by hypervirulent GBS belonging to the LOD-associated CC17 clonal complex. To this end, we used the Caco-2 cell line that reproduces many features of the natural structure and physiology of the colonic epithelium [14, 15]. Our results indicate that GBS can efficiently invade polarized cells from their lateral surfaces by an actin- and caveolae-dependent mechanism.

Materials and methods

Bacterial strains and reagents

The GBS strain BM110 (serotype III, CC17) and Salmonella enterica serovar Typhimurium (strain M20) were used in the present study. They were grown at 37°C in Todd-Hewitt broth and Luria Bertani broth, respectively (both from Difco Laboratories). Rabbit polyclonal anti-GBS serum was obtained from animals immunized intravenously with formol-inactivated GBS. Rabbit polyclonal anti-EEA-1 antibodies (ab2900) and goat polyclonal anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibodies (ab15007) were obtained from Abcam, Cambridge, UK. Lysotracker Red 500nM (L7526, Invitrogen) was used to label acidified endosomal compartments.

Caco-2 culture

The human intestinal Caco-2 cell line (ATCC HTB-37; colorectal adenocarcinoma) was seeded in 24-well plates at a density of 1 x 104 cells per well in RPMI-1640 medium (R8758, Sigma-Aldrich) supplemented with 10% (vol/vol) fetal bovine serum (1203C, Sigma-Aldrich) and penicillin-streptomycin (100 IU of penicillin and 0.1 mg/ml of streptomycin, P433, Sigma-Aldrich) and cultured at 37°C in a humidified 5% CO2 incubator. The culture medium was changed every three days and cells were used at 5 and 21 days after seeding. Confluence and cellular differentiation were monitored by optical microscopy and immunofluorescence. It was determined in preliminary experiments that Caco-2 cells show similar growth rates, general morphology and intracellular distribution of actin filaments when grown in RPMI-1640 medium supplemented with 10% fetal calf serum or in the medium recommended by ATCC, which contains 20% serum. Previous studies have shown the formation of fully differentiated monolayers with functional intercellular junctions in Caco-2 cells grown under the culture conditions used in the present study [16].

Adhesion and invasion assays

Before each experiment, cultured Caco-2 cells were washed three times and infected with bacteria. Briefly, bacteria were grown to the mid-log phase, collected by centrifugation, washed and resuspended to the concentration of 1x107 CFU/ml in pre-warmed serum-free RPMI. Then bacteria were added to 5-day-old sub-confluent islands or 21-day-old post-confluent monolayers at a MOI of 40. After 1h of incubation at 37°C in the presence of 5% CO2, extracellular bacteria were washed away by rinsing cultured cells three times with Dulbecco’s phosphate buffered saline without calcium and magnesium (DPBS; Euroclone S.p.A., Milan, Italy). The numbers of bacteria attached to the cells were measured by plating cell lysates on blood agar plates, as previously described [17]. To measure bacterial internalization, the infected cells were rinsed three times with DPBS and incubated for an additional 1 h in RPMI containing bactericidal amounts of penicillin and streptomycin (200 U/ml and 200 μg/ml, respectively) as previously described [18]. In some experiments we performed bacterial counts also at other times after infection, as indicated. Adherence and invasion values were expressed as percentage values using the formula: recovered CFU/initial inoculum CFU x 100.

Disruption of intercellular junctions

Monolayers were washed and incubated with DPBS without calcium and magnesium for 1 h to disrupt intercellular junctions. In some experiments, cells were exposed to ethylene glicol-bis-(β-aminoethylether)-N, N, N’, N’-tetraacetic acid (EGTA) (E3889 Sigma-Aldrich) at a 10μM concentration in DPBS for 15 minutes prior to incubation with GBS to induce weakening of cell-to-cell junctions. Treatment with EGTA at this concentration (10μM) did not result in cellular detachment from the plate wells. Weakening of intercellular junctions after treatment with EGTA or Ca++-free medium was confirmed microscopically, as evidenced by gap formation in the monolayers.

Fluorescence microscopy

Structured-illumination fluorescence microscopy analysis was performed using a Zeiss Observer.Z1 Apotome apparatus and images were acquired with the AxionVision software. Caco-2 cells were cultured on 18 mm2 coverslips at a density of 1 x 104 cells per coverslips. Cell growth and confluence were monitored daily. To visualize bacterial adhesion, coverslips were washed at 1 h post-infection and fixed with 3.7% formaldehyde for 15 min at room temperature. After a 1 h blocking step with BSA (2% in DPBS) and several washes, coverslips were sequentially incubated for 1 h with rabbit polyclonal anti-GBS serum and with Alexa Fluor 488-conjugated secondary antibody both diluted 1: 2,000 in DPBS supplemented with BSA 1%. To visualize bacterial invasion, non-adhering bacteria were washed away and cells were further incubated with antibiotics to remove residual extracellular bacteria. Then coverslips were fixed and, after blocking with BSA, permeabilized with 0.1% Triton X-100 for 10 minutes. Phalloidin-iFluor 555 (ab176756, Abcam) and 4′-6-diamidino-2-phenylindole (DAPI) (D8417, Sigma-Aldrich) were used according to the manufacturer’s instructions to label, respectively, actin and DNA (both eukaryotic and bacterial DNA). At least 10 pictures were examined in each experiment.

Endocytosis inhibitors

To study the mechanisms of GBS internalization, Caco-2 cells were treated with the following chemical inhibitors: chlorpromazine (CPZ; C8138-54, Sigma-Aldrich) and monodasylcadaverine (MDC; D4008, Sigma-Aldrich) for inhibition of clathrin-mediated endocytosis; methyl-β-cyclodextrin (MβDC; C4555, Sigma-Aldrich) and genistein (Gen; G6649, Sigma-Aldrich) for inhibition of cholesterol and tyrosine kinase, respectively, in lipid rafts; cytochalasin B (CytB; C6762, Sigma-Aldrich) and nocodazole (Ndz; M1404, Sigma-Aldrich), for inhibiting cellular actin and microtubules, respectively. Cells were pre-incubated with these compounds for 1 h prior to infection at the indicated concentrations. All infections were performed in presence of inhibitors, while control cells were incubated with vehicle only. Epithelial cell viability was evaluated by trypan blue staining after incubation with the inhibitors and was always > 90%.

Statistical analysis

All experiments were repeated at least three times and the data were expressed as means ± standard deviations (SD). Data were analyzed for statistical significance by the Mann-Whitney or the one-way ANOVA tests. A p-value of 0.05 was used as the threshold for significance.

Ethics statement

No in vivo studies were conducted. To obtain rabbit polyclonal anti-GBS serum, rabbits were immunized at the animal facilities of University of Messina, according to the European Union guidelines for the handling of laboratory animals. The procedure was approved by the local animal experimentation committee (Comitato Etico per la Sperimentazione Animale, permit 18052010).

Results

Adhesion of GBS to Caco-2 cells

Growing Caco-2 cells are found in one of three aggregation types, depending on the age of cultures: 1) groups of few undifferentiated cells early after seeding; 2) islands of partially differentiated and polarized cells at approximately 5 days after the beginning of culture (5-day-old islets; panel S1A, S1C and S1E Fig); 3) confluent monolayers of fully differentiated and polarized cells showing the formation of “domes”, consisting of fluid accumulations beneath the monolayer surface (21-day-old monolayers; panel S1B, S1D and S1E Fig) [19, 20]. In initial experiments, we analyzed bacterial adherence using colony counts to determine the numbers of bacteria associated with 5- and 21-day-old cultures. Strikingly, the number of adhering bacteria was considerably higher in 5- than in 21-day-old cultures (Fig 1A). To study the mechanisms underlying these differences, bacterial adherence was analyzed by structured-illumination confocal immunofluorescent microscopy. In 5-day-old islets, a major portion of adherent bacteria was found along the outer edges of the islands, corresponding to the lateral surfaces of polarized cells (Fig 1B). GBS were also found in the intercellular spaces of large 5-day-old islands, particularly in optical sections corresponding to the parabasal levels of the vertical cell axis (S2 Fig). In contrast, in 21-day-old monolayers, only small numbers of bacteria were visible, and these colocalized to apical, but not subapical, sections (Fig 1C). These results indicate that hypervirulent GBS adheres preferentially to the lateral surfaces of Caco-2 cells at subapical and parabasal sites along the vertical cell axis. GBS adhered poorly to mature Caco-2 monolayers, in which subapical sites were not apparently exposed to the external environment. This adherence pattern is reminiscent of that of some enteroinvasive pathogens [11, 12, 21].

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Fig 1. GBS adherence to Caco-2 cells.

GBS adherence in infected islands and monolayers of Caco-2 cells. (A) GBS adherence as measured by CFU counting in 5-day-old islets and 21-day-old monolayers. Shown are means ± SD of three independent experiments conducted in triplicate. *, p<0.05 by Mann-Whitney test. (B) GBS adherence to the outer edges of 5-day-old small islets. Yellow labels indicate the filter(s) used and the distance, in μm, of the optical section from the slide plane. (C) GBS adherence to a small area of the brush border in 21-day-old monolayers (three-dimensional reconstruction). Nuclei and actin filaments were labeled with DAPI (Blue) and Phalloidin-iFluor 555 (Red), respectively. Bacteria (Green) were labeled using a rabbit anti-GBS serum followed by Alexa Fluor 488-conjugated anti-rabbit IgG. Scale bar = 10μm.

https://doi.org/10.1371/journal.pone.0253242.g001

GBS invasion occurs preferentially from the lateral surface of enterocytes

The preferential adherence of bacteria to lateral surface of Caco-2 cells suggested that GBS might invade intestinal epithelial cells from this location. This seemed to be the case, since internalized GBS were found beneath the basolateral membranes in the cells of 5-day-old islands (Fig 2A). As expected, few internalized bacteria were detected in 21-day-old monolayers and these bacteria were localized under the apical cell surface (Fig 2B and 2C). Next, it was of interest to ascertain whether the presence of cell-to-cell junctions prevent GBS access to invasion-permissive sub-apical cell surfaces in monolayers. To this end, epithelial cell junctions were temporarily disrupted by treating cells with medium lacking Ca2+ [22, 23]. This treatment led to a marked increase in bacterial invasion compared to cells treated with a buffer supplemented with calcium and magnesium using both 5- and 21-day-old cultures (Fig 3A and S3C Fig). Another method to disrupt cell-to-cell junctions involves treatment with EGTA, a Ca2+- selective chelating agent. When cells were treated with EGTA, the ability of GBS to adhere to or invade 5- or 21-day-old Caco-2 cultures was considerably increased (Fig 3B, S3A and S3B Fig). To ensure that the ability of EGTA to increase GBS invasiveness was not related to non-specific effects, we used as control Salmonella enterica serovar Typhimurium strain M20, which is able to penetrate Caco-2 cells from the apical side, disrupting large areas of the brush border [24]. Unlike GBS, EGTA treatment did not induce any significant difference in Salmonella enterica serovar Typhimurium invasion of 5-day-old or 21-day-old Caco2 cells (Fig 3C). To ascertain whether GBS actually interact with basolateral cell surfaces after disruption of intracellular junctions, we analyzed EGTA-treated cultures by immunofluorescence microscopy. A considerable number of bacteria was visualized in the basolateral aspect of polarized cells in 5-day-old islands (Fig 3D) and 21-day-old monolayers (Fig 3E). Collectively, these data indicate that GBS can invade polarized Caco-2 cells with great efficiency through the lateral surfaces of the basal pole after disruption of intercellular junctions.

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Fig 2. GBS invasion of 5- and 21-day-old Caco-2 cells.

Fluorescence microscopy was performed on infected Caco-2 cells after killing extracellular bacteria with antibiotics. (A) Equatorial section in the z-stack of a 5-day-old islet with orthogonal views from x/z and y/z planes. (B) 21-day-old monolayer: few internalized GBS (Green) are visible in the apical section of the z-stack. Yellow labels indicate the filter(s) used and distance, in μm, of the optical section from the slide plane. (C) 3D reconstruction of a 21-day-old monolayer shown in panel B with few internalized GBS (green) under the apical surface. DAPI (Blue) and Phalloidin-iFluor 555 (Red) were used for labeling nuclei and actin filaments, respectively. GBS (Green) were visualized with a rabbit anti-GBS serum followed by Alexa Fluor 488-conjugated secondary antibody. Scale bar = 10μm.

https://doi.org/10.1371/journal.pone.0253242.g002

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Fig 3. Enhanced GBS invasion of Caco-2 cells after disruption of intercellular junctions.

Five-day-old islets and 21-day-old monolayers of Caco-2 cells were infected with GBS and extracellular bacteria were killed with antibiotics. Prior to infection, cells were treated as indicated to disrupt intercellular junctions. (A) Effects of Ca2+ and Mg2+ on GBS invasion of 5-day-old islets, as measured by CFU counts in cell lysates. DPBS, Dulbecco’s PBS without Ca2+ and Mg2+. (B) Effect of EGTA on GBS invasion, as measured by CFU counts in cell lysates. (C) Effect of EGTA treatment of Caco-2 cells on S. enterica var. Typhymurium (strain M20) invasion, as measured by CFU counts in cell lysates. Values shown in A, B and C are means ± SD of three independent experiments conducted in triplicate. *, p<0.05 by Mann-Whitney test. (D) Evidence of numerous invading streptococci (Green) in the parabasal region of a 5-day-old islet treated with EGTA. Orthogonal views from x/z and y/z planes are depicted laterally to show the tridimensional location of bacteria. (E) Bacteria interacting with the basal region of 21-day-old monolayers after EGTA treatment. Yellow labels indicate the filter(s) used and the distance, in μm, of the optical section from the slide plane. On the right is a three-dimensional reconstruction centered in the area indicated by the dashed rectangle. Bacteria (Green) were stained with an anti-GBS rabbit serum, followed by Alexa Fluor 488-conjugated anti-rabbit IgG. Actin (Red) and nuclei (Blue) were stained with Phalloidin-iFluor 555 and DAPI, respectively. Scale bar = 10μm.

https://doi.org/10.1371/journal.pone.0253242.g003

GBS internalization involves caveolar lipid rafts

A wide variety of pathogens invade epithelia via microrganism-directed endocytosis, which can be divided in different classes according to the molecular mechanisms involved [25–27]. We focused here on clathrin-, caveolae- and lipid raft-dependent endocytosis, since the majority of pathogenic bacteria use these internalization mechanisms [28–31]. Clathrin-mediated endocytosis can be inhibited by number of agents, including monodansylcadaverine (MDC) and chlorpromazine (CPZ), which reduce clathrin expression at the cell surface. Pretreatment with MDC or CPZ of 5- day-old Caco-2 cell cultures did not affect invasion at any of the tested doses, excluding a major role of chlatrin-mediated internalization in GBS invasion of Caco-2 cells (Fig 4A). Lipid rafts are cholesterol-rich membrane domains performing different functions, including signal transduction and endocytosis [31]. We assessed the role of lipid rafts in GBS internalization using the oligosaccharide methyl-β-cyclodextrin (MβCD), which decreases membrane cholesterol levels and blocks lipid raft functions. Pretreatment of 5- or 21-day-old Caco-2 cultures with MβDC significantly decreased GBS invasion, suggesting a role of lipid rafts in GBS internalization (Fig 4B and S4 Fig). Since lipid rafts can be divided into caveolae-containing and non-caveolar rafts, we examined the role of caveolin in GBS internalization. To this end, we pretreated Caco-2 cells with genistein, an inhibitor of tyrosine kinases involved in caveolae-dependent internalization. Genistein treatment significantly reduced GBS invasion in 5-day-old cultures, indicating that caveolin-dependent endocytosis is at least partially responsible for GBS internalization (Fig 4B and S4 Fig). Collectively, these data indicated that GBS invade cultured enterocyte-like cells by inducing caveolar lipid raft-dependent endocytosis.

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Fig 4. Lipid raft- and caveolin-dependent GBS entry into intestinal cells.

Inhibitory effects of endocytosis-disrupting agents on GBS invasion of 5-day-old Caco-2 islets. Cells were infected with GBS in presence or absence of the inhibitors and intracellular bacteria were enumerated by CFU counts in cell lysates after killing extracellular bacteria with antibiotics. (A) Effect of chlorpromazine (CPZ) and monodansylcadaverine (MDC) at the non-cytotoxic concentrations of 5μM and 25μM, respectively. (B) Effect of inhibition of cholesterol-rich domains and tyrosine kinase on GBS internalization by methyl-betacyclodextrin (MβDC) or genistein (Gen) at the non-cytotoxic concentrations of 100μM and 200μM, respectively. Shown are means ± SD of three independent experiments conducted in triplicate. **, p<0.01 by one-way Anova and Bonferroni test.

https://doi.org/10.1371/journal.pone.0253242.g004

Bacterial internalization normally requires host cytoskeleton rearrangements involving microfilaments, microtubules or both [32–34]. To examine whether the cytoskeleton is required for GBS invasion, Caco-2 cells were treated with cytochalasin B and nocodazole, which specifically inhibit actin filaments and microtubules, respectively. Treatment with cythocalasin B and, to a lesser extent, with nocodazole significantly decreased invasion of BM110 GBS, compared to control cells (Fig 5A). Moreover, immunofluorescence microscopy indicated that at 30 minutes post-infection GBS strongly interact with actin filaments, as indicated by colocalization between bacteria and phalloidin-stained actin at cell entry sites (Fig 5B).

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Fig 5. Actin-dependent invasion of Caco-2 cells by GBS.

(A) Effect of cytochalasin B (CytB) and nocodazole (Ndz) on GBS invasion of 5-day-old Caco-2 islets at the non-cytotoxic concentrations of 50 and 75μM, respectively. Shown are means ± SD of three independent experiments conducted in triplicate **, p<0.01; ***, p<0,001 by one-way Anova and Bonferroni test. (B) Fluorescence microscopy analysis of 5-day-old islets infected with GBS. Scale bar = 10μm. The bottom panels are magnifications of the areas indicated by the two coloured dashed rectangles (orange and green). Cell-associated streptococci appear to be enveloped by actin filaments (red) below the outer edges of the cells. Scale bar = 1μm. Nuclei and nucleoids were stained with DAPI (Blue), actin with Phalloidin-iFluor 555 (Red).

https://doi.org/10.1371/journal.pone.0253242.g005

GBS trafficking and survival inside intestinal epithelial cells

We next investigated the intracellular fate of invading bacteria using immunofluorescence microscopy. At 30 min post-infection, GBS colocalized with EEA-1, a marker of early endosomes (Fig 6A). At later times (i.e. at 1 and 2 h post-infection) GBS predominantly resided in acidified vacuoles, as shown by co-localization with lysotracker, an acidotropic fluorescent die (Fig 6B). Bacterial viability slowly decreased after internalization, although the majority of bacteria were still viable at two hours post-infection (Fig 6C).

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Fig 6. Colocalization of GBS with endosomal markers.

Five-day-old islets were infected with GBS and stained for endosomal markers. (A) Co-localization of invading streptococci (Blue) with the early endosomal marker EEA-1 (Green) and actin filaments (Red) just below the cell membrane at 30 min after infection. Scale bar = 10μm. EEA-1 was stained using rabbit polyclonal antibodies followed by Alexa Fluor 488-conjugated anti-rabbit IgG. The right panels are magnifications of the area indicated by the dashed rectangle. Scale bar = 1μm. (B) Colocalization of GBS (Blue) with acidic endosomes (Red) at 2 h post-infection. White arrows indicate bacteria. The right panels are magnifications of the area indicated by the dashed rectangle. Scale bar = 1μm. (C) CFU counts in cell lysates at different times after treatment of 5-day-old Caco-2 islets with antibiotics to kill extracellular bacteria. Shown are means ± SD of three independent experiments conducted in triplicate. *, p<0.05; by one-way Anova and Bonferroni test.

https://doi.org/10.1371/journal.pone.0253242.g006

Discussion

GBS CC-17, which is strongly associated with LOD, has increased capacities for late colonization of the infant gut, raising the possibility that the intestinal tract is a port of bacterial entry in the pathogenesis of this disease [9, 10]. In humans, the intestinal epithelium performs not only digestive and absorptive functions, but also acts as an extremely efficient physical barrier against pathogens [35, 36]. This barrier function rests, to a great extent, on the integrity of apical-basal polarity and junctional complexes, including tight junctions, adherence junctions, focal adhesions and hemidesmosomes [36–38]. For these reasons, we chose to use, as a model system for the intestinal epithelium, Caco-2 cells, which undergo over time differentiation changes leading to the formation of cell-to-cell junctions, apical brush borders and enterocyte-like morphology [14, 39]. Moreover, to study GBS invasion, we used the prototypical BM110 strain, which is representative of the CC17 clonal complex and displays a unique set of adhesins and virulence factors, comprising both CC17-specific cell wall proteins and adhesins expressed by multiple clonal complexes [9, 18, 40, 41]. The major finding of the present study is that GBS can adhere to and invade enterocytes from their basolateral surfaces in polarized cells. In contrast, invasion from the apical surface was a rare event. S. Typhimurium displayed an opposite behaviour and invaded Caco-2 cells from their apical but not lateral surfaces, confirming the specificity of our findings. The ability of GBS to preferentially invade the lateral surface of enterocytes is shared by Listeria monocytogenes [11], Shigella flexneri [12] and Campylobacter jejuni [13], but not by other enteropathogens, such as Salmonella spp. or E.coli [24, 42].

In the present study, GBS invaded Caco-2 cells with great efficiency when cell-to-cell junctions were disrupted by Ca2+ depletion, but not using intact monolayers. Our data suggest that the GBS receptors on intestinal cells are selectively distributed in the lateral surface of the basal cell pole and are at least partially hidden in intact monolayers. This raises the question as to how GBS can gain access to their receptors when cell-to-cell junctions are intact in the normal mucosa. In this respect, at least two possibilities can be envisioned. It is likely, in the first place, that GBS temporally affect the integrity of cell-to cell junctions. This possibility is supported by observations that GBS intimately associate with and transiently open tight junctions in monolayers of cervical and Caco-2 cells, as shown by transmission electron microscopy [43]. Accordingly, in the present study, GBS were often found along the lateral surface of partially differentiated cells. In the second place, cell-to-cell contacts loosen up to allow passage of cells and fluids in the intestinal lumen under inflammatory conditions. Although GBS carriage is not commonly regarded as being accompained with innate immune responses, recent data point to the occurrence of inflammatory phenomena during colonization, at least in the vaginal habitat [44–46]. Elevated levels of IL-1α were detected in GBS-positive non-pregnant women [44]. Moreover, increased production of other pro-inflammatory cytokines and neutrophil accumulation were documented in mouse models of vaginal colonization [46]. Future studies should assess whether GBS colonization of the gut is accompanied with an innate immune responses and should establish the impact, if any, of such responses on the barrier function of the intestinal epithelium.

Of course, our data showing invasion from the lateral surfaces of enterocytes do not exclude the existence of other mechanisms for invasion of the intestinal barrier by GBS. It has been shown, for example, that this pathogen can use a paracellular route to transverse Caco-2 monolayers growing on filters [43]. Moreover, CC17 GBS can apparently cross the mouse intestinal barrier using M cells, which are specialized intestinal epithelial cells with a marked ability to sample luminal contents and deliver it to the underlying mucosal tissues [47]. Indeed, transcytosis through M cells has been shown to occur in vivo for Shigella and other enteropathogens [48]. Therefore, it appears that GBS might invade the intestinal barrier through multiple pathways. An important point to be addressed in future studies will concern the nature of the GBS receptor that mediates preferential invasion through the lateral surface of enterocytes. A number of potential bacterial receptors are known to be expressed at specific locations on the cell membrane of these cells. E-cadherin is restricted to the cell-cell border of intestinal epithelial cells [49] and acts as a ligand for listerial internalin A, which can explain the preferential internalization of L. monocytogenes at the lateral enterocyte surface [50]. The α5β1 integrin, which can act as a receptor for CC17 GBS in brain endothelial cells [51], is also found on the lateral surfaces of enterocytes and on the apical surfaces of M cells [52]. Studies are underway to ascertain if CC17 uses the α5β1 integrin and other integrins to invade the lateral surface of enterocytes and cross the intestinal barrier.

In the present study, adherence of GBS to the lateral surfaces of Caco-2 cells was followed by caveolin- and lipid raft-dependent endocytosis, while clathrin-mediated internalization was not apparently involved. Similar to GBS, a number of other pathogens, such as Streptococcus uberis, Chlamydia trachomatis and Pseudomonas aeruginosa, were reported to invade epithelial through caveolae-dependent mechanisms [53–56]. We show here that caveolar lipid raft-dependent endocytosis results in close association of GBS with actin filaments and internalization in EEA1-positive early endosomes, followed by their rapid passage into lysotracker-positive, acidified compartments. This localization resembles the classical endosomal pathway used by many pathogens to reside more or less temporarily in intestinal cells [56, 57]. In conclusion, we demonstrate that GBS can invade polarized enterocytes through an actin- and caveolar lipid raft-dependent mechanism after adhering to the basolateral portion of the cell surface. Future studies should assess the relative importance of this invasion pathway in vivo. These data may be useful to develop alternative strategies to prevent LOD by blocking GBS invasion of the intestinal barrier.

Supporting information

S1 Fig. Morphological features of 5-day- and 21-day-old Caco-2 cells.

(A) Cellular islands of different dimensions at 5 days post-seeding (100x, optical microscopy). (B) “Dome” structures in completely differentiated monolayers at 21 days post-seeding (200x, optical microscopy). (C) Fluorescence microscopy analysis of 5-day-old islands. Left panels show an islet consisting of few cells, while right panels show a larger island. Microvilli are evident in the right lower panel. (D) Fluorescence microscopy analysis of 21-day-old monolayers with a well established dotted brush border. Nuclei and actin filaments were labeled with DAPI and Phalloidin-iFluor 555, respectively. Fluorescence microscopy was performed using a Zeiss Observer.Z1 microscope with an Apotome apparatus and were acquired at the z-stack positions shown in (E). Scale bar = 10μm.

https://doi.org/10.1371/journal.pone.0253242.s001

(TIF)

S2 Fig. GBS cell adherence in a large 5-day-old island.

Fluorescence microscopy analysis of an infected 5-day-old island. The top left panel was obtained by integrating 18 optical sections taken at various distances along the z axis using the Extended Focus (EF) module of the AxioVision software. The other panels represent single optical sections taken at the indicated levels along the z axis. Orthogonal views from x/z and x/y planes are also shown. Yellow labels indicate the distance of the optical section from the slide plane. Scale bar = 10μm. Nuclei and nucleoids were stained with DAPI (Blue), actin with Phalloidin-iFluor 555 (Red) and bacteria with rabbit anti-GBS serum followed by an Alexa Fluor 488-conjugated anti-rabbit IgG (Green).

https://doi.org/10.1371/journal.pone.0253242.s002

(TIF)

S3 Fig. Weakening of intercellular junctions enhances GBS interactions with Caco-2 cells.

Effect of EGTA treatment of 5- (A) or 21-day-old (B) Caco-2 cell cultures on GBS adherence as measured by CFU counts. C) Effect of cell treatment with media with or without Ca2+ and Mg2+ on GBS invasion using 21-day-old monolayers, as measured by CFU counts in cell lysates. DPBS, Dulbecco’s PBS without Ca2+ and Mg2+. Shown are means + SD of three independent experiments conducted in triplicate. *, p<0.05 by Mann-Whitney test.

https://doi.org/10.1371/journal.pone.0253242.s003

(TIF)

S4 Fig. GBS internalization in EGTA-treated cells from 21-day old monolayers involves lipid raft- and caveolin-dependent mechanisms.

Inhibitory effects of endocytosis-disrupting agents on GBS cell invasion in 21-day-old monolayers. Prior to infection, cells were treated with EGTA to disrupt intercellular junctions. Infection was performed in presence or absence of the inhibitors and intracellular bacteria were enumerated by CFU counts in cell lysates after killing extracellular bacteria with antibiotics. Shown are the effects on GBS internalization of inhibition of cholesterol-rich domains and tyrosine kinase by, respectively, methyl-betacyclodextrin (MβDC) or genistein (Gen) at the non-cytotoxic concentrations of 100 μM and 200 μM, respectively. Shown are means ± SD of three independent experiments conducted in triplicate. *, p<0.05; **, p<0.01 by one-way ANOVA and Bonferroni test.

https://doi.org/10.1371/journal.pone.0253242.s004

(TIF)

References

  1. 1. Verani JR, McGee L, Schrag SJ (2010) Prevention of perinatal group B streptococcal disease—revised guidelines from CDC, 2010. MMWR Recomm Rep 59: 1–36.
  2. 2. Melin P (2011) Neonatal group B streptococcal disease: from pathogenesis to preventive strategies. Clinical Microbiology and Infection 17: 1294–1303. pmid:21672083
  3. 3. Hon KL, Chan KH, Ko PL, So KW, Leung AKC (2017) Late Onset Streptococcus agalactiae Meningitis following Early Onset Septicemia: A Preventable Disease? Case Reports in Pediatrics 2017: 8418105. pmid:29098104
  4. 4. Melin P, Efstratiou A (2013) Group B streptococcal epidemiology and vaccine needs in developed countries. Vaccine 31 Suppl 4: D31–42.
  5. 5. Shabayek S, Spellerberg B (2018) Group B Streptococcal Colonization, Molecular Characteristics, and Epidemiology. Front Microbiol 9: 437. pmid:29593684
  6. 6. Farley MM, Strasbaugh LJ (2001) Group B Streptococcal Disease in Nonpregnant Adults. Clinical Infectious Diseases 33: 556–561. pmid:11462195
  7. 7. Buscetta M, Firon A, Pietrocola G, Biondo C, Mancuso G, et al. (2016) PbsP, a cell wall-anchored protein that binds plasminogen to promote hematogenous dissemination of group B Streptococcus. Molecular Microbiology 101: 27–41. pmid:26888569
  8. 8. Vornhagen J, Waldorf KMA, Rajagopal L (2017) Perinatal Group B Streptococcal Infections: Virulence Factors, Immunity, and Prevention Strategies. Trends in Microbiology 25: 919–931. pmid:28633864
  9. 9. Tazi A, Plainvert C, Anselem O, Ballon M, Marcou V, et al. (2019) Risk Factors for Infant Colonization by Hypervirulent CC17 Group B Streptococcus: Toward the Understanding of Late-onset Disease. Clin Infect Dis 69: 1740–1748. pmid:30946447
  10. 10. Tazi A, Disson O, Bellais S, Bouaboud A, Dmytruk N, et al. (2010) The surface protein HvgA mediates group B streptococcus hypervirulence and meningeal tropism in neonates. The Journal of experimental medicine 207: 2313–2322. pmid:20956545
  11. 11. Gaillard JL, Finlay BB (1996) Effect of cell polarization and differentiation on entry of Listeria monocytogenes into the enterocyte-like Caco-2 cell line. Infect Immun 64: 1299–1308. pmid:8606093
  12. 12. Mounier J, Vasselon T, Hellio R, Lesourd M, Sansonetti PJ (1992) Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole. Infect Immun 60: 237–248. pmid:1729185
  13. 13. Bouwman LI, Niewold P, van Putten JPM (2013) Basolateral Invasion and Trafficking of Campylobacter jejuni in Polarized Epithelial Cells. PLOS ONE 8: e54759. pmid:23382959
  14. 14. Pinto M (1983) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol cell 47: 323–330.
  15. 15. Natoli M, Leoni BD, D’Agnano I, D’Onofrio M, Brandi R, et al. (2011) Cell growing density affects the structural and functional properties of Caco-2 differentiated monolayer. Journal of Cellular Physiology 226: 1531–1543. pmid:20945374
  16. 16. Cotter P, Kleiveland C, Lea T, López-Expósito In, Mackie A, et al. The Impact of Food Bioactives on Health: in vitro and ex vivo models. 1st ed. pp. 1 online resource (XVII, 338 pages 357 illustrations, 335 illustrations in color.).
  17. 17. Papasergi S, Garibaldi M, Tuscano G, Signorino G, Ricci S, et al. (2010) Plasminogen- and Fibronectin-binding Protein B Is Involved in the Adherence of Streptococcus pneumoniae to Human Epithelial Cells. Journal of Biological Chemistry 285: 7517–7524. pmid:20048164
  18. 18. Lentini G, Midiri A, Firon A, Galbo R, Mancuso G, et al. (2018) The plasminogen binding protein PbsP is required for brain invasion by hypervirulent CC17 Group B streptococci. Scientific Reports 8: 14322. pmid:30254272
  19. 19. Delie F, Rubas W (1997) A human colonic cell line sharing similarities with enterocytes as a model to examine oral absorption: advantages and limitations of the Caco-2 model. Crit Rev Ther Drug Carrier Syst 14: 221–286. pmid:9282267
  20. 20. Ramond MJ, Martinot-Peignoux M, Erlinger S (1985) Dome formation in the human colon carcinoma cell line Caco-2 in culture. Influence of ouabain and permeable supports. Biology of the Cell 54: 89–92. pmid:3161570
  21. 21. Cerquetti M, Serafino A, Sebastianelli A, Mastrantonio P (2002) Binding of Clostridium difficile to Caco-2 epithelial cell line and to extracellular matrix proteins. FEMS Immunology & Medical Microbiology 32: 211–218.
  22. 22. Fleiszig SM, Evans DJ, Do N, Vallas V, Shin S, et al. (1997) Epithelial cell polarity affects susceptibility to Pseudomonas aeruginosa invasion and cytotoxicity. Infect Immun 65: 2861–2867. pmid:9199460
  23. 23. Pitelka DR, Taggart BN, Hamamoto ST (1983) Effects of extracellular calcium depletion on membrane topography and occluding junctions of mammary epithelial cells in culture. J Cell Biol 96: 613–624. pmid:6403552
  24. 24. Lhocine N, Arena ET, Bomme P, Ubelmann F, Prévost M-C, et al. (2015) Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton. Cell host & microbe 17: 164–177. pmid:25600187
  25. 25. Lin AE, Guttman JA (2010) Hijacking the endocytic machinery by microbial pathogens. Protoplasma 244: 75–90. pmid:20574860
  26. 26. Alonso A, García-del Portillo F (2004) Hijacking of eukaryotic functions by intracellular bacterial pathogens. Int Microbiol 7: 181–191. pmid:15492932
  27. 27. Roy CR, van der Goot FG (2003) Eukaryotic cells and microbial pathogens: a familiar couple take centre stage. Nat Cell Biol 5: 16–19. pmid:12511887
  28. 28. Veiga E, Cossart P (2006) The role of clathrin-dependent endocytosis in bacterial internalization. Trends Cell Biol 16: 499–504. pmid:16962776
  29. 29. Lajoie P, Nabi IR (2010) Lipid rafts, caveolae, and their endocytosis. Int Rev Cell Mol Biol 282: 135–163. pmid:20630468
  30. 30. Pizarro-Cerdá J, Bonazzi M, Cossart P (2010) Clathrin-mediated endocytosis: what works for small, also works for big. Bioessays 32: 496–504. pmid:20486136
  31. 31. Simons K, Toomre D (2000) Lipid rafts and signal transduction. Nat Rev Mol Cell Biol 1: 31–39. pmid:11413487
  32. 32. Morey P, Cano V, Martí-Lliteras P, López-Gómez A, Regueiro V, et al. (2011) Evidence for a non-replicative intracellular stage of nontypable Haemophilus influenzae in epithelial cells. Microbiology (Reading) 157: 234–250. pmid:20929955
  33. 33. Dhakal BK, Mulvey MA (2009) Uropathogenic Escherichia coli invades host cells via an HDAC6-modulated microtubule-dependent pathway. J Biol Chem 284: 446–454. pmid:18996840
  34. 34. Dramsi S, Cossart P (1998) Intracellular pathogens and the actin cytoskeleton. Annu Rev Cell Dev Biol 14: 137–166. pmid:9891781
  35. 35. Backert S, Boehm M, Wessler S, Tegtmeyer N (2013) Transmigration route of Campylobacter jejuni across polarized intestinal epithelial cells: paracellular, transcellular or both? Cell Communication and Signaling 11: 72. pmid:24079544
  36. 36. Snoeck V, Goddeeris B, Cox E (2005) The role of enterocytes in the intestinal barrier function and antigen uptake. Microbes Infect 7: 997–1004. pmid:15925533
  37. 37. Cavallaro U, Christofori G (2004) Cell adhesion and signalling by cadherins and Ig-CAMs in cancer. Nat Rev Cancer 4: 118–132. pmid:14964308
  38. 38. de Pereda JM, Ortega E, Alonso-García N, Gómez-Hernández M, Sonnenberg A (2009) Advances and perspectives of the architecture of hemidesmosomes: lessons from structural biology. Cell adhesion & migration 3: 361–364. pmid:19736524
  39. 39. van Breemen RB, Li Y (2005) Caco-2 cell permeability assays to measure drug absorption. Expert Opin Drug Metab Toxicol 1: 175–185. pmid:16922635
  40. 40. Périchon B, Szili N, du Merle L, Rosinski-Chupin I, Gominet M, et al. (2017) Regulation of PI-2b Pilus Expression in Hypervirulent Streptococcus agalactiae ST-17 BM110. PLOS ONE 12: e0169840. pmid:28107386
  41. 41. De Gaetano GV, Pietrocola G, Romeo L, Galbo R, Lentini G, et al. (2018) The Streptococcus agalactiae cell wall-anchored protein PbsP mediates adhesion to and invasion of epithelial cells by exploiting the host vitronectin/α(v) integrin axis. Mol Microbiol 110: 82–94. pmid:30030946
  42. 42. Robins-Browne RM, Hartland EL (2002) Escherichia coli as a cause of diarrhea. J Gastroenterol Hepatol 17: 467–475. pmid:11982729
  43. 43. Soriani M, Santi I, Taddei A, Rappuoli R, Grandi G, et al. (2006) Group B Streptococcus crosses human epithelial cells by a paracellular route. J Infect Dis 193: 241–250. pmid:16362888
  44. 44. Nikolaitchouk N, Andersch B, Falsen E, Stroembeck L, Mattsby-Baltzer I (2008) The lower genital tract microbiota in relation to cytokine-, SLPI- and endotoxin levels: application of checkerboard DNA-DNA hybridization (CDH). Apmis 116: 263–277. pmid:18397461
  45. 45. Patras KA, Rösler B, Thoman ML, Doran KS (2015) Characterization of host immunity during persistent vaginal colonization by Group B Streptococcus. Mucosal Immunol 8: 1339–1348. pmid:25850655
  46. 46. Carey AJ, Tan CK, Mirza S, Irving-Rodgers H, Webb RI, et al. (2014) Infection and cellular defense dynamics in a novel 17β-estradiol murine model of chronic human group B streptococcus genital tract colonization reveal a role for hemolysin in persistence and neutrophil accumulation. J Immunol 192(4):1718–31. pmid:24453257
  47. 47. Hays C, Touak G, Bouaboud A, Fouet A, Guignot J, et al. (2019) Perinatal hormones favor CC17 group B Streptococcus intestinal translocation through M cells and hypervirulence in neonates. eLife 8: e48772. pmid:31710290
  48. 48. Sansonetti PJ, Phalipon A (1999) M cells as ports of entry for enteroinvasive pathogens: Mechanisms of interaction, consequences for the disease process. Seminars in Immunology 11: 193–203. pmid:10381865
  49. 49. Hermiston ML, Gordon JI (1995) In-Vivo Analysis of Cadherin Function in the Mouse Intestinal Epithelium—Essential Roles in Adhesion, Maintenance of Differentiation, and Regulation of Programmed Cell-Death. Journal of Cell Biology 129: 489–506.
  50. 50. Wells CL, van de Westerlo EMA, Jechorek RP, Haines HM, Erlandsen SL (1998) Cytochalasin-induced actin disruption of polarized enterocytes can augment internalization of bacteria. Infection and Immunity 66: 2410–2419. pmid:9596696
  51. 51. de Cambronne RD, Fouet A, Picart A, Bourrel AS, Anjou C, et al. (2021) CC17 group B Streptococcus exploits integrins for neonatal meningitis development. Journal of Clinical Investigation 131.
  52. 52. Hamzaoui N KS, Caliot E, Pringault E. (2004) Expression and distribution of beta1 integrins in in vitro-induced M cells: implications for Yersinia adhesion to Peyer’s patch epithelium. Cellular Microbiology 6(9):817–828. pmid:15272863
  53. 53. Almeida RA, Dunlap JR, Oliver SP (2010) Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells. Veterinary medicine international 2010: 319192–319192. pmid:20614000
  54. 54. Norkin LC, Wolfrom SA, Stuart ES (2001) Association of caveolin with Chlamydia trachomatis inclusions at early and late stages of infection. Exp Cell Res 266: 229–238. pmid:11399051
  55. 55. Zaas DW, Swan ZD, Brown BJ, Li G, Randell SH, et al. (2009) Counteracting signaling activities in lipid rafts associated with the invasion of lung epithelial cells by Pseudomonas aeruginosa. The Journal of biological chemistry 284: 9955–9964. pmid:19211560
  56. 56. Duncan MJ, Shin J-S, Abraham SN (2002) Microbial entry through caveolae: variations on a theme. Cellular Microbiology 4: 783–791. pmid:12464009
  57. 57. Cossart P, Sansonetti PJ (2004) Bacterial invasion: the paradigms of enteroinvasive pathogens. Science 304: 242–248. pmid:15073367