Abstract
Gas chromatography with electron capture detection has been applied for the determination of T-2 toxin (T-2) in traditional Chinese herbal medicines (TCHM). The method consists of extracting the sample with aqueous methanol followed by cleanup of the resulting extract with an immunoaffinity column. T-2 was determined as its heptafluorobutyl ester. The reaction temperature and time of derivatization were investigated to obtain the optimum conditions. Recoveries from different TCHMs, spiked with T-2 at levels ranging from 50 to 1,000 μg kg−1, were from 82.2 to 98.6%, with relative standard deviations of less than 7.5%. The limit of detection was 2.5 μg kg−1. Out of 32 commercially available TCHM samples analyzed, none were found to contain any detectable amount of T-2.
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Acknowledgments
This work was supported by Fundamental Scientific Research Project of China (YZ-1-20), Important New Drug Research Project of the Ministry of Science and Technology of China (2009ZX09502-025) and Scientific Research Project of Traditional Chinese Medicine Vocation in 2008 (200807042).
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Yue, YT., Zhang, XF., Ou-Yang, Z. et al. Determination of T-2 Toxin in Traditional Chinese Herbal Medicines by GC-ECD. Chroma 70, 1495–1499 (2009). https://doi.org/10.1365/s10337-009-1330-6
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DOI: https://doi.org/10.1365/s10337-009-1330-6