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Quantitative Determination of Eight Major Constituents in the Traditional Chinese Medicinal Yi-Qi-Fu-Mai Preparation by LC

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Abstract

A high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of eight marker components, ginsenoside Rf, Rb1, Rb2, Rb3, Rd, Rg3, 20(S)-ginsenoside F2 and schizandrin, present in traditional Chinese medicinal Yi-Qi-Fu-Mai preparations. Samples were prepared using a solid phase extraction procedure. Chromatographic separation was carried out on a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) by stepwise gradient elution with water (0.05% phosphoric acid, v/v) and acetonitrile as the mobile phase. Good linear relationships between were observed between peak areas and concentrations with r 2 values above 0.9990 for all the analytes. Average recoveries for the eight markers ranged from 97.7 to 100.4%. This method was found to be rapid, sensitive, accurate, and could be readily applied to the quality assessment of Yi-Qi-Fu-Mai preparations.

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Authors and Affiliations

  1. Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Cai Lun Road 1200, 201203, Shanghai, People’s Republic of China

    Dan-Dan Zhou & Zheng-Tao Wang

  2. National Institute for the Control of Pharmaceutical and Biomedical Products, Tiantan Xili 2, 100050, Beijing, People’s Republic of China

    Dan-Dan Zhou, Shou-Jun Jiang, Gang-Li Wang & Rui-Chao Lin

  3. Shenyang Pharmaceutical University, Wen Hua Road 103, 110016, Shenyang, Liaoning, People’s Republic of China

    Ling Tong

  4. Tianjin Tasly Pride Pharmaceutical Company Limited, Pujihe East Road 2, 300402, Tianjin, People’s Republic of China

    Yue-Wu Yang & Zheng-Liang Ye

Authors
  1. Dan-Dan Zhou
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  2. Shou-Jun Jiang
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  3. Ling Tong
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  4. Yue-Wu Yang
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  5. Gang-Li Wang
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  6. Zheng-Liang Ye
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  7. Zheng-Tao Wang
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  8. Rui-Chao Lin
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Correspondence to Rui-Chao Lin.

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Zhou, DD., Jiang, SJ., Tong, L. et al. Quantitative Determination of Eight Major Constituents in the Traditional Chinese Medicinal Yi-Qi-Fu-Mai Preparation by LC. Chroma 70, 969–974 (2009). https://doi.org/10.1365/s10337-009-1252-3

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  • DOI: https://doi.org/10.1365/s10337-009-1252-3

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