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LC Method for the Determination of Meropenem in Cerebrospinal Fluid: Application to Therapeutic Drug Monitoring

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Abstract

A simple, rapid and accurate liquid chromatography method using ultrafiltration to pretreat cerebrospinal fluid (CSF) samples was developed to determine meropenem concentrations in human CSF in clinical settings. Meropenem in CSF samples was stabilized by mixing with 1 mol L−1 3-morpholinopropanesulfonic acid buffer (pH 7.0) (1:1). The mixture was transferred to a Nanosep 10 K centrifugal filter device; after centrifugation, the filtrate was subjected to reversed-phase LC and the eluate was monitored at 300 nm. The retention time for meropenem was 5.8 min. The calibration curve of meropenem in human CSF was linear over 0.05–50 μg mL−1. The intra-day and inter-day precision was 0.27–5.66 % and accuracy was 99.0–109.5 %. The limit of detection was 0.01 μg mL−1. This method was successfully applied to neurosurgical patients, showing that it is applicable for therapeutic drug monitoring in patients receiving meropenem.

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Authors and Affiliations

  1. Department of Clinical Pharmacotherapy, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan

    Tomoko Matsuda, Kazuro Ikawa, Kayo Ikeda & Norifumi Morikawa

  2. Department of Neurosurgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan

    Ryu Tsumura, Masaaki Shibukawa, Koji Iida & Kaoru Kurisu

Authors
  1. Tomoko Matsuda
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  2. Kazuro Ikawa
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  3. Kayo Ikeda
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  4. Norifumi Morikawa
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  5. Ryu Tsumura
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  6. Masaaki Shibukawa
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  7. Koji Iida
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  8. Kaoru Kurisu
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Correspondence to Kazuro Ikawa.

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Matsuda, T., Ikawa, K., Ikeda, K. et al. LC Method for the Determination of Meropenem in Cerebrospinal Fluid: Application to Therapeutic Drug Monitoring. Chroma 69, 1031–1034 (2009). https://doi.org/10.1365/s10337-009-1013-3

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  • DOI: https://doi.org/10.1365/s10337-009-1013-3

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