Skip to main content
SpringerLink
Log in

LC Analysis of Oxytetracycline and Chlortetracycline: Application for In Vitro Bio-Equivalence Study of Veterinary Medicines

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A liquid chromatographic method has been applied for the analysis of two antibiotics widely used in the veterinary field, oxytetracycline dihydrate and chlortetracycline hydrochloride in premixes for medicated feeding stuffs for veterinary use. In particular, the validated method was employed to study the releasing profile of each drug from two formulations, a commercially available and a new formulation, having different excipient composition. The dissolution profiles obtained from the chromatographic analysis allowed to verify the in vitro bio-equivalence of the commercial and the new formulations for oxytetracycline and chlortetracycline.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Costa P, Lobo J (2001) Eur J Pharm Sci 13:123–133. doi:10.1016/S0928-0987(01)00095-1

    Article  CAS  Google Scholar 

  2. Liang Y, Bouner Denton M, Bates RB (1998) J Chromatogr A 827:45–55. doi:10.1016/S0021-9673(98)00755-9

    Article  CAS  Google Scholar 

  3. Oka H, Ito Y, Matsumoto H (2000) J Chromatogr A 882:109–133. doi:10.1016/S0021-9673(99)01316-3

    Article  CAS  Google Scholar 

  4. European Pharmacopoeia (2008) 6th edn

  5. The United States Pharmacopoeia (USP26) (2003)

  6. Hoogmartens J, Khan NH, Vanderhaeghe H, Van der Leeden AL, Oosterbaan M, Veld-Tulp GL et al (1989) J Pharm Biomed Anal 7:601–610. doi:10.1016/0731-7085(89)80226-2

    Article  CAS  Google Scholar 

  7. Ding X, Mou S (2000) J Chromatogr A 897:205–214. doi:10.1016/S0021-9673(00)00779-2

    Article  CAS  Google Scholar 

  8. Cherlet M, Schelkens M, Cronbles S, De Baker P (2003) Anal Chim Acta 492:199–213. doi:10.1016/S0003-2670(03)00341-6

    Article  CAS  Google Scholar 

  9. Skùlason S, Ingòlfosson E, Kristmundsdòttir T (2003) J Pharm Biomed Anal 33:667–672. doi:10.1016/S0731-7085(03)00316-9

    Article  Google Scholar 

  10. Oka H, Ikai Y, Ito Y, Hayakawa J, Harada K, Suzuki M et al (1997) J Chromatogr B Analyt Technol Biomed Life Sci 693:337–344. doi:10.1016/S0378-4347(97)00076-5

    Article  CAS  Google Scholar 

  11. Yekkala R, Diana J, Adams E, Roets E, Hoogmartens J (2003) Chromatographia 58:313–316

    CAS  Google Scholar 

  12. Lykkeberg AK, Halling-Sorensen B, Cornett C, Tjornelund J, Hansen SH (2004) J Pharm Biomed Anal 34:325–332. doi:10.1016/S0731-7085(03)00500-4

    Article  CAS  Google Scholar 

  13. Fiori J, Grassigli G, Filippi P, Gotti R, Cavrini V (2005) J Pharm Biomed Anal 37:979–985. doi:10.1016/j.jpba.2004.06.017

    Article  CAS  Google Scholar 

  14. Monser L, Darghouth F (2000) J Pharm Biomed Anal 23:353–362. doi:10.1016/S0731-7085(00)00329-0

    Article  CAS  Google Scholar 

  15. Nelis H, De Leenheer A (1980) J Chromatogr A 195:35–42. doi:10.1016/S0021-9673(00)81541-1

    Article  CAS  Google Scholar 

  16. Böcker R (1980) J Chromatogr A 187:439–441. doi:10.1016/S0021-9673(00)80479-3

    Article  Google Scholar 

  17. Hon J, Murray L (1982) J Liq Chromatogr 5:1973–1990. doi:10.1080/01483918208062867

    Article  CAS  Google Scholar 

  18. European Pharmacopoeia Dissolution test for solid dosage forms, 01/2005:20903, V edn

  19. European Pharmacopoeia Soya-bean oil, hydrogenated, 01/2005:1265 and Soya-bean oil refined, 01/2005:1473, V edn

Download references

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy

    Francesca Mancini, Cristina Cavallari, Lorenzo Rodriguez, Anna Maria Di Pietra & Vincenza Andrisano

  2. Industria Italiana Integratori TREI S.p.A, Viale Corassori 62, 41100, Modena, Italy

    Paola Filippi

Authors
  1. Francesca Mancini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cristina Cavallari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paola Filippi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lorenzo Rodriguez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anna Maria Di Pietra
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vincenza Andrisano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vincenza Andrisano.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mancini, F., Cavallari, C., Filippi, P. et al. LC Analysis of Oxytetracycline and Chlortetracycline: Application for In Vitro Bio-Equivalence Study of Veterinary Medicines. Chroma 69, 215–220 (2009). https://doi.org/10.1365/s10337-008-0874-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0874-1

Keywords

Search

Navigation